Construct: ORF TRCN0000475687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000211.1_s317c1
- Derived from:
- ccsbBroadEn_08619
- DNA Barcode:
- CCGTTATCTACAGATAATCAAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGB3 (56102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56102 | PCDHGB3 | protocadherin gamma subfami... | NM_018924.5 | 99.9% | 100% | 2721G>A |
| 2 | human | 56102 | PCDHGB3 | protocadherin gamma subfami... | NM_032097.3 | 86.5% | 86.2% | (many diffs) |
| 3 | human | 5098 | PCDHGC3 | protocadherin gamma subfami... | NM_032403.3 | 13.7% | 13.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2856
- ORF length:
- 2787
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaaatagc tccggatgga ggggcccagc agggcagagg cgaatgctat 121 ttctcttcct gctctctttg ttagaccagg ctctctccga accgatccgc tacgctattc 181 ccgaggagct ggacaggggc tcgctggtag ggaacctcgc caaggacctg gggtttggcg 241 tgggggattt acctactagg aacctgcggg ttattgcaga gaagaaattc tttaccgtga 301 gccccgaaaa tgggaactta cttgtgagcg accgtataga ccgagaggag atttgtggca 361 agaagtcgac gtgtgttctg gaatttgaaa tggttgctga aaagccttta aacttttttc 421 atgtaactgt gctgatccag gatattaacg acaacccacc gacctttagc caaaatatca 481 ctgagctgga aatcagcgaa ctggctctca ctggagccac atttgccctg gaatctgcgc 541 aagatcctga tgtaggtgtc aattcgctgc agcagtacta cctcagccct gatccgcact 601 tctctttgat tcagaaggag aacctggatg gcagtaggta cccagagcta gtactgaaag 661 cacccctgga cagggaagag cagccacatc accacctggt cctcacagct gtggatgggg 721 gcgagccctc cagaagctgt accacccaga tcagggtaat tgtcgcagat gcaaatgata 781 accccccagt atttactcag gacatgtaca gggtcaatgt tgcagagaac ctgcccgctg 841 gctcctccgt attaaaagtg atggccattg acatggatga gggcatcaat gccgaaatca 901 tctatgcctt catcaatatt ggcaaggaag tgagacaact gttcaagctg gacagtaaaa 961 cgggggaact caccactatt ggagaactgg actttgaaga gagagatagc tacacaattg 1021 gggtggaagc aaaggatggt ggacatcaca ctgcatattg taaagtacag atagatattt 1081 cagatgaaaa tgacaatgcc ccggagataa ccctggcttc tgaatcccaa catatacaag 1141 aagatgctga gctggggact gccgttgccc tgatcaaaac acatgatcta gattctggat 1201 ttaatggaga aatcctatgc caactaaaag gaaacttccc ctttaaaatc gttcaagata 1261 ccaaaaacac atacaggttg gtgacagatg gagccctgga ccgggagcag atcccagaat 1321 acaatgtgac gatcacagct accgacaaag gcaatccacc gctctcctcc agcaagacca 1381 tcactctgca catccttgat gtcaacgaca acgttcccgt tttccaccag gcctcctaca 1441 ccgtgcatgt agctgagaac aatccgcctg gagcctccat tgcgcatgtc agagcctcgg 1501 atcccgactt gggacctaat ggccttgtct cctactacat cgtggccagt gacctggagc 1561 cgcgggagct gtcgtcctac gtgtccgtga gcgcgcggag cggggtggtg ttcgcgcagc 1621 gagccttcga ccacgagcag ctgcgtgcct tcgagctcac tctgcaggcc cgcgaccagg 1681 gctcgcctac gctcagcgcc aacgtgagcc tgcgcgtgtt ggtggacgac cgcaacgaca 1741 atgcaccgct ggtgctgtac ccagctctgg ggcccgaagg ctctgcgctc ttcgatatgg 1801 tgccgcgctc tgcagagcct ggctacctgg tgaccaaggt ggtggcggtg gacgcagact 1861 cgggatacaa cgcctggctg tcctaccaca ttgtgcaggc cagcgagccc gggctgttca 1921 gcctgggcct gcgcacgggt gaggtgcgca cggcgcgtac cttgggcgac agggaggccg 1981 cccgccagcg cctgctggtc actgtgcgtg atggaggaca gcagcctctt tcagccaccg 2041 tcatgctgca cctaatcttc gcagatagct tgcaagagat acaacctgac cttagcgacc 2101 gccccactcc ctctgaccct caggcggagc tacagtttca cctagtagtg gcgttggcct 2161 tgatctcagt gctcttcctc ctcgcggtga ttctggcaat ctccctgcgc ctgcgatgct 2221 cctccagacc cgccactgag ggctactttc agcctggtgt ctgcttcaag actgtacctg 2281 gagttctccc cacctacagc gaaaggactt tgccttattc ctacaatccg tgtgctgcct 2341 cacattcctc aaacaccgag tttaaatttc tcaatataaa ggctgaaaat gctgcaccac 2401 aagatcttct atgtgatgaa gcctcttggt ttgaaagtaa tgacaatcca gaaatgcctt 2461 ctaattcagg caatttgcaa aagcaagccc cgcccaacac ggactggcgt ttctcTCAGG 2521 CCCAGAGACC CGGCACCAGC GGCTCCCAAA ATGGCGATGA CACCGGCACC TGGCCCAACA 2581 ACCAGTTTGA CACAGAGATG CTGCAAGCCA TGATCTTGGC GTCCGCCAGT GAAGCTGCTG 2641 ATGGGAGCTC CACCCTGGGA GGGGGTGCCG GCACCATGGG ATTGAGCGCC CGCTACGGAC 2701 CCCAGTTCAC CCTGCAGCAC GTGCCCGACT ACCGCCAGAA TGTCTACATC CCAGGCAGCA 2761 ATGCCACACT GACCAACGCA GCTGGCAAAC GGGATGGCAA GGCCCCAGCA GGTGGCAATG 2821 GCAACAAGAA GAAGTCGGGC AAGAAGGAGA AGAAGTTGCC AACTTTCTTG TACAAAGTGG 2881 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2941 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3001 ACCGTTATCT ACAGATAATC AAGTCACGCG TTAAGTCgac aatcaacctc tggattacaa 3061 aatttgtgaa agatt