Construct: ORF TRCN0000475693
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008722.1_s317c1
- Derived from:
- ccsbBroadEn_14072
- DNA Barcode:
- CAAAATATCGTACATTACTGCAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RAB3GAP1 (22930)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475693
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | XM_011510825.3 | 95.1% | 75.9% | (many diffs) |
| 2 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | NM_012233.3 | 93.6% | 75.8% | (many diffs) |
| 3 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | NM_001172435.2 | 92.9% | 75.3% | (many diffs) |
| 4 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | XM_011510823.3 | 90% | 72.6% | (many diffs) |
| 5 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | XR_001738674.2 | 76.4% | (many diffs) | |
| 6 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | NM_178690.4 | 81.4% | 70.7% | (many diffs) |
| 7 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | XM_006529405.2 | 80.9% | 70.2% | (many diffs) |
| 8 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | XR_001784075.1 | 60.3% | (many diffs) | |
| 9 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | XR_001784076.1 | 60% | (many diffs) | |
| 10 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | XM_006529407.3 | 53.3% | 40.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2319
- ORF length:
- 2250
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttctcactc ataagtggta tatttacttc tggcacatgg gaagagaaat 121 cagatgaaat ttcctttgct gacttcaagt tctcagtcac tcatcattat cttgtacaag 181 agtccactga taaagaagga aaggatgagt tattagagga tgttgttcca caatctatgc 241 aagatttgct gggtatgaat aatgactttc ctccaagagc acattgcctg gtaagatggt 301 atgggctacg tgagttcgtg gtgattgccc ctgctgcaca cagtgacgct gttctcagcg 361 aatctaagtg caaccttctt ctgagttctg tttctattgc cttgggaaac actggctgtc 421 aggtgccact ctttgtgcaa attcaccaca aatggcgaag aatgtatgta ggagaatgtc 481 aaggtcctgg tgtacgaact gatttcgaaa tggttcatct tagaaaagtg ccaaatcagt 541 acactcactt atcaggtctg ctggatatct tcaaatcaaa gattggatgt cctttaactc 601 cattgcctcc agttagtatt gctattcgat ttacctatgt acttcaagat tggcagcagt 661 atttttggcc tcagcaacct ccagacatag atgcccttgt aggaggagaa gttggaggct 721 tggagtttgg caagttacca tttggtgcct gcgaagatcc tattagtgaa ctccatttag 781 ctactacatg gcctcatctg accgaaggga tcattgtgga taatgatgtt tattctgatt 841 tggatcctat tcaagctcca cattggtctg ttagagttcg aaaagctgag aatcctcagt 901 gtttgctagg tgattttgtc actgaatttt ttaaaatttg ccgtcgaaag gagtcaactg 961 atgagattct tggacgatct gcatttgagg aagaaggcaa agaaactgct gatataactc 1021 atgctttgtc aaaattgaca gagccggcat cagttccaat tcataaatta tcagtttcaa 1081 atatggtaca cactgcaaag aagaaaatcc gaaaacacag aggtgtagag gagtcaccgc 1141 taaataatga tgttcttaat actattctcc tgttcttatt ccctgatgct gtttctgaga 1201 aaccattaga tggaactact tcaacagata ataataatcc tccatcagag agtgaagact 1261 ataatctcta caatcagttc aagtctgcac catctgacag tttaacacac aaactggctt 1321 tgtgtctctg tatgatcaat ttttaccatg gagggttgaa aggagtggca cacctctggc 1381 aggaatttgt tcttgaaatg cgtttccgat gggaaaacaa ctttctgatt ccaggattag 1441 caagtggacc cccagatctg aggtgttgtt tactgcatca gaaactacag atgttaaatt 1501 gttgtattga aagaaagaag gcacgtgatg aggggaaaaa gacaagtgct tcagatgtca 1561 ctaatatata tccaggggat gctggaaaag caggagacca gttggtgcca gataatctaa 1621 aagaaacaga taaggaaaag ggagaggtag gaaaatcttg ggattcctgg agtgacagcg 1681 aagaagaatt ttttgaatgc ctaagtgata ctgaagaact taaaggaaat ggacaagaga 1741 gtggcaagaa aggaggacct aaggagatgg caaatttaag gccggaagga cggctgtatc 1801 agcatgggaa acttacactg ctgcataatg gagaacctct ctacattcca gtaacccagg 1861 aaccagcacc tatgacagaa gatctgctag aagagcagtc tgaagtttta gctaaattag 1921 gtacatcggc agagggggct caccttcgag cacgcatgca gagtgcctgt ctgctctcag 1981 atatggagtc ctttaaggca gctaatccag gttgctccct ggaagatttt gtgaggtggt 2041 attcaccccg ggattatatt gaagaggagg tgattgatga aaagggcaat gtggtgctga 2101 aaggagaact gagtgcccgg atgaagattc caagcaatat gtgggtagaa gcctgggaaa 2161 cagctaagcc aattcctgct agaaggcaaa ggagactctt tgatgataca cgggaagcag 2221 aaaaggtgct gcactatctg gcaatccaga aacctgcaga ccttgctcgg cacctgttac 2281 cttgtgtgat tcatgcagct gtactcaagg taaaggaata agaaagtctc gaaaacattt 2341 cttcagttaa gaagatcata aagcagataa tatcccattc cagtaaagtt ttgcacttcc 2401 ccaatccaga agacaagaaa ttggaagaaa tcattcacca gattactaat gtggaagctc 2461 tcattgccag agctcggtca ctaaaagcca agtttggaac tgagaaatgt gaacaggagg 2521 aggaaaagga agatcttgaa aggtttgtga gttgcctgct ggagcagccT GAAGTGTTAG 2581 TCACCGGTGC AGGAAGAGGA CATGCTGGCA GGATCATTCA CAAGCTGTTT GTGAATGCCC 2641 AGAGGGCTGC AGCTATGACT CCACCAGAGG AGGAATTGAA GAGAATGGGC TCCCCAGAGG 2701 AAAGAAGGCA GAACTCCGTG TCAGACTTCC CACCCCCTGC TGGCCGGGAA TTCATTTTGC 2761 GCACCACTGT GCCGCGCCCT GCTCCCTACT CCAAAGCTCT GCCTCAGCGG ATGTACAGTG 2821 TTCTCACCAA AGAGGACTTT AGACTTGCAG TTAAAATAAT CGATGGGGAT GTGTTGCCAA 2881 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2941 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 3001 TATCTTGTGG AAAGGACGAC AAAATATCGT ACATTACTGC AATACGCGTT AAGTCgacaa 3061 tcaacctctg gattacaaaa tttgtgaaag att