Construct: ORF TRCN0000475714
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000084.1_s317c1
- Derived from:
- ccsbBroadEn_13531
- DNA Barcode:
- GATATTATAATTATCCGTCACCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP4A22 (284541)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475714
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | XR_001737005.1 | 88.3% | (many diffs) | |
| 2 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | NM_001010969.4 | 87.2% | 86.3% | (many diffs) |
| 3 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NM_000778.4 | 85.3% | 83.2% | (many diffs) |
| 4 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NM_001319155.2 | 79.5% | 77.8% | (many diffs) |
| 5 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | NM_001308102.1 | 77.3% | 68.3% | (many diffs) |
| 6 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | XM_005270539.1 | 75.7% | 65.7% | (many diffs) |
| 7 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | XM_005270767.3 | 67.8% | 60% | (many diffs) |
| 8 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NM_001363587.2 | 66.4% | 57.7% | (many diffs) |
| 9 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | XM_017000465.1 | 66.3% | 65.5% | (many diffs) |
| 10 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | XM_005270770.3 | 56.9% | 46.6% | (many diffs) |
| 11 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134992.2 | 48% | (many diffs) | |
| 12 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | XR_001737122.1 | 47.5% | (many diffs) | |
| 13 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134994.2 | 46.7% | (many diffs) | |
| 14 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134989.2 | 46.6% | (many diffs) | |
| 15 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134990.2 | 46.4% | (many diffs) | |
| 16 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134991.2 | 45.8% | (many diffs) | |
| 17 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134988.2 | 39.1% | (many diffs) | |
| 18 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134993.2 | 36.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1434
- ORF length:
- 1365
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgtctct gtcctgagcc ccagcagacg cctgggtggt gtctccggga 121 tcctccaagt gacctccctg ctcattctgc ttctgctgct gatcaaggca gctcagctct 181 acctgcatag gcagtggctg ctcaaagccc tccagcagtt cccgtgccct ccctcccact 241 ggctcttcgg gcacatccag gagttccaac acgaccagga gctacaacgg attcaggaac 301 gggtgaagac attcccaagt gcctgtcctt attggatatg gggaggcaaa gttcgtgtcc 361 agctctatga ccctgactat atgaaggtga ttctggggag atcagacccg aaatcccatg 421 gatcctacaa attcctggct ccatggattg ggtacagctt gctcctgttg aatgggcaga 481 catggttcca gcatcgacgg atgctgaccc cagccttcca ctatgacatc ctgaagccat 541 acgtggggct catggcagac tctgtacgag tgatgctgga caaatgggaa gagctccttg 601 gccaggattc ccctctggag ttctttcagc acgtctcctt gatgaccctg gacaccatca 661 tgaagagtgc cttcagccat cagggcagca tccaggtgga caggaattct cagtcctaca 721 tccaggccat tagtgacctg aacagcctgg ttttttgccg tatgaggaat gcctttcatg 781 agaatgacac catctacagc ctgacctctg ctggccgctg gacacaccgc gcctgccagc 841 tggcccatca gcacacagac caagtgatcc aactgaggaa ggctcaacta cagacggagg 901 gggagctgga gaagatcaag aggaagaggc acttggattt tctggacatc ctcctcttgg 961 ccaaaatgga gaatgggagc atcttgtcag acaaggacct ccgtgctgag gtggacacgt 1021 tcatgtttga gggccacgac accacagcca gtgggatctc ctggatcctc tatgctctgg 1081 ccacacaccc caagcatcag gagaggtgcc gggaggagat ccatggcctc cTGGGTGATG 1141 GAGCCTCCAT CACCTGGAAC CACCTGGACC AGATGCCCTA CACCACCATG TGCATTAAGG 1201 AGGCACTGAG GCTCTACCCA CCGGTGCCAG GCATTGGAAG AGAGCTCAGC ACTCCCGTCA 1261 CCTTCCCTGA TGGGCGCTCC TTGCCCAAAG GTATCATGGT CCTCCTCTCC ATTTATGGCC 1321 TTCACCACAA CCCAAAAGTG TGGCCCAACC CAGAGGTGTT TGACCCTTCC CGTTTTGCAC 1381 CGGGTTCTGC TCAACACAGC CACGCTTTCC TGCCCTTCTC AGGAGGATCA AGGTTGCCAA 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAG ATATTATAAT TATCCGTCAC CATACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att