Construct: ORF TRCN0000475722
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012677.1_s317c1
- Derived from:
- ccsbBroadEn_04972
- DNA Barcode:
- TTGTGTATCCTCCCTATATCATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM120B (144404)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475722
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144404 | TMEM120B | transmembrane protein 120B | NM_001080825.2 | 100% | 100% | |
| 2 | human | 144404 | TMEM120B | transmembrane protein 120B | XM_024448851.1 | 72.8% | 52.8% | 551_552ins128;741_742ins148 |
| 3 | human | 144404 | TMEM120B | transmembrane protein 120B | XM_024448852.1 | 59.6% | 54.4% | (many diffs) |
| 4 | mouse | 330189 | Tmem120b | transmembrane protein 120B | NM_001039723.2 | 88.9% | 94.3% | (many diffs) |
| 5 | mouse | 330189 | Tmem120b | transmembrane protein 120B | XM_011248230.2 | 73.7% | 77.8% | (many diffs) |
| 6 | mouse | 330189 | Tmem120b | transmembrane protein 120B | XM_006530390.3 | 68.8% | 74.3% | (many diffs) |
| 7 | mouse | 330189 | Tmem120b | transmembrane protein 120B | XR_001784711.1 | 50.8% | (many diffs) | |
| 8 | mouse | 330189 | Tmem120b | transmembrane protein 120B | XR_387570.3 | 50.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1086
- ORF length:
- 1017
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccgggcag ctggagcgtt gcgagcgcga atggcacgag ctggagggag 121 aatttcaaga actgcaggag acgcacagga tctacaagca gaagctggag gagctggctg 181 cgctgcagac gctgtgtagc agttccatca gtaagcagaa gaagcacctc aaggacttga 241 agcttacact ccagaggtgc aaacgccatg ccagtcggga ggaggcggag ctcgttcagc 301 agatggcagc gaacatcaag gagcggcagg acgtcttctt cgacatggag gcctacctgc 361 ccaagaagaa cgggctctac ttgaacctgg tcctcggcaa tgtgaacgtg accctcctca 421 gcaaccaggc caagttcgcc tacaaggacg aatatgagaa gttcaagctc tacctgacca 481 tcatcctgct cctgggtgcc gtggcatgtc gatttgtcct tcactacagg gtgactgacg 541 aagtcttcaa cttcctgctg gtgtggtatt actgcaccct gaccattcgg gagagcattc 601 tcatcagcaa cggctcaaga attaaaggct ggtgggtgtc tcaccactac gtctccacat 661 tcctgtccgg agtgatgctg acctggccta atggacccat ttatcagaag tttcgcaacc 721 agttcttagc attttccatt ttTCAGAGCT GCGTCCAGTT CCTGCAATAT TATTACCAGA 781 GGGGCTGCCT CTACCGGCTG CGGGCCCTGG GGGAGAGGAA CCACCTGGAT CTCACAGTGG 841 AAGGGTTCCA GTCCTGGATG TGGCGGGGCC TCACCTTTCT CCTGCCCTTC CTCTTCTGTG 901 GCCATTTCTG GCAGCTCTAC AATGCCGTCA CGCTGTTTGA GCTCTCCAGC CACGAGGAAT 961 GCAGAGAATG GCAGGTGTTC GTACTGGCGT TCACCTTCCT CATCCTCTTC CTCGGCAACT 1021 TCCTGACCAC GCTCAAAGTC GTGCATGCCA AGCTCCAGAA GAACAGAGGC AAGACAAAGC 1081 AGCCGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTGTGTATC CTCCCTATAT CATTTACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt