Construct: ORF TRCN0000475731
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015200.1_s317c1
- Derived from:
- ccsbBroadEn_09754
- DNA Barcode:
- GTCGCCTAGCGGGCTAAGTGACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KANK4 (163782)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475731
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163782 | KANK4 | KN motif and ankyrin repeat... | NM_181712.5 | 99.6% | 99.6% | (many diffs) |
| 2 | human | 163782 | KANK4 | KN motif and ankyrin repeat... | XM_011540843.3 | 99.6% | 99.6% | (many diffs) |
| 3 | human | 163782 | KANK4 | KN motif and ankyrin repeat... | XM_011540845.3 | 97.2% | 97.2% | (many diffs) |
| 4 | human | 163782 | KANK4 | KN motif and ankyrin repeat... | XM_017000483.2 | 92.9% | 93% | (many diffs) |
| 5 | human | 163782 | KANK4 | KN motif and ankyrin repeat... | NM_001320269.2 | 36.5% | 36% | (many diffs) |
| 6 | human | 163782 | KANK4 | KN motif and ankyrin repeat... | XM_017000484.2 | 36.5% | 36% | (many diffs) |
| 7 | human | 163782 | KANK4 | KN motif and ankyrin repeat... | XM_017000485.2 | 35.1% | 34.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3051
- ORF length:
- 2985
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaagacagat gccaaagacc agtcctctca gggggatgaa gagaaagacc 121 ctccgaagag ccacccttat tctgtggaga ccccatatgg ctttcattta gacctggact 181 tcctcaagta tgtggatgac atcgagaagg gaaacactat caaaagaatt cctatccaca 241 gaagggccaa gcaggccaaa tttagcactc tgccccgaaa cttcagcctt cctgacagtg 301 gggctcgccc ccctgcagcc ccgcccctcc aaaactggtc tcccgtggtg ccaagggagg 361 catcacttgg gacacaggag caaaaccagt cgccgccgct tggtaatgcc ccccaggcct 421 caacaagcag gagtgaggtg agctaccaca ggaaggctct gttggcagag gccaccagac 481 agttggaagc tgctgagcca gaggatgccg agctcacttt tgggagtgga cggccccagc 541 tcttgagagc atccagcatg cctgccacgc tgctgcacag cagggcttct gaggagccag 601 gcctgagcct ggggccccct gcccctcctg ccctccctcc ccttcagggt gaaggcagtg 661 tctgtgatgg cacctttgaa cctgcagaag gattggcagg tttccacagc tccagcccac 721 gagcatcaac tcggattcca gagctggtcc aggagggagc tgagcctcca gagggcgtgg 781 tgaaggttcc aaatcacctc cctctcccag gccctccttt ctcattccag aatgtgcttg 841 tagttctaga ggacaaggaa gatgaacaca atgccagaga agcagaggtg ttgttcaccc 901 ctggctcccc tacgccaagc ccgccacctc tgccatcacc catccctgag aatgagctcc 961 tcctggaaga aatcgagctc aacatcagcg agattccacc cccgccacct gtagaggtgg 1021 acatgagaag cattggcatc agggtaactg aggaaagcct gggccttgcc agggtggatc 1081 caggcagcat ctccagcctg aaacagcagg tctcggccct ggagggagag ttgtctggaa 1141 gaaccgagga actggcacag gtcagaactg ctctccagca gcaggaagag gaaatcaaag 1201 ctagggagca aagaattcga gagctggagt tcactgtagc ccaactggaa ggacagtttc 1261 accaagagaa cgccaaagac actcagggcc agacggacgt gatggtgaac actgaccctg 1321 tccatggact cttgaccagg gagtcgtgtg ataagggcat tgaagtcaac cttctaggca 1381 gcatggagtc tgaaagctgg gggcaccgag gagaggagaa tggcctccta tgggggccag 1441 atggtcataa acaagggaat cagagcccag cagaacgtgt gcttctgccc cagctgtcac 1501 tgccacaggg acccgagcag gtccttacct cctctgtaca tagcttcctc tccactgaac 1561 tcaggattga agaagcaggc actgaacagg aaggaggccc tcagggagga accaggggag 1621 caggaggctt tctgtggggc agtgacagaa agactccccc agcagggagg gaggagacca 1681 gttccaatct cccagggaag gagcacccgg gaaggccacc aagctcgcca acggatgcca 1741 ctattgggca gtatgtgaag aagatccagg agctcctgca ggagcagtgg aactgcctgg 1801 agcatgggta cccggagctg gccagcgcca tcaagcagcc agcctccaag ctcagcagca 1861 tccagagcca gctgctgagc tccctcaacc tgctgctgtc ggcctactcg gcccaggctc 1921 acccacccaa ggagccgccg gcctcctcct cctccccgcc agtggagatc tccccatcga 1981 ccagccttaa atccataatg aaaaagaaag actatggctt ccgtgcagga ggtaatggga 2041 ccaaaaagaa ccttcagttt gttggggtta acggtgggta tgagaccacc tcaagtgagg 2101 agaccagcgg tgaggacagc accccagagg acttgtctga cagcgaggca gagaagaagt 2161 gtgacggccc agatcacaag catgtcaaag atgcccatct cacctgcgag gctgggcagg 2221 gcatccctga gggcacctgc catgctgccc aggaaagtgg gcctggggaa gaagtccccc 2281 actccaaggc cgagagatat aaaccctcag aagaatttct taatgcatgc cgggcactga 2341 gccagcatct gccagaaact gggaccacca cagaccagct cttgaggcaa agcttgaaca 2401 ccatcagtca agagtggttc cgcgtctcca gccggaagtc gtctagccct gccgtggtgg 2461 cctcctacct ccacgaggtc cagcctcact ccccacactt cctgaagctg cttgtcaact 2521 tggccgatcg caacgggaac acggcccttc actacagcgt gtcccactcc aacttctcca 2581 tcgcgaagct gctgctggag acaggcgtct gcaatgtgga ccatcagaac aaagctggct 2641 acactgccgt aatgatcact cccttggctt ccgcagagac caatgaagac atggctgttg 2701 tctggaagct cttaagagaa ggaaatgtga acattcaagc tacTCAGGGA GGCCAGACTG 2761 CGCTGATGCT GGGAGTCAGC CACGACAGGG AGGACATGGT TCAAGCGCTG CTTAGCTGCC 2821 AGGCAGATGT CAATCTGCAG GACCACGATG GATCCTCGGC CCTCATGCTG GCCTGTCACC 2881 ATGGCAACGT GGACCTGGTG CGGCTGCTCC TGGCACACCC AGCCTGCGAC AGCAGCCTGA 2941 CTGACAAGGC TGGCCGCACA GCTTTGTCCA TCGCTCTGAA GTCACCCACC CATATGGAAA 3001 TTGCTGGGCT TCTGAGAGCC CACGCGGAGC AGGGCAGGTC CCTGGGGCTG TACCCAACTT 3061 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 3121 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 3181 CTTGTGGAAA GGACGAGTCG CCTAGCGGGC TAAGTGACGC ACGCGTTAAG TCgacaatca 3241 acctctggat tacaaaattt gtgaaagatt