Construct: ORF TRCN0000475757
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013056.1_s317c1
- Derived from:
- ccsbBroadEn_14055
- DNA Barcode:
- ATTATTGTCCTGCGTTTGCATAGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARPP19 (10776)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475757
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10776 | ARPP19 | cAMP regulated phosphoprote... | NM_001306195.1 | 99.7% | 99.1% | 336_337insC |
| 2 | human | 10776 | ARPP19 | cAMP regulated phosphoprote... | NM_006628.6 | 99.7% | 99.1% | 336_337insC |
| 3 | human | 10776 | ARPP19 | cAMP regulated phosphoprote... | NM_001330309.1 | 85.4% | 84.9% | 0_1ins48;288_289insC |
| 4 | human | 10776 | ARPP19 | cAMP regulated phosphoprote... | XM_017021870.2 | 85.4% | 84.9% | 0_1ins48;288_289insC |
| 5 | human | 10776 | ARPP19 | cAMP regulated phosphoprote... | NM_001306191.1 | 85.2% | 84.8% | 44_100del;393_394insC |
| 6 | human | 10776 | ARPP19 | cAMP regulated phosphoprote... | NM_001306196.2 | 85.2% | 84.8% | 44_100del;393_394insC |
| 7 | mouse | 59046 | Arpp19 | cAMP-regulated phosphoprote... | NM_021548.4 | 90.8% | 98.2% | (many diffs) |
| 8 | mouse | 59046 | Arpp19 | cAMP-regulated phosphoprote... | XM_006511327.1 | 90.8% | 98.2% | (many diffs) |
| 9 | mouse | 59046 | Arpp19 | cAMP-regulated phosphoprote... | NM_001142655.1 | 70.1% | 76% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 405
- ORF length:
- 339
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgcggaagtc cccgaggcag cctccgcgga ggagcagaag gaaatggaag 121 ataaagtgac tagtccagag aaagcagaag aagcaaaatt aaaagcaaga tatcctcatc 181 TGGGACAAAA GCCTGGAGGT TCAGATTTCT TAAGGAAACG GTTGCAGAAA GGGCAAAAAT 241 ATTTTGATTC TGGGGATTAC AACATGGCTA AAGCAAAAAT GAAGAACAAG CAACTTCCTA 301 CTGCAGCTCC GGATAAGACG GAGGTCACTG GTGACCACAT TCCCACTCCG CAAGACCTTC 361 CTCAACGGAA GCCGTCCCTT GTTGCTAGCA AGCTGGCTGG CCTGCCCAAC TTTCTTGTAC 421 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 481 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 541 AAGGACGAAT TATTGTCCTG CGTTTGCATA GGACGCGTTA AGTCgacaat caacctctgg 601 attacaaaat ttgtgaaaga tt