Construct: ORF TRCN0000475791
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011614.1_s317c1
- Derived from:
- ccsbBroadEn_05773
- DNA Barcode:
- AAAAGGGTTAAATCTTGTTTCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADCY9 (115)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475791
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115 | ADCY9 | adenylate cyclase 9 | NM_001116.4 | 99.9% | 100% | (many diffs) |
| 2 | human | 115 | ADCY9 | adenylate cyclase 9 | XM_005255079.3 | 98.5% | 98.6% | (many diffs) |
| 3 | human | 115 | ADCY9 | adenylate cyclase 9 | XM_011522353.2 | 70.9% | 69.9% | (many diffs) |
| 4 | mouse | 11515 | Adcy9 | adenylate cyclase 9 | NM_009624.3 | 86.6% | 92.1% | (many diffs) |
| 5 | mouse | 11515 | Adcy9 | adenylate cyclase 9 | XM_011245805.1 | 85.5% | 90.8% | (many diffs) |
| 6 | mouse | 11515 | Adcy9 | adenylate cyclase 9 | XM_011245806.1 | 85.5% | 90.8% | (many diffs) |
| 7 | mouse | 11515 | Adcy9 | adenylate cyclase 9 | NM_001291910.1 | 71.3% | 76% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4128
- ORF length:
- 4059
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttccccg ccccaccagc agctgctgca tcaccacagc accgaggtga 121 gctgcgactc cagcggggac agcaacagcg tgcgcgtcaa gatcaacccc aagcagctgt 181 cctccaacag ccaccccaag cactgcaaat acagcatctc ctctagctgc agcagctctg 241 gggactccgg gggcgtcccc cggcgagtgg gcggcggagg ccggctgcgc aggcagaaga 301 agctgcccca gctgttcgag agggcctcca gccgctggtg ggaccccaag ttcgactcgg 361 tgaacctgga ggaggcctgc ctggagcgct gcttcccgca gacccagcgc cggttccggt 421 atgcgctctt ctacatcggc ttcgcctgcc ttctgtggag catctatttt gcggtccaca 481 tgagatccag actgatcgtc atggtcgccc ccgcgctgtg cttcctcctg gtgtgtgtgg 541 gcttctttct gtttaccttc accaagctgt acgcccggca ttacgcgtgg acctcgctgg 601 ctctcaccct gctggtgttc gccctgaccc tggctgcgca gttccaggtc ttgacgcctg 661 tctcaggacg cggcgacagc tccaacctta cggccacagc ccggcccaca gatacttgct 721 tatctcaagt ggggagcttc tccatgtgca tcgaagtgct ctttttgctc tataccgtca 781 tgcacttacc tttgtacctg agtttgtgtc tgggggtggc ctactctgtc cttttcgaga 841 cctttggcta ccatttccgg gatgaagcct gcttcccctc gcccggagcc ggggccctgc 901 actgggagct gctgagcagg gggctgctcc acggctgcat ccacgccatc ggggtccacc 961 tgttcgtcat gtcccaggtg aggtccagga gcaccttcct caaggtgggg caatccatta 1021 tgcacgggaa ggacctggaa gtggaaaaag ccctcaaaga gaggatgatt cattccgtga 1081 tgccaagaat catagccgat gacttaatga agcagggaga tgaggagagt gagaattctg 1141 tcaagagaca tgccacctcg agccccaaga acaggaagaa aaagtcttcc atccaaaaag 1201 ctcctatagc cttccgccct tttaagatgc agcagatcga agaagtcagt attttatttg 1261 cagatatcgt gggcttcacc aagatgagtg ccaacaagtc tgcccacgcc ctggtgggtc 1321 tcctgaacga tctgttcggt cgcttcgacc gcctgtgtga ggagaccaag tgtgagaaaa 1381 tcagcaccct gggagactgt tactactgcg tggcgggctg tcccgagccc cgggccgacc 1441 atgcctactg ctgcatcgag atgggcctgg gcatgatcaa ggccatcgag cagttctgcc 1501 aggagaagaa ggagatggtg aacatgagag tcggggtgca cacgggcacc gtcctttgcg 1561 gcatcctggg catgaggagg tttaaatttg acgtgtggtc caacgatgtg aacctggcca 1621 atctcatgga gcagctggga gtggccggca aagttcacat ttctgaggcc accgcaaaat 1681 acttagatga ccggtacgaa atggaagatg ggaaagttat tgaacggctg ggccagagcg 1741 tggttgctga ccagttgaaa ggtttgaaga catacctgat atcgggtcag agagccaagg 1801 agtctcgctg cagctgtgca gaggccttgc tttctggctt tgaggtcatt gacggctcac 1861 aggtgtcctc aggccctagg ggacagggga cagcgtcatc agggaatgtc agtgacttgg 1921 cgcagactgt caaaaccttt gataacctta agacctgccc ttcgtgcgga atcacatttg 1981 ctcccaaatc tgaagccggc gccgagggag gagcacctca aaacggctgc caagacgagc 2041 ataaaaacag caccaaggct tctggaggac ctaatcccaa aactcagaac gggctcctca 2101 gccctcccca agaggagaag ctcaccaaca gtcagacttc tctgtgtgag atcttgcagg 2161 agaagggaag gtgggcaggg gtgagcctgg accagtcggc tctccttccg ctgaggttca 2221 agaacatccg ggagaaaacg gacgcccact ttgtggacgt tatcaaagaa gacagcctga 2281 tgaaagatta cttttttaag ccgcccatta atcagttcag cctgaacttc ctggatcagg 2341 agctggagcg atcctacagg accagctatc aggaagaggt cataaagaac tcccccgtga 2401 agacgtttgc tagtcccacc ttcagctccc tcctggatgt gtttctgtcg accacagtgt 2461 ttctgacgct gtccaccacc tgcttcctga agtacgaggc ggccaccgtg cctcccccgc 2521 ccgccgccct ggcggtcttc agtgcagccc tgctgctgga ggtgctgtcc ctcgcggtgt 2581 ccatcaggat ggtgttcttc ctggaggacg tcatggcctg caccaagcgc ctgctggagt 2641 ggatcgccgg ctggctacca cgtcactgca tcggggccat cctggtgtcg cttcccgcac 2701 tggccgtcta ctcccatgtc acctccgaat atgagaccaa catacacttc ccagtgttca 2761 caggctcggc cgcgctgatt gccgtcgtgc actactgtaa cttctgccag ctcagctcct 2821 ggatgaggtc ctccctcgcc accgtcgtgg gggccgggcc gctgctcctg ctctacgtct 2881 ccctgtgccc agacagttct gtattaactt cgccccttga cgcagtacag aatttcagtt 2941 ccgagaggaa cccgtgcaat agctcggtgc cgcgtgacct ccggcggccc gccagcctca 3001 tcggccagga ggtggttctc gtcttctttc tcctgctctt gttggtctgg ttcctgaatc 3061 gcgaatttga agtcagctac cgcctccact accacggaga cgtggaagcg gatcttcacc 3121 gcaccaagat ccagagcatg cgggaccagg cagactggct gctgaggaac atcatcccct 3181 accacgtggc tgagcagctg aaggtgtccc agacctactc caagaaccac gacagcggag 3241 gggtgatctt cgccagcatc gtcaacttca gcgagttcta cgaggagaac tacgagggcg 3301 gcaaggagtg ctaccgggtc ctcaacgagc tcatcgggga ctttgacgag ctcctaagca 3361 agccggacta cagcagcatc gagaagatca agaccatcgg agccacgtac atggcggcgt 3421 cagggctgaa caccgcgcag gcccaggacg gcagccaccc gcaggagcac ctgcagatcc 3481 tgttcgagtt cgccaaggag atgatgcgcg tggtggacga cttcaacaac aacatgctgt 3541 ggttcaactt caagctccgc gtcggcttca accatgggcc cctcacggcc ggggtcatcg 3601 gcaccaccaa gctgctgtac gacatctggg gagacaccgt caacatcgcc agcaggatgg 3661 acaccaccgg cgtggagtgc cgcatccagg tgagcgaaga gagctaccgc gtcttgagca 3721 agatgggcta tgacttcgac tacagaggga ccgtgaatgt caagggGAAA GGCCAGATGA 3781 AGACCTACCT GTACCCAAAG TGCACGGATC ACAGGGTCAT CCCACAGCAC CAGCTGTCCA 3841 TCTCCCCAGA CATCCGCGTC CAGGTGGATG GCAGCATCGG ACGGTCTCCC ACAGACGAGA 3901 TTGCCAACCT GGTGCCTTCT GTCCAGTATG TGGACAAGAC ATCTCTGGGT TCTGACAGCA 3961 GCACGCAGGC CAAGGATGCC CACCTGTCCC CCAAGAGACC GTGGAAGGAG CCCGTCAAAG 4021 CCGAAGAAAG GGGTCGATTT GGCAAAGCCA TAGAGAAAGA CGACTGTGAC GAAACAGGAA 4081 TAGAAGAAGC CAACGAACTC ACCAAGCTCA ACGTTTCAAA GAGTGTGTTG CCAACTTTCT 4141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 4201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 4261 GTGGAAAGGA CGAAAAAGGG TTAAATCTTG TTTCGACACG CGTTAAGTCg acaatcaacc 4321 tctggattac aaaatttgtg aaagatt