Construct: ORF TRCN0000475792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008928.1_s317c1
- Derived from:
- ccsbBroadEn_07239
- DNA Barcode:
- AGCCTGACAGCACTTAAATTCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSMAF (8439)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8439 | NSMAF | neutral sphingomyelinase ac... | NM_003580.4 | 99.9% | 100% | 1428C>T |
| 2 | human | 8439 | NSMAF | neutral sphingomyelinase ac... | NM_001144772.1 | 95.9% | 94.9% | (many diffs) |
| 3 | mouse | 18201 | Nsmaf | neutral sphingomyelinase (N... | NM_010945.2 | 87.3% | 91.7% | (many diffs) |
| 4 | mouse | 18201 | Nsmaf | neutral sphingomyelinase (N... | XM_006537664.2 | 86.7% | 91% | (many diffs) |
| 5 | mouse | 18201 | Nsmaf | neutral sphingomyelinase (N... | XM_006537663.2 | 84.6% | 88.9% | (many diffs) |
| 6 | mouse | 18201 | Nsmaf | neutral sphingomyelinase (N... | XM_006537662.3 | 84.1% | 88.2% | (many diffs) |
| 7 | mouse | 18201 | Nsmaf | neutral sphingomyelinase (N... | XM_011249943.2 | 71.4% | 75% | (many diffs) |
| 8 | mouse | 18201 | Nsmaf | neutral sphingomyelinase (N... | XM_011249944.2 | 71% | 75% | (many diffs) |
| 9 | mouse | 18201 | Nsmaf | neutral sphingomyelinase (N... | XR_390314.3 | 59.9% | (many diffs) | |
| 10 | mouse | 18201 | Nsmaf | neutral sphingomyelinase (N... | XR_001784107.1 | 51.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2817
- ORF length:
- 2751
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtttatccgg aagaagcagc aggagcagca gctgcagctc tactccaagg 121 agagattttc cttgctgctg cttaacttgg aggagtacta ctttgaacag catagagcca 181 atcacatttt gcacaagggc agtcaccatg aaaggaaaat cagaggctcc ttaaaaatat 241 gttcaaaatc ggtgattttt gaaccagatt caatatccca gcccatcatc aagattcctt 301 tgagagactg tataaaaata ggaaagcatg gagaaaatgg agccaataga cacttcacaa 361 aggcaaaatc tgggggtatt tcactcattt tcagtcaggt atatttcatt aaagaacata 421 atgttgttgc accatataaa atagaaaggg gcaaaatgga atatgttttt gaattggatg 481 ttcccgggaa agtggaagat gttgtggaga cgttgcttca gcttcacaga gcatcctgcc 541 ttgacaaatt gggtgaccaa accgccatga taacagctat tttgcagtct cgtttagcta 601 gaacatcatt tgacaaaaac aggttccaaa acatttctga aaagctgcac atggaatgca 661 aagcagaaat ggtgacgcct ctggtgacta atcctggaca cgtgtgcatc acggacacaa 721 acctgtattt tcagcccctc aacggctacc cgaaacctgt ggtccagata acactccaag 781 atgtccgccg catctacaaa aggaggcacg gcctcatgcc tctgggcttg gaagtatttt 841 gcacagaaga tgatctgtgt tccgacatct acctaaagtt ctatgaacct caagatagag 901 atgatctcta tttttacatt gccacatacc tagagcacca tgtggcggag cacactgctg 961 agagctacat gctgcagtgg cagcgtggac acctttccaa ctatcagtac ctccttcacc 1021 tcaacaacct ggccgaccgc agctgcaacg acctctccca gtaccctgtg tttccatgga 1081 taatacatga ttattccagc tcagaactag atttgtcaaa tccaggaacc ttccgggatc 1141 tcagtaagcc agtaggggcc ctaaataagg aacggctgga gagactactg acacgctacc 1201 aggaaatgcc tgaaccaaag ttcatgtatg ggagtcacta ctcttccccg ggttatgtac 1261 ttttttatct tgttaggatt gcaccagagt atatgctgtg cctgcagaat ggaagatttg 1321 ataatgcaga tagaatgttc aacagtattg cagaaacttg gaaaaactgt ctggatggtg 1381 caacggattt taaagagtta attccagaat tctatggtga tgatgtgagc tttctagtca 1441 atagcctgaa gttggatttg ggaaagagac aaggaggaca gatggttgac gatgtggagc 1501 ttcccccttg ggcttccagt cccgaggact ttctccagaa gagcaaagat gcattggaaa 1561 gcaattatgt gtctgaacac cttcacgagt ggattgatct aatatttggc tacaaacaaa 1621 aagggagtga tgcagttggg gcccataatg tatttcatcc cctgacctat gaaggaggtg 1681 tagacttgaa cagcatccag gatcctgatg agaaggtagc catgcttacg caaatcttgg 1741 aatttgggca gacaccaaaa caactatttg tgacaccaca tcctcgaagg atcaccccaa 1801 agtttaaaag tttgtcccag acctccagtt ataatgcttc tatggcagat tccccaggtg 1861 aagagtcttt tgaagacctg accgaagaaa gcaaaacact ggcctggaat aacatcacca 1921 aactgcagtt acacgagcac tataaaatcc acaaagaagc agttactgga atcacggtct 1981 ctcgcaatgg atcttcagta ttcacaacat cccaagattc caccttgaag atgttttcta 2041 aagaatcaaa aatgctacaa agaagtatat cattttcaaa tatggcttta tcgtcttgtt 2101 tacttttacc aggagatgcc actgtcataa cttcttcatg ggataataat gtctattttt 2161 attccatagc atttggaaga cgccaggaca cgttaatggg acatgatgat gctgttagta 2221 agatctgttg gcatgacaac aggctatatt ctgcatcgtg ggactctaca gtgaaggtgt 2281 ggtctggtgt tcctgcagag atgccaggca ccaaaagaca ccactttgac ttgctggccg 2341 agctggaaca tgatgtcagt gtagatacaa tcagtttaaa tgctgcaagc acactgttag 2401 tttccggcac caaagaaggc acagtgaata tttgggacct cacaacggcc accttaatgc 2461 accagattcc atgccattca gggattgtat gtgacactgc ttttagccca gatagtcgcc 2521 atgtccTCAG CACAGGAACA GATGGCTGTC TTAATGTCAT TGATGTGCAG ACAGGAATGC 2581 TCATCTCCTC CATGACATCA GATGAGCCCC AGAGGTGCTT TGTCTGGGAT GGAAATTCCG 2641 TTTTATCTGG CAGTCAGTCT GGTGAACTGC TCGTTTGGGA CCTCCTTGGA GCAAAAATCA 2701 GTGAGAGAAT ACAGGGCCAC ACAGGTGCTG TGACATGTAT ATGGATGAAT GAACAGTGTA 2761 GCAGTATCAT CACAGGAGGG GAAGACAGAC AAATTATATT CTGGAAATTG CAGTATTACC 2821 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2881 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2941 ATATATCTTG TGGAAAGGAC GAAGCCTGAC AGCACTTAAA TTCTAAACGC GTTAAGTCga 3001 caatcaacct ctggattaca aaatttgtga aagatt