Construct: ORF TRCN0000475805
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003824.1_s317c1
- Derived from:
- ccsbBroadEn_07868
- DNA Barcode:
- ATCTCTAAAAAGATGCATGTATGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAN2B2 (23324)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475805
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23324 | MAN2B2 | mannosidase alpha class 2B ... | NM_015274.3 | 99.7% | (many diffs) | |
| 2 | human | 23324 | MAN2B2 | mannosidase alpha class 2B ... | NM_001292038.2 | 94.7% | (many diffs) | |
| 3 | human | 23324 | MAN2B2 | mannosidase alpha class 2B ... | XR_241647.4 | 91.4% | (many diffs) | |
| 4 | human | 23324 | MAN2B2 | mannosidase alpha class 2B ... | XR_001741189.1 | 69.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 75
- ORF length:
- 6
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggtagctg tgctggctgc cgctgctggc accgctcctg ttgctgcgac 121 cgccaggggt ccagtccgcc ggccccatcc gggccttcgt ggtgccccac agccacatgg 181 acgtgggctg ggtctacact gtgcaggaaa gcatgcgggc gtacgccgcc aatgtctaca 241 cctcagtggt ggaagagctg gcccgcggcc agcagcgccg gttcatcgct gtggagcagg 301 agtttttccg gctgtggtgg gatggcgtcg cctcggacca gcagaaatac caggtccgcc 361 agctcctgga ggaaggacgc ctggaatttg tcatcggagg ccaggtcatg catgacgagg 421 ctgtgacgca ccttgatgac cagatcctgc agctcacaga aggacacggg tttctctatg 481 aaacatttgg gatccggcca cagttctcct ggcacgttga cccgtttggc gcctctgcca 541 cgacgcccac cctatttgcg ctggcgggct tcaatgccca cctcggctcc cggatcgact 601 acgacctgaa ggcagccatg caggaggccc gggggctgca gttcgtgtgg cgagggtccc 661 catccctctc agagcggcag gaaatcttca cgcacatcat ggaccagtac agctactgca 721 ccccgtccca catccctttc tccaacaggt caggatttta ctggaatggc gtggctgtct 781 tccccaagcc tcccccagat ggggtgtacc ccaacatgag tgagcctgtc accccagcca 841 acatcaacct ctatgccgag gccctggtgg ccaacgtgaa gcagagggcc gcctggttcc 901 ggacaccgca cgtcctctgg ccctggggat gtgacaagca gttcttcaat gcctcggtgc 961 agtttgccaa catggacccg ctgctggacc acatcaacag ccatgctgcc gagctcggtg 1021 tctcggtgca gtatgccacg ctgggcgact acttccgtgc cctgcacgct ctcaatgtca 1081 cctggcgtgt ccgcgaccac cacgacttcc tgccctattc cacagaacca ttccaggcct 1141 ggacgggctt ctacacgtcc cgcagctcac tgaaggggct ggcccggcga gccagcgcct 1201 tgttgtatgc cggggagtcc atgttcacac gctacctgtg gccggccccc cgtgggcatc 1261 tggaccccac ctgggccctg cagcagctcc agcagcttcg ctgggccgtc tccgaggtcc 1321 agcaccatga tgccatcact gggactgagt cccccaaggt gagagacatg tacgcaacgc 1381 acctggcctc ggggatgctg ggcgtgcgca agctgatggc ctccatcgtc ctagatgagc 1441 tccagcccca ggcacccatg gcggccagct ccgatgcagg acctgcagga cattttgcct 1501 cggtctacaa cccgctggcc tggacggtca ccaccatcgt caccctgact gttggtttcc 1561 ctggagtccg cgtcacagat gaggcgggcc acccagtgcc ctcgcagatc cagaactcaa 1621 cagagacccc atctgcgtat gacctgctta ttctgaccac aatcccaggc ctcagttacc 1681 ggcactacag catcagaccc actgcagggg cccaagaggg cacccaggag ccggctgcca 1741 ctgtggcgag cacccttcaa tttggccgca ggctgaggag acgcaccagc catgcgggca 1801 ggtacttggt gcctgtggca aacgactgct acattgtgct gctcgaccag gataccaacc 1861 tgatgcacag catctgggag agacagagta accgaacggt gcgcgtgacc caggaattcc 1921 tggagtacca cgtcaacagg gatgtgaaac agggccccat ttccgataac tacctgttca 1981 caccgggcaa ggccgcggtg cctgcgtggg aagctgtgga aatggagatt gtggcgggac 2041 agcttgtgac tgagatccgg cagtacttct acaggaacat gacagcacag aattacacgt 2101 atgcaatccg ctcccggctc acccatgtgc cgcagggcca tgacggggag ctgctctgcc 2161 accggataga gcaggagtac caagccggcc ccctggagct gaaccgtgag gctgtcctga 2221 ggaccagcac caacctaaac agccagcagg tcatctactc agacaacaac ggctaccaga 2281 tgcagcggag gccctacgtt tcctatgtga acaacagcat cgcccggaat tactacccca 2341 tggttcagtc ggccttcatg gaggatggca aaagcaggct tgtgttgctg tcggagcggg 2401 cacatggcat ctccagccaa gggaatgggc aggtggaggt catgctccac cggcggctgt 2461 ggaacaactt cgactgggac ctgggctaca acctcacgct gaacgacacc tcagtcgtcc 2521 acccagtgct ctggcttctg ctgggatcct ggtccctcac cactgccctg cgccagagga 2581 gcgcactggc gctgcagcac aggcccgtgg tgctgttcgg agacctcgct gggactgcgc 2641 cgaagctccc aggaccccag cagcaagagg ccgtgacgct gcccccgaat cttcacctgc 2701 agatcctgag catccctggc tggcgctaca gctccaacca cacggagcac tctcagaatc 2761 tccggaaagg ccatcgaggg gaagcccagg ctgaccTCCG CCGTGTCCTG CTGCGGCTCT 2821 ACCACCTGTA TGAAGTGGGC GAGGACCCAG TCCTGTCTCA GCCAGTAACA GTGAATCTGG 2881 AGGCTGTGCT GCAGGCGCTG GGGTCCGTGG TGGCAGTGGA GGAGCGCTCG CTCACAGGGA 2941 CCTGGGATTT GAGCATGCTG CACCGCTGGA GCTGGAGGAC GGGGCCTGGC CGCCACAGAG 3001 GTGACACCAC CTCTCCCTCG AGGCCACCAG GAGGCCCCAT CATCACCGTC CACCCAAAGG 3061 AAATCCGGAC GTTCTTTATT CACTTTCAAC AGCAGTTGCC AACTTTCTTG TACAAAGTGG 3121 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3181 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3241 AATCTCTAAA AAGATGCATG TATGAACGCG TTAAGTCgac aatcaacctc tggattacaa 3301 aatttgtgaa agatt