Construct: ORF TRCN0000475805

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003824.1_s317c1
Derived from:
ccsbBroadEn_07868
DNA Barcode:
ATCTCTAAAAAGATGCATGTATGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAN2B2 (23324)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475805
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23324 MAN2B2 mannosidase alpha class 2B ... NM_015274.3 99.7% (many diffs)
2 human 23324 MAN2B2 mannosidase alpha class 2B ... NM_001292038.2 94.7% (many diffs)
3 human 23324 MAN2B2 mannosidase alpha class 2B ... XR_241647.4 91.4% (many diffs)
4 human 23324 MAN2B2 mannosidase alpha class 2B ... XR_001741189.1 69.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
75
ORF length:
6
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggggtagctg tgctggctgc cgctgctggc accgctcctg ttgctgcgac
121cgccaggggt ccagtccgcc ggccccatcc gggccttcgt ggtgccccac agccacatgg
181acgtgggctg ggtctacact gtgcaggaaa gcatgcgggc gtacgccgcc aatgtctaca
241cctcagtggt ggaagagctg gcccgcggcc agcagcgccg gttcatcgct gtggagcagg
301agtttttccg gctgtggtgg gatggcgtcg cctcggacca gcagaaatac caggtccgcc
361agctcctgga ggaaggacgc ctggaatttg tcatcggagg ccaggtcatg catgacgagg
421ctgtgacgca ccttgatgac cagatcctgc agctcacaga aggacacggg tttctctatg
481aaacatttgg gatccggcca cagttctcct ggcacgttga cccgtttggc gcctctgcca
541cgacgcccac cctatttgcg ctggcgggct tcaatgccca cctcggctcc cggatcgact
601acgacctgaa ggcagccatg caggaggccc gggggctgca gttcgtgtgg cgagggtccc
661catccctctc agagcggcag gaaatcttca cgcacatcat ggaccagtac agctactgca
721ccccgtccca catccctttc tccaacaggt caggatttta ctggaatggc gtggctgtct
781tccccaagcc tcccccagat ggggtgtacc ccaacatgag tgagcctgtc accccagcca
841acatcaacct ctatgccgag gccctggtgg ccaacgtgaa gcagagggcc gcctggttcc
901ggacaccgca cgtcctctgg ccctggggat gtgacaagca gttcttcaat gcctcggtgc
961agtttgccaa catggacccg ctgctggacc acatcaacag ccatgctgcc gagctcggtg
1021tctcggtgca gtatgccacg ctgggcgact acttccgtgc cctgcacgct ctcaatgtca
1081cctggcgtgt ccgcgaccac cacgacttcc tgccctattc cacagaacca ttccaggcct
1141ggacgggctt ctacacgtcc cgcagctcac tgaaggggct ggcccggcga gccagcgcct
1201tgttgtatgc cggggagtcc atgttcacac gctacctgtg gccggccccc cgtgggcatc
1261tggaccccac ctgggccctg cagcagctcc agcagcttcg ctgggccgtc tccgaggtcc
1321agcaccatga tgccatcact gggactgagt cccccaaggt gagagacatg tacgcaacgc
1381acctggcctc ggggatgctg ggcgtgcgca agctgatggc ctccatcgtc ctagatgagc
1441tccagcccca ggcacccatg gcggccagct ccgatgcagg acctgcagga cattttgcct
1501cggtctacaa cccgctggcc tggacggtca ccaccatcgt caccctgact gttggtttcc
1561ctggagtccg cgtcacagat gaggcgggcc acccagtgcc ctcgcagatc cagaactcaa
1621cagagacccc atctgcgtat gacctgctta ttctgaccac aatcccaggc ctcagttacc
1681ggcactacag catcagaccc actgcagggg cccaagaggg cacccaggag ccggctgcca
1741ctgtggcgag cacccttcaa tttggccgca ggctgaggag acgcaccagc catgcgggca
1801ggtacttggt gcctgtggca aacgactgct acattgtgct gctcgaccag gataccaacc
1861tgatgcacag catctgggag agacagagta accgaacggt gcgcgtgacc caggaattcc
1921tggagtacca cgtcaacagg gatgtgaaac agggccccat ttccgataac tacctgttca
1981caccgggcaa ggccgcggtg cctgcgtggg aagctgtgga aatggagatt gtggcgggac
2041agcttgtgac tgagatccgg cagtacttct acaggaacat gacagcacag aattacacgt
2101atgcaatccg ctcccggctc acccatgtgc cgcagggcca tgacggggag ctgctctgcc
2161accggataga gcaggagtac caagccggcc ccctggagct gaaccgtgag gctgtcctga
2221ggaccagcac caacctaaac agccagcagg tcatctactc agacaacaac ggctaccaga
2281tgcagcggag gccctacgtt tcctatgtga acaacagcat cgcccggaat tactacccca
2341tggttcagtc ggccttcatg gaggatggca aaagcaggct tgtgttgctg tcggagcggg
2401cacatggcat ctccagccaa gggaatgggc aggtggaggt catgctccac cggcggctgt
2461ggaacaactt cgactgggac ctgggctaca acctcacgct gaacgacacc tcagtcgtcc
2521acccagtgct ctggcttctg ctgggatcct ggtccctcac cactgccctg cgccagagga
2581gcgcactggc gctgcagcac aggcccgtgg tgctgttcgg agacctcgct gggactgcgc
2641cgaagctccc aggaccccag cagcaagagg ccgtgacgct gcccccgaat cttcacctgc
2701agatcctgag catccctggc tggcgctaca gctccaacca cacggagcac tctcagaatc
2761tccggaaagg ccatcgaggg gaagcccagg ctgaccTCCG CCGTGTCCTG CTGCGGCTCT
2821ACCACCTGTA TGAAGTGGGC GAGGACCCAG TCCTGTCTCA GCCAGTAACA GTGAATCTGG
2881AGGCTGTGCT GCAGGCGCTG GGGTCCGTGG TGGCAGTGGA GGAGCGCTCG CTCACAGGGA
2941CCTGGGATTT GAGCATGCTG CACCGCTGGA GCTGGAGGAC GGGGCCTGGC CGCCACAGAG
3001GTGACACCAC CTCTCCCTCG AGGCCACCAG GAGGCCCCAT CATCACCGTC CACCCAAAGG
3061AAATCCGGAC GTTCTTTATT CACTTTCAAC AGCAGTTGCC AACTTTCTTG TACAAAGTGG
3121TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
3181CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
3241AATCTCTAAA AAGATGCATG TATGAACGCG TTAAGTCgac aatcaacctc tggattacaa
3301aatttgtgaa agatt

Download FASTA (ORF) (Full)