Transcript: Human XR_241647.4

PREDICTED: Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAN2B2 (23324)
Length:
3037
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_241647.4
NBCI Gene record:
MAN2B2 (23324)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_241647.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000372959 TGGCGAGCACCCTTCAATTTG pLKO_005 1698 3UTR 100% 13.200 18.480 N MAN2B2 n/a
2 TRCN0000372899 CTGCGTATGACCTGCTTATTC pLKO_005 1587 3UTR 100% 13.200 9.240 N MAN2B2 n/a
3 TRCN0000049635 CCAGCCAACATCAACCTCTAT pLKO.1 788 3UTR 100% 4.950 3.465 N MAN2B2 n/a
4 TRCN0000049636 GCCCTACGTTTCCTATGTGAA pLKO.1 2245 3UTR 100% 4.950 3.465 N MAN2B2 n/a
5 TRCN0000049637 GCAGGAAATCTTCACGCACAT pLKO.1 631 3UTR 100% 4.050 2.835 N MAN2B2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_241647.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07868 pDONR223 100% 91.4% None (many diffs) n/a
2 ccsbBroad304_07868 pLX_304 0% 91.4% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000475805 ATCTCTAAAAAGATGCATGTATGA pLX_317 10.1% 91.4% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV