Construct: ORF TRCN0000475819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011914.1_s317c1
- Derived from:
- ccsbBroadEn_02784
- DNA Barcode:
- ATTAACTTCATACCCCGTATCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUZ12 (23512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23512 | SUZ12 | SUZ12 polycomb repressive c... | NM_015355.4 | 100% | 100% | |
| 2 | human | 23512 | SUZ12 | SUZ12 polycomb repressive c... | NM_001321207.2 | 96.8% | 96.8% | 384_385ins69 |
| 3 | human | 23512 | SUZ12 | SUZ12 polycomb repressive c... | XM_006721794.3 | 81.5% | 81.5% | 0_1ins408 |
| 4 | human | 23512 | SUZ12 | SUZ12 polycomb repressive c... | XM_017024409.1 | 59.6% | 59.6% | 0_1ins894 |
| 5 | human | 23512 | SUZ12 | SUZ12 polycomb repressive c... | XR_001752462.1 | 47.2% | 1_208del;1123_1124ins106;2320_4359del | |
| 6 | human | 440423 | SUZ12P1 | SUZ12 pseudogene 1 | NR_144393.1 | 33.5% | (many diffs) | |
| 7 | mouse | 52615 | Suz12 | suppressor of zeste 12 homo... | NM_199196.2 | 92.8% | 96.8% | (many diffs) |
| 8 | mouse | 52615 | Suz12 | suppressor of zeste 12 homo... | NM_001163018.1 | 89.7% | 93.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2283
- ORF length:
- 2217
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcctcagaag cacggcggtg ggggaggggg cggctcgggg cccagcgcgg 121 ggtccggggg aggcggcttc gggggttcgg cggcggtggc ggcggcgacg gcttcgggcg 181 gcaaatccgg cggcgggagc tgtggagggg gtggcagtta ctcggcctcc tcctcctcct 241 ccgcggcggc agcggcgggg gctgcggtgt taccggtgaa gaagccgaaa atggagcacg 301 tccaggctga ccacgagctt ttcctccagg cctttgagaa gccaacacag atctatagat 361 ttcttcgaac tcggaatctc atagcaccaa tatttttgca cagaactctt acttacatgt 421 ctcatcgaaa ctccagaaca aacatcaaaa ggaaaacatt taaagttgat gatatgttat 481 caaaagtaga gaaaatgaaa ggagagcaag aatctcatag cttgtcagct catttgcagc 541 ttacgtttac tggtttcttc cacaaaaatg ataagccatc accaaactca gaaaatgaac 601 aaaattctgt taccctggaa gtcctgcttg tgaaagtttg ccacaaaaaa agaaaggatg 661 taagttgtcc aataaggcaa gttcccacag gtaaaaagca ggtgcctttg aatcctgacc 721 tcaatcaaac aaaacccgga aatttcccgt cccttgcagt ttccagtaat gaatttgaac 781 ctagtaacag ccatatggtg aagtcttact cgttgctatt tagagtgact cgtccaggaa 841 gaagagagtt taatggaatg attaatggag aaaccaatga aaatattgat gtcaatgaag 901 agcttccagc cagaagaaaa cgaaatcgtg aggatgggga aaagacattt gttgcacaaa 961 tgacagtatt tgataaaaac aggcgcttac agcttttaga tggggaatat gaagtagcca 1021 tgcaggaaat ggaagaatgt ccaataagca agaaaagagc aacatgggag actattcttg 1081 atgggaagag gctgcctcca ttcgaaacat tttctcaggg acctacgttg cagttcactc 1141 ttcgttggac aggagagacc aatgataaat ctacggctcc tattgccaaa cctcttgcca 1201 ctagaaattc agagagtctc catcaggaaa acaagcctgg ttcagttaaa cctactcaaa 1261 ctattgctgt taaagaatca ttgactacag atctacaaac aagaaaagaa aaggatactc 1321 caaatgaaaa ccgacaaaaa ttaagaatat tttatcagtt tctctataac aacaatacaa 1381 ggcaacaaac tgaagcaaga gatgacctgc attgcccttg gtgtactctg aactgccgca 1441 aactttatag tttactcaag catcttaaac tctgccatag cagatttatc ttcaactatg 1501 tttatcatcc aaaaggtgct aggatagatg tttctatcaa tgagtgttat gatggctcct 1561 atgcaggaaa tcctcaggat attcatcgcc aacctggatt tgcttttagt cgcaacggac 1621 cagttaagag aacacctatc acacatattc ttgtgtgcag gccaaaacga acaaaagcaa 1681 gcatgtctga atttcttgaa tctgaagatg gggaagtaga acagcaaaga acatatagta 1741 gtggccacaa tcgtctgtat ttccatagtg atacctgctt acctctccgt ccacaagaaa 1801 tggaagtaga tagtgaagat gaaaaggatc ctgaatggct aagagaaaaa accattacac 1861 aaattgaaga gttttctgat gttaatgaag gagagaaaga agtgatgaaa ctctggaatc 1921 tccatgtcat gaagcatggg tttattgctg acaatcaaat gaatCATGCC TGTATGCTGT 1981 TTGTAGAAAA TTATGGACAG AAAATAATTA AGAAGAATTT ATGTCGAAAC TTCATGCTTC 2041 ATCTAGTCAG CATGCATGAC TTTAATCTTA TTAGCATAAT GTCAATAGAT AAAGCTGTTA 2101 CCAAGCTCCG TGAAATGCAG CAAAAATTAG AAAAGGGGGA ATCTGCTTCC CCTGCAAACG 2161 AAGAAATAAC TGAAGAACAA AATGGGACAG CAAATGGATT TAGTGAAATT AACTCAAAAG 2221 AGAAAGCTTT GGAAACAGAT AGTGTCTCAG GGGTTTCAAA ACAGAGCAAA AAACAAAAAC 2281 TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2341 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2401 GGCTTTATAT ATCTTGTGGA AAGGACGAAT TAACTTCATA CCCCGTATCC TTACGCGTTA 2461 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt