Construct: ORF TRCN0000475836
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003233.1_s317c1
- Derived from:
- ccsbBroadEn_04216
- DNA Barcode:
- AATGCGTGCGCAGGACCCTAGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PBX4 (80714)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475836
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80714 | PBX4 | PBX homeobox 4 | NM_025245.3 | 100% | 100% | |
| 2 | human | 80714 | PBX4 | PBX homeobox 4 | XM_011528320.3 | 95.6% | 95.3% | 194_244del |
| 3 | human | 80714 | PBX4 | PBX homeobox 4 | XM_017027326.1 | 86.7% | 86.1% | (many diffs) |
| 4 | human | 80714 | PBX4 | PBX homeobox 4 | XM_017027327.1 | 83.3% | 83.1% | 0_1ins144;50_100del |
| 5 | human | 80714 | PBX4 | PBX homeobox 4 | XR_001753767.2 | 79.1% | (many diffs) | |
| 6 | human | 80714 | PBX4 | PBX homeobox 4 | XM_006722911.3 | 77% | 77% | 0_1ins258 |
| 7 | human | 80714 | PBX4 | PBX homeobox 4 | XM_011528322.2 | 77% | 77% | 0_1ins258 |
| 8 | human | 80714 | PBX4 | PBX homeobox 4 | NR_038198.2 | 68.2% | 1_58del;395_396ins101;1080_1394del | |
| 9 | human | 80714 | PBX4 | PBX homeobox 4 | XM_017027329.2 | 55.8% | 55.8% | 0_1ins495 |
| 10 | human | 80714 | PBX4 | PBX homeobox 4 | XM_017027330.2 | 36.4% | 31.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1191
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgccccg ccgcgccccg cgccatcgcc ccccgccccg cggcgcctcg 121 acacgagcga cgtcctgcag cagatcatgg ccatcaccga ccagagcctg gacgaggcac 181 aggccagaaa gcatgctctg aattgccatc ggatgaagcc tgctctgttc agcgtgctct 241 gtgagatcaa ggaaaagaca gtggtaagca tccgtggcat tcaagacgaa gatccccctg 301 acgcccagct cctgaggctg gataacatgc tgctggctga gggcgtgtgc aggcccgaga 361 agagaggaag aggaggagcg gtggccaggg ccggcacagc aacaccaggt ggctgtccaa 421 atgacaatag cattgagcac tctgactaca gggccaagct gtcccagatc cgacagattt 481 accactctga gctagagaaa tatgaacagg cctgtcgtga gttcaccacg cacgtcacca 541 acctcctcca ggagcagagc aggatgaggc ctgtctcccc taaggagatt gagcgcatgg 601 tcggcgccat tcacggcaag ttcagcgcca tccagatgca gttgaagcag agcacctgtg 661 aggcagtgat gaccctgcgt tcgcggctgc tcgatgccag gcgcaagcgg cggaatttca 721 gcaagcaggc gacggaagtg ctgaatgagt atttttactc ccatctgaac aacccttacc 781 ccagcgaaga agccaaagaa gagctggcca ggaagggcgg cctcaccatc tcccaggtct 841 ctaactggtt tGGCAACAAA AGAATCCGGT ATAAAAAGAA CATGGGGAAG TTTCAAGAAG 901 AGGCTACCAT TTACACGGGT AAAACGGCTG TGGATACCAC GGAAGTTGGG GTCCCAGGGA 961 ACCACGCCAG CTGCCTGTCA ACACCTAGCT CCGGCTCCTC TGGACCCTTC CCGCTGCCCA 1021 GCGCTGGGGA CGCCTTCCTC ACCCTGCGGA CTCTGGCCTC TCTCCAGCCT CCTCCTGGGG 1081 GAGGCTGCCT GCAGTCCCAG GCCCAGGGTA GCTGGCAGGG GGCCACCCCC CAACCTGCAA 1141 CTGCCTCACC TGCTGGAGAC CCTGGCAGCA TCAACTCCAG TACATCTAAT TTGCCAACTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGAAATG CGTGCGCAGG ACCCTAGGCA ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt