Construct: ORF TRCN0000475861
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012443.1_s317c1
- Derived from:
- ccsbBroadEn_05272
- DNA Barcode:
- ACTTAAAAACAATAAGAATTAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC35F1 (222553)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475861
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222553 | SLC35F1 | solute carrier family 35 me... | NM_001029858.4 | 100% | 100% | |
| 2 | human | 222553 | SLC35F1 | solute carrier family 35 me... | XM_005266865.4 | 100% | 100% | |
| 3 | mouse | 215085 | Slc35f1 | solute carrier family 35, m... | NM_178675.4 | 90.2% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1293
- ORF length:
- 1224
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatcccccct gagcagccgc agcagcagct gcagccgccg tcgccagccc 121 cgccgaacca tgtggtgacc accatcgaga acctgccggc cgagggcagc ggcggcggcg 181 ggagcctgtc cgcctcctcc cgggctggcg tgcgccagag gatccgcaaa gtgctgaaca 241 gggagatgtt aatctctgtg gccctaggcc aggtgttatc cctccttatt tgtggaattg 301 gcttgactag caagtatctg tcagaagatt tccacgccaa cacaccagtc ttccagagtt 361 tcctcaatta cattcttctc ttcttggtct ataccaccac actagccgtc agacaaggag 421 aagaaaacct cctggcaatt ttacgacgaa gatggtggaa gtacatgatt ttgggactca 481 tagacctgga agcaaattat ctggtggtca aggcttacca atacacaact ctgaccagta 541 tccagctcct ggactgtttt gtgatcccag tcgtgatttt gctctcctgg ttcttcctgc 601 tgatccggta caaggctgtg catttcatcg gcatcgttgt ctgcatcctg ggaatgggct 661 gcatggtggg agcagatgtg cttgtgggaa gacatcaggg agcaggggaa aataagctgg 721 taggggacct tctggtctta ggaggagcca cactctatgg tatttctaac gtctgggaag 781 aatacatcat ccgaactctg agccgagtgg aattcctggg aatgattggt ctctttggag 841 catttttcag tggaattcaa ttagctataa tggagcataa ggaactgttg aaggtgccct 901 gggactggca aataggactg ctcTACGTTG GCTTTAGTGC CTGCATGTTT GGTCTCTACA 961 GCTTTATGCC AGTCGTCATA AAGAAAACCA GTGCCACTTC AGTCAACCTC TCCTTGCTCA 1021 CAGCAGACTT GTACAGCCTG TTCTGTGGAT TGTTTCTCTT CCACTACAAG TTTTCAGGAC 1081 TTTATCTCCT GTCTTTCTTC ACCATCCTCA TTGGGCTGGT GCTCTACTCC TCCACCTCCA 1141 CCTACATAGC CCAGGACCCC CGAGTGTATA AGCAGTTCCG CAATCCTTCA GGACCTGTTG 1201 TGGACTTACC GACCACAGCT CAGGTGGAAC CCTCAGTCAC CTACACCAGC CTGGGCCAGG 1261 AGACCGAAGA GGAGCCTCAT GTTCGTGTGG CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1321 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1381 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 1441 TTAAAAACAA TAAGAATTAG CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1501 ttgtgaaaga tt