Construct: ORF TRCN0000475882
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000555.1_s317c1
- Derived from:
- ccsbBroadEn_09880
- DNA Barcode:
- CGCACCCCTGTGATTACCAATCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HS3ST5 (222537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475882
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222537 | HS3ST5 | heparan sulfate-glucosamine... | NM_153612.3 | 99.9% | 100% | 492G>A |
| 2 | human | 222537 | HS3ST5 | heparan sulfate-glucosamine... | XM_006715379.2 | 99.9% | 100% | 492G>A |
| 3 | human | 222537 | HS3ST5 | heparan sulfate-glucosamine... | XM_011535588.2 | 99.9% | 100% | 492G>A |
| 4 | human | 222537 | HS3ST5 | heparan sulfate-glucosamine... | XM_017010470.1 | 99.9% | 100% | 492G>A |
| 5 | human | 222537 | HS3ST5 | heparan sulfate-glucosamine... | XM_017010471.1 | 99.9% | 100% | 492G>A |
| 6 | human | 222537 | HS3ST5 | heparan sulfate-glucosamine... | XM_017010472.1 | 99.9% | 100% | 492G>A |
| 7 | human | 222537 | HS3ST5 | heparan sulfate-glucosamine... | XM_017010473.1 | 99.9% | 100% | 492G>A |
| 8 | human | 222537 | HS3ST5 | heparan sulfate-glucosamine... | XM_017010474.2 | 99.9% | 100% | 492G>A |
| 9 | mouse | 319415 | Hs3st5 | heparan sulfate (glucosamin... | NM_001081208.2 | 90% | 96.8% | (many diffs) |
| 10 | mouse | 319415 | Hs3st5 | heparan sulfate (glucosamin... | NM_001253355.1 | 90% | 96.8% | (many diffs) |
| 11 | mouse | 319415 | Hs3st5 | heparan sulfate (glucosamin... | NM_001253356.1 | 90% | 96.8% | (many diffs) |
| 12 | mouse | 319415 | Hs3st5 | heparan sulfate (glucosamin... | XM_006512761.2 | 90% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1107
- ORF length:
- 1038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctattcaaa cagcaggcgt ggctgagaca gaagctcctg gtgctgggaa 121 gccttgccgt tgggagtctc ctgtatctag tcgccagagt tgggagcttg gataggctac 181 aacccatttg ccccattgaa ggtcgactgg gtggagcccg cactcaggct gaattcccac 241 ttcgcgccct gcagtttaag cgtggcctgc tgcacgagtt ccggaagggc aacgcttcca 301 aggagcaggt tcgcctccat gacctggtcc agcagctccc caaggccatt atcattgggg 361 tgaggaaagg aggcacaagg gccctgcttg aaatgctgaa cctacatccg gcagtagtca 421 aagcctctca agaaatccac ttttttgata atgatgagaa ttatggtaag ggcattgagt 481 ggtataggaa aaagatgcct ttttcctacc ctcagcaaat cacaattgaa aagagcccag 541 catattttat cacagaggaa gttccagaaa ggatttacaa aatgaactca tccatcaagt 601 tgttgatcat tgtcagggag ccaaccacaa gagctatttc tgattatact caggtgctag 661 aggggaagga gaggaagaac aaaacttatt acaagtttga gaagctggcc atagacccTA 721 ATACATGCGA AGTGAACACA AAATACAAAG CAGTAAGAAC CAGCATCTAC ACCAAACATC 781 TGGAAAGGTG GTTGAAATAC TTTCCAATTG AGCAATTTCA TGTCGTCGAT GGAGATCGCC 841 TCATCACGGA ACCTCTGCCA GAACTTCAGC TCGTGGAGAA GTTCCTAAAT CTGCCTCCAA 901 GGATAAGTCA ATACAATTTA TACTTCAATG CTACCAGAGG GTTTTACTGC TTGCGGTTTA 961 ATATTATCTT TAATAAGTGC CTGGCGGGCA GCAAGGGGCG CATTCATCCA GAGGTGGACC 1021 CCTCTGTCAT TACTAAATTG CGCAAATTCT TTCATCCTTT TAATCAAAAA TTTTACCAGA 1081 TCACTGGGAG GACATTGAAC TGGCCCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGCACCCC 1261 TGTGATTACC AATCCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt