Construct: ORF TRCN0000475889

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009813.1_s317c1
Derived from:
ccsbBroadEn_13119
DNA Barcode:
AACCGAACTCAGTTATTAGGGGTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KDF1 (126695)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475889
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 126695 KDF1 keratinocyte differentiatio... NM_152365.3 61.3% 61.3% 1_462del
2 human 126695 KDF1 keratinocyte differentiatio... XM_005245735.2 61.3% 61.3% 1_462del
3 human 126695 KDF1 keratinocyte differentiatio... XM_011540622.2 61.3% 61.3% 1_462del
4 mouse 69073 Kdf1 keratinocyte differentiatio... NM_001083916.1 53.9% 57.5% (many diffs)
5 mouse 69073 Kdf1 keratinocyte differentiatio... NM_133707.2 53.9% 57.5% (many diffs)
6 mouse 69073 Kdf1 keratinocyte differentiatio... XM_006539150.3 53.9% 57.5% (many diffs)
7 mouse 69073 Kdf1 keratinocyte differentiatio... XM_006539151.3 53.9% 57.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
798
ORF length:
732
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg cagcagcttc agctaccccg atgttaagct caaaggcatc cctgtgtatc
121cctacccgag ggccacctcc ccagcccctg atgcggactc ctgctgcaag gagccactgg
181ccgatccccc acccatgcga cacagcctgc ccagcacctt tgccagtagt cctcgtggct
241ccgaggagta ctattctttc catgagtcgg acctggacct gccggagatg ggcagtggct
301ccatgtcgag ccgagaaatt gatgtgctca tcttcaagaa gctgacagag ctgttcagcg
361tacaccagat cgatgagctg gccaagtgca catcagacac tgtgttcctg gagaagacca
421gtaagatctc ggaccttatc agcagcatca cgcaggacta ccacctggat gagcaggatg
481ctgagggccg ccTGGTACGC GGCATCATTC GCATTAGTAC CCGAAAGAGC CGTGCTCGCC
541CACAGACCTC GGAGGGTCGT TCAACTCGGG CTGCTGCCCC AACCGCTGCT GCCCCTGACA
601GTGGCCATGA GACCATGGTG GGCTCAGGTC TCAGCCAGGA TGAGCTGACA GTGCAGATCT
661CCCAGGAGAC GACTGCAGAT GCCATCGCCC GGAAGCTGAG GCCTTATGGA GCTCCAGGGT
721ACCCAGCAAG CCATGACTCA TCCTTCCAGG GCACCGACAC AGACTCGTCG GGGGCACCCT
781TGCTCCAGGT GTACTGCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
841TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
901GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAACCGAA CTCAGTTATT
961AGGGGTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)