Construct: ORF TRCN0000475901
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006830.2_s317c1
- Derived from:
- ccsbBroadEn_14422
- DNA Barcode:
- AACTGCGCTTGTTTCCCTCCGCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DCLK2 (166614)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475901
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_017007832.2 | 96.4% | .5% | (many diffs) |
| 2 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_005262786.3 | 90.6% | .5% | (many diffs) |
| 3 | human | 166614 | DCLK2 | doublecortin like kinase 2 | NM_001040260.4 | 90.5% | .5% | (many diffs) |
| 4 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_005262785.3 | 88.7% | .5% | (many diffs) |
| 5 | human | 166614 | DCLK2 | doublecortin like kinase 2 | NM_001040261.5 | 88.5% | .5% | (many diffs) |
| 6 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_024453914.1 | 85% | .6% | 10_11insC;1776_1776delGins310 |
| 7 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XR_244621.3 | 82.1% | (many diffs) | |
| 8 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_024453915.1 | 79.2% | .6% | (many diffs) |
| 9 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_024453913.1 | 78.9% | .5% | (many diffs) |
| 10 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_017007833.2 | 77.2% | .5% | (many diffs) |
| 11 | human | 166614 | DCLK2 | doublecortin like kinase 2 | NR_036614.2 | 50.1% | 1_583del;593_594insC;2669_4155del | |
| 12 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502071.2 | 89.9% | .4% | (many diffs) |
| 13 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_017319718.1 | 87.9% | .4% | (many diffs) |
| 14 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502069.2 | 87.7% | .4% | (many diffs) |
| 15 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195499.1 | 86.9% | .4% | (many diffs) |
| 16 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195498.1 | 86.8% | .4% | (many diffs) |
| 17 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502074.2 | 84.2% | .4% | (many diffs) |
| 18 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502068.2 | 84.1% | .4% | (many diffs) |
| 19 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502072.2 | 84.1% | .4% | (many diffs) |
| 20 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502073.2 | 84% | .4% | (many diffs) |
| 21 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502075.2 | 83.7% | .4% | (many diffs) |
| 22 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195497.1 | 82.5% | .3% | (many diffs) |
| 23 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_027539.5 | 82.4% | .3% | (many diffs) |
| 24 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195496.1 | 80.8% | .3% | (many diffs) |
| 25 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502065.2 | 80.7% | .3% | (many diffs) |
| 26 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502070.2 | 80.7% | .4% | (many diffs) |
| 27 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502076.2 | 80.6% | .4% | (many diffs) |
| 28 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502077.2 | 80.5% | .4% | (many diffs) |
| 29 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502078.2 | 80.2% | .4% | (many diffs) |
| 30 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502067.2 | 79.1% | .4% | (many diffs) |
| 31 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_011240226.1 | 78% | .4% | (many diffs) |
| 32 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502066.2 | 77.5% | .4% | (many diffs) |
| 33 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_011240227.1 | 76.7% | .4% | (many diffs) |
| 34 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_017319719.1 | 76.4% | .4% | (many diffs) |
| 35 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195500.1 | 76% | .5% | (many diffs) |
| 36 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XR_867231.2 | 58% | (many diffs) | |
| 37 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XR_867230.2 | 51.5% | (many diffs) | |
| 38 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XR_001783745.1 | 45% | (many diffs) | |
| 39 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XR_867229.1 | 28.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 102
- ORF length:
- 36
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcacccag gagtatcgag ctggagcact ttgaggaacg ggacaaaagg 121 ccgcggccgg ggtcgcggag aggggccccc agctcctccg ggggcagcag cagctcgggc 181 cccaagggga acgggctcat ccccagtccg gcgcacagtg cccactgcag cttctaccgc 241 acgcggaccc tgcaggccct cagctcggag aagaaggcca agaaggcgcg cttctaccgg 301 aacggggacc gctacttcaa gggcctggtg tttgccatct ccagcgaccg cttccggtcc 361 ttcgatgcgc tcctcataga gctcacccgc tccctgtcgg acaacgtgaa cctgccccag 421 ggtgtccgca ctatctacac catcgacggc agccggaagg tcaccagcct ggacgagctg 481 ctggaaggtg agagttacgt gtgtgcatcc aatgaaccat ttcgtaaagt cgattacacc 541 aaaaatatta atccaaactg gtctgtgaac atcaagggtg ggacatcccg agcgctggct 601 gctgcctcct ctgtgaaaag tgaagtaaaa gaaagtaaag atttcatcaa acccaagtta 661 gtgactgtga ttcgaagtgg agtgaagcct agaaaagccg tgcggatcct tctgaataaa 721 aagactgctc attcctttga acaagtctta acagatatca ccgaagccat taaactagac 781 tcaggagtcg tcaagaggct ctgcaccctg gatggaaagc aggttacttg tctgcaagac 841 ttttttggtg atgacgatgt ttttattgca tgtggaccag aaaaatttcg ttatgcccaa 901 gatgactttg tcctggatca tagtgaatgt cgtgtcctga agtcatctta ttctcgatcc 961 tcagctgtta agtattctgg atccaaaagc cctgggccct ctcgacgcag caaatcacca 1021 gcttcagtta atggaactcc cagcagccaa ctttctactc ctaaatctac gaaatcctcc 1081 agttcctctc caactagtcc aggaagtttc agaggattaa agatttctgc tcatggcaga 1141 tcttcttcca atgtaaacgg tggacctgag cttgaccgtt gcataagtcc tgaaggtgtg 1201 aatggaaaca gatgctctga atcatcaact cttcttgaga aatacaaaat tggaaaggtc 1261 attggtgatg gcaattttgc agtagtcaaa gagtgtatag acaggtccac tggaaaggag 1321 tttgccctaa agattataga caaagccaaa tgttgtggaa aggaacacct gattgagaat 1381 gaagtgtcaa tactgcgccg agtgaaacat cccaatatca ttatgctggt cgaggagatg 1441 gaaacagcaa ctgagctctt tctggtgatg gaattggtca aaggtggaga tctctttgat 1501 gcaattactt cgtcgaccaa gtacactgag agagatggca gtgccatggt gtacaactta 1561 gccaatgccc tcaggtatct ccatggcctc agcatcgtgc acagagacat caaaccagag 1621 aatctcttgg tgtgtgaata tcctgatgga accaagtctt tgaaactggg agactttggg 1681 cttgcgactg tggtagaagg ccctttatac acagtctgtg gcacacccac ttatgtggct 1741 ccagaaatca ttgctgaaac tggctatggc ctgaaggtgg acatttgggc agctggtgtg 1801 atcacataca tacttctctg tggattccca ccattccgaa gtgagaacaa TCTCCAGGAA 1861 GATCTCTTCG ACCAGATCTT GGCTGGGAAG CTGGAGTTTC CGGCCCCCTA CTGGGATAAC 1921 ATCACGGACT CTGCCAAGGA ATTAATCAGT CAAATGCTTC AGGTAAATGT TGAAGCTCGG 1981 TGTACCGCGG GACAAATCCT GAGTCACCCC TGGGTGTCAG ATGATGCCTC CCAGGAGAAT 2041 AACATGCAAG CTGAGGTGAC AGGTAAACTA AAACAGCACT TTAATAATGC GCTCCCCAAA 2101 CAGAACAGCA CTACCACCGG GGTCTCCGTC ATCATGTTTG ATTTGACAGT TTGCCCAACT 2161 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 2221 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 2281 TCTTGTGGAA AGGACGAAAC TGCGCTTGTT TCCCTCCGCC AACGCGTTAA GTCgacaatc 2341 aacctctgga ttacaaaatt tgtgaaagat t