Construct: ORF TRCN0000475901

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006830.2_s317c1
Derived from:
ccsbBroadEn_14422
DNA Barcode:
AACTGCGCTTGTTTCCCTCCGCCA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DCLK2 (166614)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475901
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 166614 DCLK2 doublecortin like kinase 2 XM_017007832.2 96.4% .5% (many diffs)
2 human 166614 DCLK2 doublecortin like kinase 2 XM_005262786.3 90.6% .5% (many diffs)
3 human 166614 DCLK2 doublecortin like kinase 2 NM_001040260.4 90.5% .5% (many diffs)
4 human 166614 DCLK2 doublecortin like kinase 2 XM_005262785.3 88.7% .5% (many diffs)
5 human 166614 DCLK2 doublecortin like kinase 2 NM_001040261.5 88.5% .5% (many diffs)
6 human 166614 DCLK2 doublecortin like kinase 2 XM_024453914.1 85% .6% 10_11insC;1776_1776delGins310
7 human 166614 DCLK2 doublecortin like kinase 2 XR_244621.3 82.1% (many diffs)
8 human 166614 DCLK2 doublecortin like kinase 2 XM_024453915.1 79.2% .6% (many diffs)
9 human 166614 DCLK2 doublecortin like kinase 2 XM_024453913.1 78.9% .5% (many diffs)
10 human 166614 DCLK2 doublecortin like kinase 2 XM_017007833.2 77.2% .5% (many diffs)
11 human 166614 DCLK2 doublecortin like kinase 2 NR_036614.2 50.1% 1_583del;593_594insC;2669_4155del
12 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502071.2 89.9% .4% (many diffs)
13 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_017319718.1 87.9% .4% (many diffs)
14 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502069.2 87.7% .4% (many diffs)
15 mouse 70762 Dclk2 doublecortin-like kinase 2 NM_001195499.1 86.9% .4% (many diffs)
16 mouse 70762 Dclk2 doublecortin-like kinase 2 NM_001195498.1 86.8% .4% (many diffs)
17 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502074.2 84.2% .4% (many diffs)
18 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502068.2 84.1% .4% (many diffs)
19 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502072.2 84.1% .4% (many diffs)
20 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502073.2 84% .4% (many diffs)
21 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502075.2 83.7% .4% (many diffs)
22 mouse 70762 Dclk2 doublecortin-like kinase 2 NM_001195497.1 82.5% .3% (many diffs)
23 mouse 70762 Dclk2 doublecortin-like kinase 2 NM_027539.5 82.4% .3% (many diffs)
24 mouse 70762 Dclk2 doublecortin-like kinase 2 NM_001195496.1 80.8% .3% (many diffs)
25 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502065.2 80.7% .3% (many diffs)
26 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502070.2 80.7% .4% (many diffs)
27 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502076.2 80.6% .4% (many diffs)
28 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502077.2 80.5% .4% (many diffs)
29 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502078.2 80.2% .4% (many diffs)
30 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502067.2 79.1% .4% (many diffs)
31 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_011240226.1 78% .4% (many diffs)
32 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_006502066.2 77.5% .4% (many diffs)
33 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_011240227.1 76.7% .4% (many diffs)
34 mouse 70762 Dclk2 doublecortin-like kinase 2 XM_017319719.1 76.4% .4% (many diffs)
35 mouse 70762 Dclk2 doublecortin-like kinase 2 NM_001195500.1 76% .5% (many diffs)
36 mouse 70762 Dclk2 doublecortin-like kinase 2 XR_867231.2 58% (many diffs)
37 mouse 70762 Dclk2 doublecortin-like kinase 2 XR_867230.2 51.5% (many diffs)
38 mouse 70762 Dclk2 doublecortin-like kinase 2 XR_001783745.1 45% (many diffs)
39 mouse 70762 Dclk2 doublecortin-like kinase 2 XR_867229.1 28.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
102
ORF length:
36
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cagcacccag gagtatcgag ctggagcact ttgaggaacg ggacaaaagg
121ccgcggccgg ggtcgcggag aggggccccc agctcctccg ggggcagcag cagctcgggc
181cccaagggga acgggctcat ccccagtccg gcgcacagtg cccactgcag cttctaccgc
241acgcggaccc tgcaggccct cagctcggag aagaaggcca agaaggcgcg cttctaccgg
301aacggggacc gctacttcaa gggcctggtg tttgccatct ccagcgaccg cttccggtcc
361ttcgatgcgc tcctcataga gctcacccgc tccctgtcgg acaacgtgaa cctgccccag
421ggtgtccgca ctatctacac catcgacggc agccggaagg tcaccagcct ggacgagctg
481ctggaaggtg agagttacgt gtgtgcatcc aatgaaccat ttcgtaaagt cgattacacc
541aaaaatatta atccaaactg gtctgtgaac atcaagggtg ggacatcccg agcgctggct
601gctgcctcct ctgtgaaaag tgaagtaaaa gaaagtaaag atttcatcaa acccaagtta
661gtgactgtga ttcgaagtgg agtgaagcct agaaaagccg tgcggatcct tctgaataaa
721aagactgctc attcctttga acaagtctta acagatatca ccgaagccat taaactagac
781tcaggagtcg tcaagaggct ctgcaccctg gatggaaagc aggttacttg tctgcaagac
841ttttttggtg atgacgatgt ttttattgca tgtggaccag aaaaatttcg ttatgcccaa
901gatgactttg tcctggatca tagtgaatgt cgtgtcctga agtcatctta ttctcgatcc
961tcagctgtta agtattctgg atccaaaagc cctgggccct ctcgacgcag caaatcacca
1021gcttcagtta atggaactcc cagcagccaa ctttctactc ctaaatctac gaaatcctcc
1081agttcctctc caactagtcc aggaagtttc agaggattaa agatttctgc tcatggcaga
1141tcttcttcca atgtaaacgg tggacctgag cttgaccgtt gcataagtcc tgaaggtgtg
1201aatggaaaca gatgctctga atcatcaact cttcttgaga aatacaaaat tggaaaggtc
1261attggtgatg gcaattttgc agtagtcaaa gagtgtatag acaggtccac tggaaaggag
1321tttgccctaa agattataga caaagccaaa tgttgtggaa aggaacacct gattgagaat
1381gaagtgtcaa tactgcgccg agtgaaacat cccaatatca ttatgctggt cgaggagatg
1441gaaacagcaa ctgagctctt tctggtgatg gaattggtca aaggtggaga tctctttgat
1501gcaattactt cgtcgaccaa gtacactgag agagatggca gtgccatggt gtacaactta
1561gccaatgccc tcaggtatct ccatggcctc agcatcgtgc acagagacat caaaccagag
1621aatctcttgg tgtgtgaata tcctgatgga accaagtctt tgaaactggg agactttggg
1681cttgcgactg tggtagaagg ccctttatac acagtctgtg gcacacccac ttatgtggct
1741ccagaaatca ttgctgaaac tggctatggc ctgaaggtgg acatttgggc agctggtgtg
1801atcacataca tacttctctg tggattccca ccattccgaa gtgagaacaa TCTCCAGGAA
1861GATCTCTTCG ACCAGATCTT GGCTGGGAAG CTGGAGTTTC CGGCCCCCTA CTGGGATAAC
1921ATCACGGACT CTGCCAAGGA ATTAATCAGT CAAATGCTTC AGGTAAATGT TGAAGCTCGG
1981TGTACCGCGG GACAAATCCT GAGTCACCCC TGGGTGTCAG ATGATGCCTC CCAGGAGAAT
2041AACATGCAAG CTGAGGTGAC AGGTAAACTA AAACAGCACT TTAATAATGC GCTCCCCAAA
2101CAGAACAGCA CTACCACCGG GGTCTCCGTC ATCATGTTTG ATTTGACAGT TTGCCCAACT
2161TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT
2221TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA
2281TCTTGTGGAA AGGACGAAAC TGCGCTTGTT TCCCTCCGCC AACGCGTTAA GTCgacaatc
2341aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)