Construct: ORF TRCN0000475957
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002529.1_s317c1
- Derived from:
- ccsbBroadEn_13919
- DNA Barcode:
- AAGTCTTGGATCATGTTCTGTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PGM5 (5239)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475957
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5239 | PGM5 | phosphoglucomutase 5 | XM_011518782.3 | 74.7% | 73.6% | (many diffs) |
| 2 | human | 5239 | PGM5 | phosphoglucomutase 5 | XM_024447573.1 | 74.7% | 73.6% | (many diffs) |
| 3 | human | 5239 | PGM5 | phosphoglucomutase 5 | XM_011518783.3 | 74.5% | 73.3% | (many diffs) |
| 4 | human | 5239 | PGM5 | phosphoglucomutase 5 | NM_021965.4 | 57.2% | 56.9% | (many diffs) |
| 5 | human | 595135 | PGM5P2 | phosphoglucomutase 5 pseudo... | NR_002836.2 | 47.6% | (many diffs) | |
| 6 | mouse | 226041 | Pgm5 | phosphoglucomutase 5 | NM_175013.2 | 52.3% | 55.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggtggtcagc gacagcaggt actttagcag gacggccatc gagatcgtgg 121 tgcagatggc cgcggccaac gggattggac gactgattat tggacagaat ggcatcttgt 181 cgacacctgc ggtctcctgc attatcagga agatcaaggc agctggtgga atcattctaa 241 cagccagcca ctgccctgga ggaccagggg gagagtttgg agtgaagttt aatgttgcca 301 atggaggtcc tgcacccgat gttgtctcag acaaaatcta ccaaatcagc aaaacgattg 361 aggaatatgc tatatgtcct gatctccgaa tcgacctatc tcgactagga agacaagaat 421 ttgacctaga aaacaaattc aaaccattca gagtggagat agtggaccca gtggatatct 481 atcttaacct ccttcggacc atctttgact ttcatgccat caagggtttg ctgactggac 541 ccagccaact gaagattcgc attgacgcaa tgcacggagt tatgggacct tatgtgagaa 601 aagttctgtg tgatgagctg ggggccccag ccaattctgc aataaactgt gttcccctgg 661 aagactttgg agggcagcac cctgacccaa acctgacata tgcaacgact cttctggaag 721 caatgaaagg aggagaatat ggatttGGAG CTGCATTTGA TGCTGATGGG GACCGTTATA 781 TGATCCTAGG CCAAAATGGC TTCTTTGTGA GCCCTTCTGA CTCCCTGGCC ATCATTGCTG 841 CCAACCTCTC TTGCATTCCA TATTTCCGTC AGATGGGGGT CCGCGGGTTT GGGAGGAGTA 901 TGCCAACCAG CATGGCCCTG GACAGAGTGG CCAAATCAAT GAAGGTCCCT GTATATGAGA 961 CCCCAGCTGG ATGGAGATTC TTCTCAAATC TGATGGACTC AGGACGTTGC AATCTGTGTG 1021 GGGAAGAGAG CTTTGGCACT GTTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA AGTCTTGGAT 1201 CATGTTCTGT ATAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att