Construct: ORF TRCN0000476014
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015883.1_s317c1
- Derived from:
- ccsbBroadEn_03827
- DNA Barcode:
- TTGCCGCTCATATGCTACTTAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCTD16 (57528)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476014
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57528 | KCTD16 | potassium channel tetrameri... | NM_001370486.1 | 100% | 100% | |
| 2 | human | 57528 | KCTD16 | potassium channel tetrameri... | NM_001370487.1 | 100% | 100% | |
| 3 | human | 57528 | KCTD16 | potassium channel tetrameri... | NM_020768.4 | 100% | 100% | |
| 4 | human | 57528 | KCTD16 | potassium channel tetrameri... | XM_005268493.2 | 100% | 100% | |
| 5 | human | 57528 | KCTD16 | potassium channel tetrameri... | XM_011537671.2 | 100% | 100% | |
| 6 | human | 57528 | KCTD16 | potassium channel tetrameri... | XM_011537672.2 | 67.3% | 65.2% | (many diffs) |
| 7 | human | 57528 | KCTD16 | potassium channel tetrameri... | XM_011537673.2 | 66.1% | 64.7% | (many diffs) |
| 8 | mouse | 383348 | Kctd16 | potassium channel tetrameri... | NM_026135.1 | 89.4% | 96.2% | (many diffs) |
| 9 | mouse | 383348 | Kctd16 | potassium channel tetrameri... | XM_017317936.1 | 89.4% | 96.2% | (many diffs) |
| 10 | mouse | 383348 | Kctd16 | potassium channel tetrameri... | XM_017317937.1 | 89.4% | 96.2% | (many diffs) |
| 11 | mouse | 383348 | Kctd16 | potassium channel tetrameri... | XM_017317938.1 | 89.4% | 96.2% | (many diffs) |
| 12 | mouse | 383348 | Kctd16 | potassium channel tetrameri... | XM_017317939.1 | 89.4% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1353
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctctgagt ggaaactgta gtcgttatta tcctcgagaa caagggtccg 121 cagttcccaa ctccttccct gaggtggtag agctgaatgt cgggggtcaa gtttatttta 181 ctcgccattc cacattgata agcatccctc attccctcct gtggaaaatg ttttccccaa 241 agagagacac ggctaatgat ctagccaagg actccaaggg aaggtttttc attgacagag 301 atggattctt gttccgttat attctggact atctcaggga caggcaggtg gtcctgcctg 361 atcactttcc agaaaaagga agactgaaaa gggaagctga atacttccag ctcccagact 421 tggtcaaact cctgaccccc gatgaaatca agcaaagccc agatgaattc tgccacagtg 481 actttgaaga tgcctcccaa ggaagcgaca caagaatctg ccccccttcc tccctgctcc 541 ctgccgaccg caagtggggt ttcattactg tgggttacag aggatcctgc accttgggca 601 gagagggaca ggcagatgcc aagtttcgga gagttccccg gattttggtt tgtggaagga 661 tttccttggc aaaagaagtc tttggagaaa ctttgaatga aagcagagac cctgatcgag 721 ccccagaaag atacacctcc agattttatc tcaaattcaa gcacctggaa agggcttttg 781 atatgttgtc agagtgtgga ttccacatgg tggcctgtaa ctcatcggtg acagcatctt 841 tcatcaacca atatacagat gacaagatct ggtcaagcta cactgaatat gtcttctacc 901 gtgagccttc cagatggtca ccctcacact gcgattgctg ctgcaagaat ggcaaaggtg 961 acaaagaagg ggagagcggc acgtcttgca atgacctCTC CACATCTAGC TGCGACAGCC 1021 AGTCTGAGGC CAGCTCTCCC CAGGAGACGG TCATCTGTGG TCCCGTGACA CGCCAGACCA 1081 ACATCCAGAC TCTGGACCGT CCCATCAAGA AGGGCCCTGT CCAGCTGATC CAACAGTCAG 1141 AGATGCGGCG GAAAAGCGAC TTACTCCGGA CTCTGACTTC AGGCTCCAGG GAATCGAACA 1201 TGAGCAGCAA AAAAAAAGCT GTTAAAGAAA AGCTCTCAAT TGAGGAGGAG CTGGAGAAAT 1261 GTATCCAGGA TTTCCTAAAA ATCAAAATTC CAGATCGGTT TCCTGAGAGA AAACATCCTT 1321 GGCAATCTGA ACTTTTAAGG AAGTATCATC TATTGCCAAC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1501 GCCGCTCATA TGCTACTTAA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt