Construct: ORF TRCN0000476017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010274.1_s317c1
- Derived from:
- ccsbBroadEn_06506
- DNA Barcode:
- TTGGATAGGGACAAACGGTCAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT13 (3860)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476017
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3860 | KRT13 | keratin 13 | NM_153490.3 | 99.7% | 99.5% | 114C>T;560C>T;892A>G |
| 2 | human | 3860 | KRT13 | keratin 13 | NM_002274.4 | 91.4% | 90.3% | (many diffs) |
| 3 | mouse | 16663 | Krt13 | keratin 13 | NM_010662.2 | 82.7% | 83.6% | (many diffs) |
| 4 | mouse | 16663 | Krt13 | keratin 13 | NM_001313949.1 | 41.6% | 42.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1443
- ORF length:
- 1374
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcctccgc ctgcagagct cctctgccag ctatggaggt ggtttcgggg 121 gtggctcttg ccagctggga ggaggccgtg gtgtctctac ctgttcaact cggtttgtgt 181 ctgggggatc agctgggggc tatggaggcg gcgtgagctg tggttttggt ggaggggctg 241 gtagtggctt tggaggtggc tatggaggtg gccttggagg tggctatgga ggtggccttg 301 gaggtggctt tggtgggggt tttgctggtg gctttgttga ctttggtgct tgtgatggcg 361 gcctcctcac tggcaatgag aagatcacca tgcagaacct caacgaccgc ctggcttcct 421 acctggagaa ggtgcgcgcc ctggaggagg ccaacgctga cctggaggtg aagatccgtg 481 actggcacct gaagcagagc ccagctagcc ctgagcggga ctacagcccc tactacaaga 541 ccattgaaga gctccgggac aagatcctga ccgccaccat tgaaaacaac cgggtcatcc 601 tggagattga caatgccagg ctggctgtgg acgacttcag gctcaagtat gagaatgagc 661 tggccctgcg ccagagcgtg gaggccgaca tcaacggcct gcgccgggtg ctggatgagc 721 tcactctgtc taagactgac ctggagatgc agatcgagag cctgaatgaa gagctagcct 781 acatgaagaa gaaccatgaa gaggagatga aggaatttag caaccaggtg gtcggccagg 841 tcaacgtgga gatggatgcc accccaggca ttgacctgac ccgcgtgctg gcagagatga 901 gggagcagta cgaggccatg gcagagagga accgccggga tgctgaggaa tggttccacg 961 ccaagagtgc agagctgaac aaggaggtgt ctaccaacac tgccatgatt cagaccagca 1021 agacagagat cacggagctc aggcgcacgc TCCAAGGCCT GGAGATTGAG CTGCAGTCCC 1081 AGCTGAGCAT GAAAGCGGGG CTGGAGAACA CGGTGGCAGA GACGGAGTGC CGCTATGCCC 1141 TGCAGCTGCA GCAGATCCAG GGACTCATCA GCAGCATCGA GGCCCAGCTG AGCGAGCTCC 1201 GCAGTGAGAT GGAGTGCCAG AACCAAGAGT ACAAGATGCT GCTGGACATC AAGACACGTC 1261 TGGAGCAGGA GATCGCCACC TACCGCAGCC TGCTCGAGGG CCAGGACGCC AAGATGATTG 1321 GTTTCCCTTC CTCAGCAGGA AGCGTCAGCC CCCGTAGCAC CTCTGTTACC ACGACTTCTA 1381 GTGCCTCTGT TACCACCACC TCTAATGCCT CTGGTCGCCG CACTTCTGAT GTCCGTAGGC 1441 CTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1501 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1561 GGCTTTATAT ATCTTGTGGA AAGGACGATT GGATAGGGAC AAACGGTCAA CGACGCGTTA 1621 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt