Construct: ORF TRCN0000476032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014054.1_s317c1
- Derived from:
- BRDN0000391374
- DNA Barcode:
- CAGCTATCTCGCAGTATGCAGTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GRIN2B (2904)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476032
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2904 | GRIN2B | glutamate ionotropic recept... | NM_000834.4 | 99.7% | (many diffs) | |
| 2 | human | 2904 | GRIN2B | glutamate ionotropic recept... | XM_011520628.2 | 99.7% | (many diffs) | |
| 3 | human | 2904 | GRIN2B | glutamate ionotropic recept... | XM_011520629.2 | 99.7% | (many diffs) | |
| 4 | human | 2904 | GRIN2B | glutamate ionotropic recept... | XM_017019219.2 | 99.7% | (many diffs) | |
| 5 | human | 2904 | GRIN2B | glutamate ionotropic recept... | XM_005253351.3 | 50.1% | 0_1ins2214;1252_1254delAAGinsCCA;1320C>T | |
| 6 | mouse | 14812 | Grin2b | glutamate receptor, ionotro... | NM_008171.3 | 91.2% | (many diffs) | |
| 7 | mouse | 14812 | Grin2b | glutamate receptor, ionotro... | XM_006505573.3 | 91.2% | (many diffs) | |
| 8 | mouse | 14812 | Grin2b | glutamate receptor, ionotro... | XM_006505574.3 | 91.2% | (many diffs) | |
| 9 | mouse | 14812 | Grin2b | glutamate receptor, ionotro... | XM_006505575.3 | 91.2% | (many diffs) | |
| 10 | mouse | 14812 | Grin2b | glutamate receptor, ionotro... | XM_011241220.2 | 91.2% | (many diffs) | |
| 11 | mouse | 14812 | Grin2b | glutamate receptor, ionotro... | XM_011241221.2 | 91.2% | (many diffs) | |
| 12 | mouse | 14812 | Grin2b | glutamate receptor, ionotro... | XM_017321396.1 | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 87
- ORF length:
- 18
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagcccaga gcggagtgat gacttcccaa gttctggttg gtgttggccg 121 tcctggccgt gtcaggcagc agagctcgtt ctcagaagag cccccccagc attggcattg 181 ctgtcatcct cgtgggcact tccgacgagg tggccatcaa ggatgcccac gagaaagatg 241 atttccacca tctctccgtg gtaccccggg tggaactggt agccatgaat gagaccgacc 301 caaagagcat catcacccgc atctgtgatc tcatgtctga ccggaagatc cagggggtgg 361 tgtttgctga tgacacagac caggaagcca tcgcccagat cctcgatttc atttcagcac 421 agactctcac cccgatcctg ggcatccacg ggggctcctc tatgataatg gcagataagg 481 atgaatcctc catgttcttc cagtttggcc catcaattga acagcaagct tccgtaatgc 541 tcaacatcat ggaagaatat gactggtaca tcttttctat cgtcaccacc tatttccctg 601 gctaccagga ctttgtaaac aagatccgca gcaccattga gaatagcttt gtgggctggg 661 agctagagga ggtcctccta ctggacatgt ccctggacga tggagattct aagatccaga 721 atcagctcaa gaaacttcaa agccccatca ttcttcttta ctgtaccaag gaagaagcca 781 cctacatctt tgaagtggcc aactcagtag ggctgactgg ctatggctac acgtggatcg 841 tgcccagtct ggtggcaggg gatacagaca cagtgcctgc ggagttcccc actgggctca 901 tctctgtatc atatgatgaa tgggactatg gcctccccgc cagagtgaga gatggaattg 961 ccataatcac cactgctgct tctgacatgc tgtctgagca cagcttcatc cctgagccca 1021 aaagcagttg ttacaacacc cacgagaaga gaatctacca gtccaatatg ctaaataggt 1081 atctgatcaa tgtcactttt gaggggagga atttgtcctt cagtgaagat ggctaccaga 1141 tgcacccgaa actggtgata attcttctga acaaggagag gaagtgggaa agggtgggga 1201 agtggaaaga caagtccctg cagatgaagt actatgtgtg gccccgaatg tgtccagaga 1261 ctgaagagca ggaggatgac catctgagca ttgtgaccct ggaggaggca ccatttgtca 1321 ttgtggaaag tgtggaccct ctgagtggaa cctgcatgag gaacacagtc ccctgccaaa 1381 aacgcatagt cactgagaat aaaacagacg aggagccggg ttacatcaaa aaatgctgca 1441 aggggttctg tattgacatc cttaagaaaa tttctaaatc tgtgaagttc acctatgacc 1501 tttacctggt taccaatggc aagcatggga agaaaatcaa tggaacctgg aatggtatga 1561 ttggagaggt ggtcatgaag agggcctaca tggcagtggg ctcactcacc atcaatgagg 1621 aacgatcgga ggtggtcgac ttctctgtgc ccttcataga gacaggcatc agtgtcatgg 1681 tgtcacgcag caatgggact gtctcacctt ctgccttctt agagccattc agtgctgacg 1741 tatgggtgat gatgtttgtg atgctgctca tcgtctcagc cgtggctgtc tttgtctttg 1801 agtacttcag ccctgtgggt tataacaggt gcctcgctga tggcagagag cctggtggac 1861 cctctttcac catcggcaaa gctatttggt tgctctgggg tctggtgttt aacaactccg 1921 tacctgtgca gaacccaaag gggaccacct ccaagatcat ggtgtcagtg tgggccttct 1981 ttgctgtcat cttcctggcc agctacactg ccaacttagc tgccttcatg atccaagagg 2041 aatatgtgga ccaggtttct ggcctgagcg acaaaaagtt ccagagacct aatgacttct 2101 cacccccttt ccgctttggg accgtgccca acggcagcac agagagaaat attcgcaata 2161 actatgcaga aatgcatgcc tacatgggaa agttcaacca gaggggtgta gatgatgcat 2221 tgctctccct gaaaacaggg aaactggatg ccttcatcta tgatgcagca gtgctgaact 2281 atatggcagg cagagatgaa ggctgcaagc tggtgaccat tggcagtggg aaggtctttg 2341 cttccactgg ctatggcatt gccatccaaa aagattctgg gtggaagcgc caggtggacc 2401 ttgctatcct gcagctcttt ggagatgggg agatggaaga actggaagct ctctggctca 2461 ctggcatttg tcacaatgag aagaatgagg tcatgagcag ccagctggac attgacaaca 2521 tggcaggggt cttctacatg ttgggggcgg ccatggctct cagcctcatc accttcatct 2581 gcgaacacct tttctattgg cagttccgac attgctttat gggtgtctgt tctggcaagc 2641 ctggcatggt cttctccatc agcagaggta tctacagctg catccatggg gtggcgatcg 2701 aggagcgcca gtctgtaatg aactccccca ccgcaaccat gaacaacaca cactccaaca 2761 tcctgcgcct gctgcgcacg gccaagaaca tggctaacct gtctggtgtg aatggctcac 2821 cgcagagcgc cctggacttc atccgacggg agtcatccgt ctatgacatc tcagagcacc 2881 gccgcagctt cacgcattct gactgcaaat cctacaacaa cccgccctgt gaggagaacc 2941 tcttcagtga ctacatcagt gaggtagaga gaacgttcgg gaacctgcag ctgaaggaca 3001 gcaacgtgta ccaagatcac taccaccatc accaccggcc ccatagtatt ggcagtgcca 3061 gctccatcga tgggctctac gactgtgaca acccaccctt caccacccag tccaggtcca 3121 tcagcaagaa gcccctggac atcggcctcc cctcctccaa gcacagccag ctcagtgacc 3181 tgtacggcaa attctccttc aagagcgacc gctacagtgg ccacgacgac ttgatccgct 3241 ccgatgtctc tgacatctca acccacaccg tcacctatgg gaacatcgag ggcaatgccg 3301 ccaagaggcg taagcagcaa tataaggaca gcctgaagaa gcggcctgcc tcggccaagt 3361 cccgcaggga gtttgacgag atcgagctgg cctaccgtcg ccgaccgccc cgctcccctg 3421 accacaagcg ctacttcagg gacaaggaag ggctacggga cttctacctg gaccagttcc 3481 gaacaaagga gaactcaccc cactgggagc acgtagacct gaccgacatc tacccagagc 3541 ggagtgatga ctttaagcgc gactccgtca gcggaggagg gccctgtacc aacaggtctc 3601 atatcaagca cgggacgggc gacaaacacg gcgtggtcag cggggtacct gcaccttggg 3661 agaagaacct gaccaacgtg gagtgggagg accggtccgg gggcaacttc tgccgcagct 3721 gtccctccaa gctgcacaac tactccacga cggtgacggg tcagaactcg ggcaggcagg 3781 cgtgcatccg gtgtgaggct tgcaagaaag caggcaacct gtatgacatc agtgaggaca 3841 actccctgca ggaactggac cagccggctg ccccagtggc ggtgacgtca aacgcctcca 3901 ccactaagta ccctcagagc ccgactaatt ccaaggccca gaagaagaac cggaacaaac 3961 tgcgccggca gcactcctac gacaccttcg tggacctgca gaaggaagaa gccgccctgg 4021 ccccgcgcag cgtaagcctg aaagacaagg gccgattcat ggatgggagc ccctacgccc 4081 acatgtttga gatgtcagct ggcgagagca cctttgccaa caacaagtcc tcagtgccca 4141 ctgccggaca TCACCACCAC AACAACCCCG GCGGCGGGTA CATGCTCAGC AAGTCGCTCT 4201 ACCCTGACCG GGTCACGCAA AACCCTTTCA TCCCCACTTT TGGGGACGAC CAGTGCTTGC 4261 TCCATGGCAG CAAATCCTAC TTCTTCAGGC AGCCCACGGT GGCGGGGGCG TCGAAAGCCA 4321 GGCCGGACTT CCGGGCCCTT GTCACCAACA AGCCGGTGGT CTCGGCCCTT CATGGGGCCG 4381 TGCCAGCCCG TTTCCAGAAG GACATCTGTA TAGGGAACCA GTCCAACCCC TGTGTGCCTA 4441 ACAACAAAAA CCCCAGGGCT TTCAATGGCT CCAGCAATGG GCATGTTTAT GAGAAACTTT 4501 CTAGTATTGA GTCTGATGTC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 4561 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 4621 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAGC TATCTCGCAG 4681 TATGCAGTTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt