Construct: ORF TRCN0000476062
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002351.1_s317c1
- Derived from:
- ccsbBroadEn_06096
- DNA Barcode:
- AATTCATTAACCGGCTCGGCACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSDME (1687)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476062
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1687 | GSDME | gasdermin E | NM_001127453.2 | 99.7% | 99.3% | (many diffs) |
| 2 | human | 1687 | GSDME | gasdermin E | NM_004403.3 | 99.7% | 99.3% | (many diffs) |
| 3 | human | 1687 | GSDME | gasdermin E | XM_024446670.1 | 99.7% | 99.3% | (many diffs) |
| 4 | human | 1687 | GSDME | gasdermin E | NM_001127454.2 | 66.7% | 66.3% | (many diffs) |
| 5 | human | 1687 | GSDME | gasdermin E | XM_017011802.1 | 66.7% | 66.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1557
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttgccaaa gcaaccagga attttcttag agaagttgat gctgatggtg 121 acctgattgc agtatcaaat ctgaatgact ctgataagtt acagcttcta agtctggtga 181 caaaaaagaa gagattctgg tgctggcaga gacccaagta ccagttttta tccctcaccc 241 ttggcgatgt actcatagaa gaccaatttc cgagtccagt ggtcgtggag tcggactttg 301 tgaaatacga gggcaagttt gcaaaccacg tgagtggaac cctggagact gcactgggga 361 aggtcaagct gaacctgggg ggcagcagcc gcgtagagag ccagtcttca tttggaaccc 421 tgaggaagca ggaggtggat ttgcagcagc tcatcagaga ctctgccgag agaacaataa 481 atctgagaaa ccctgtgctc cagcaggtgc tggagggaag gaatgaggtc ctgtgcgttt 541 tgacacagaa gatcacgacg atgcagaagt gtgtgatctc tgagcacatg caggtcgagg 601 agaagtgtgg tggcatcgtg ggcatccaga ccaagacggt gcaggtgtca gcgacggagg 661 atgggaatgt caccaaggac tccaacgtgg tgctggagat cccagctgcc accaccattg 721 cctacggtgt cattgagtta tacgtgaaac tggacggcct gttcgagttc tgccttctcc 781 gagggaagca aggtggcttc gagaacaaga agagaattga ctctgtctac ctggaccacc 841 tggtctttcg agagtttgca ttcatagaca tgccagatgc tgcgcatggg atatcttccc 901 aggatggacc attaagtgtt ttaaagcaag cgaccctgct cctggagagg aatttccatc 961 catttgcgga gctgcctgag ccacaacaga cagctttgag tgacatcttc caggcggtcg 1021 tatttgatga tgaactactc atggtcctgg aaccagtgtg cgatgacctg gtcagcggcc 1081 tctcgcccac agtggcggtg ctgggggagc tgaagccccg gcagcagcag gaccttgtgg 1141 ccttcctgca gctggtgggg tgcagcttac agggtgggtg tccgggcccc GAGGATGCAG 1201 GCAGCAAGCA GCTGTTTATG ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG 1261 ATAGCGCAGC AGCTCTGCTG GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC 1321 ACTTGCTTCG TGCTCTGTCT GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC 1381 CCCTGAAAGA TACAGAAAGG TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA 1441 GTCTGGAGAG ACTGAAGTCA TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC 1501 CACTGCTTCT TTGTATAACC CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATTGC 1561 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1621 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1681 ATATATCTTG TGGAAAGGAC GAAATTCATT AACCGGCTCG GCACCAACGC GTTAAGTCga 1741 caatcaacct ctggattaca aaatttgtga aagatt