Construct: ORF TRCN0000476079

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001306.4_s317c1
Derived from:
ccsbBroadEn_00944
DNA Barcode:
ACTATAGCACACTGAAGGTTTGAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LIMK2 (3985)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476079
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3985 LIMK2 LIM domain kinase 2 NM_001031801.2 100% 100%
2 human 3985 LIMK2 LIM domain kinase 2 NM_016733.3 87.6% 78.3% (many diffs)
3 human 3985 LIMK2 LIM domain kinase 2 NM_005569.4 83.9% 74.3% (many diffs)
4 human 26472 PPP1R14B protein phosphatase 1 regul... NM_138689.3 18.9% 15.5% (many diffs)
5 mouse 16886 Limk2 LIM motif-containing protei... NM_173053.1 78.3% 74.6% (many diffs)
6 mouse 16886 Limk2 LIM motif-containing protei... XM_006514550.3 77.6% 74% (many diffs)
7 mouse 16886 Limk2 LIM motif-containing protei... NM_010718.4 75.4% 71.3% (many diffs)
8 mouse 16886 Limk2 LIM motif-containing protei... XM_006514548.2 74.8% 70.8% (many diffs)
9 mouse 16886 Limk2 LIM motif-containing protei... XM_011243666.2 70.1% 66.8% (many diffs)
10 mouse 16886 Limk2 LIM motif-containing protei... XM_006514547.1 69.6% 66.2% (many diffs)
11 mouse 16886 Limk2 LIM motif-containing protei... NM_001034030.2 56.8% 53.1% (many diffs)
12 mouse 16886 Limk2 LIM motif-containing protei... XM_006514552.3 56.3% 52.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2124
ORF length:
2058
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gagttacttg tcagtcccgg cttacttcac ctccagagac ctgtttcggt
121gttcagaatg ccaggattcc ctcaccaact ggtactatga gaaggatggg aagctctact
181gccccaagga ctactggggg aagtttgggg agttctgtca tgggtgctcc ctgctgatga
241cagggccttt tatggtggct ggggagttca agtaccaccc agagtgcttt gcctgtatga
301gctgcaaggt gatcattgag gatggggatg catatgcact ggtgcagcat gccaccctct
361actgtgggaa gtgccacaat gaggtggtgc tggcacccat gtttgagaga ctctccacag
421agtctgttca ggagcagctg ccctactctg tcacgctcat ctccatgccg gccaccactg
481aaggcaggcg gggcttctcc gtgtccgtgg agagtgcctg ctccaactac gccaccactg
541tgcaagtgaa agaggtcaac cggatgcaca tcagtcccaa caatcgaaac gccatccacc
601ctggggaccg catcctggag atcaatggga cccccgtccg cacacttcga gtggaggagg
661tggaggatgc aattagccag acgagccaga cacttcagct gttgattgaa catgaccccg
721tctcccaacg cctggaccag ctgcggctgg aggcccggct cgctcctcac atgcagaatg
781ccggacaccc ccacgccctc agcaccctgg acaccaagga gaatctggag gggacactga
841ggagacgttc cctaaggcgc agtaacagta tctccaagtc ccctggcccc agctccccaa
901aggagcccct gctgttcagc cgtgacatca gccgctcaga atcccttcgt tgttccagca
961gctattcaca gcagatcttc cggccctgtg acctaatcca tggggaggtc ctggggaagg
1021gcttctttgg gcaggctatc aaggtgacac acaaagccac gggcaaagtg atggtcatga
1081aagagttaat tcgatgtgat gaggagaccc agaaaacttt tctgactgag gtgaaagtga
1141tgcgcagcct ggaccacccc aatgtgctca agttcattgg tgtgctgtac aaggataaga
1201agctgaacct cctgacagag tacattgagg ggggcacact gaaggacttt ctgcgcagta
1261tggatccgtt cccctggcag cagaaggtca ggtttgccaa aggaatcgcc tccggaatgg
1321cctatttgca ctctatgtgc atcatccacc gggatctgaa ctcgcacaac tgcctcatca
1381agttggacaa gactgtggtg gtggcagact ttgggctgtc acggctcata gtggaagaga
1441ggaaaagggc ccccatggag aaggccacca ccaagaaacg caccttgcgc aagaacgacc
1501gcaagaagcg ctacacggtg gtgggaaacc cctactggat ggcccctgag atgctgaacg
1561gaaagagcta tgatgagacg gtggatatct tctcctttgg gatcgttctc tgtgagatca
1621ttgggcaggt gtatgcagat cctgactgcc ttccccgaac actggacttt ggcctcaacg
1681tgaagctttt ctgggagaag tttgttccca cagattgtcc cccggccttc ttcccgctgg
1741ccgccatctg ctgcagactg gagccTGAGA GCAGAGCCCC CCCCGGGGCC GCAGGAGAGG
1801GCCCGGGCTG CGCGGATGAT GAGGGCCCAG TGAGGCGCCA AGGGAAGGTC ACCATCAAGT
1861ATGACCCCAA GGAGCTACGG AAGCACCTCA ACCTAGAGGA GTGGATCCTG GAGCAGCTCA
1921CGCGCCTCTA CGACTGCCAG GAAGAGGAGA TCTCAGAACT AGAGATTGAC GTGGATGAGC
1981TCCTGGACAT GGAGAGTGAC GATGCCTGGG CTTCCAGGGT CAAGGAGCTG CTGGTTGACT
2041GTTACAAACC CACAGAGGCC TTCATCTCTG GCCTGCTGGA CAAGATCCGG GCCATGCAGA
2101AGCTGAGCAC ACCCCAGAAG AAGAGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
2161AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
2221TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CTATAGCACA
2281CTGAAGGTTT GAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2341att

Download FASTA (ORF) (Full)