Construct: ORF TRCN0000476123

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006467.1_s317c1
Derived from:
ccsbBroadEn_06974
DNA Barcode:
ATTCCGCTAATGAGCCTCCCGTCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC22A5 (6584)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476123
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6584 SLC22A5 solute carrier family 22 me... NM_003060.4 99.9% 99.8% 341T>C
2 human 6584 SLC22A5 solute carrier family 22 me... NM_001308122.1 95.8% 95.6% 341T>C;392_463del
3 human 6584 SLC22A5 solute carrier family 22 me... XM_017009778.2 68.4% 68.4% 0_1ins528
4 human 6584 SLC22A5 solute carrier family 22 me... XM_011543590.2 61.7% 61.5% 0_1ins631;21_33del
5 human 6584 SLC22A5 solute carrier family 22 me... XR_948291.2 49.5% (many diffs)
6 human 6584 SLC22A5 solute carrier family 22 me... XR_427718.2 49.4% (many diffs)
7 human 6584 SLC22A5 solute carrier family 22 me... XR_001742215.1 47.1% (many diffs)
8 human 6584 SLC22A5 solute carrier family 22 me... XR_001742216.1 46.9% (many diffs)
9 human 6584 SLC22A5 solute carrier family 22 me... XR_948290.2 43.3% (many diffs)
10 mouse 20520 Slc22a5 solute carrier family 22 (o... NM_011396.3 86.7% 85.2% (many diffs)
11 mouse 20520 Slc22a5 solute carrier family 22 (o... XM_006532650.2 80.2% 72.3% (many diffs)
12 mouse 20520 Slc22a5 solute carrier family 22 (o... XM_006532651.3 59.1% 58.8% (many diffs)
13 mouse 20520 Slc22a5 solute carrier family 22 (o... XM_006532652.1 48.6% 48.1% (many diffs)
14 mouse 20520 Slc22a5 solute carrier family 22 (o... XM_017314397.1 48.6% 48.1% (many diffs)
15 mouse 20520 Slc22a5 solute carrier family 22 (o... XR_001779934.1 45.1% (many diffs)
16 mouse 20520 Slc22a5 solute carrier family 22 (o... XR_001779933.1 45% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1737
ORF length:
1671
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcg ggactacgac gaggtgaccg ccttcctggg cgagtggggg cccttccagc
121gcctcatctt cttcctgctc agcgccagca tcatccccaa tggcttcacc ggcctgtcct
181ccgtgttcct gatagcgacc ccggagcacc gctgccgggt gccggacgcc gcgaacctga
241gcagcgcctg gcgcaaccac actgtcccac tgcggctgcg ggacggccgc gaggtgcccc
301acagctgccg ccgctaccgg ctcgccacca tcgccaactt ctcggcgctt gggctggagc
361cggggcgcga cgtggacctg gggcagctgg agcaggagag ctgtccggat ggctgggagt
421tcagtcagga cgtctacctg tccaccattg tgaccgagtg gaacctggtg tgtgaggacg
481actggaaggc cccactcaca atctccttgt tcttcgtggg tgtgctgttg ggctccttca
541tttcagggca gctgtcagac aggtttggcc ggaagaatgt gctgttcgtg accatgggca
601tgcagacagg cttcagcttc ctgcagatct tctcgaagaa ttttgagatg tttgtcgtgc
661tgtttgtcct tgtaggcatg ggccagatct ccaactatgt ggcagcattt gtcctgggga
721cagaaattct tggcaagtca gttcgtataa tattctctac gttaggagtg tgcatatttt
781atgcatttgg ctacatggtg ctgccactgt ttgcttactt catccgagac tggcggatgc
841tgctggtggc gctgacgatg ccgggggtgc tatgcgtggc actctggtgg ttcatccctg
901agtccccccg atggctcatc tctcagggac gatttgaaga ggcagaggtg atcatccgca
961aggctgccaa agccaatggg attgttgtgc cttccactat ctttgacccg agtgagttac
1021aagacctaag ttccaagaag cagcagtccc acaacattct ggatctgctt cgaacctgga
1081atatccggat ggtcaccatc atgtccataa tgctgtggat gaccatatca gtgggctatt
1141ttgggctttc gcttgatact cctaacttgc atggggacat ctttgtgaac tgcttccttt
1201cagcgatggt tgaagtccca gcatatgtgt tggcctggct gctgctgcaa tatttgcccc
1261ggcgctattc catggccact gccctcttcc tgggtggcag tgtccttctc ttcatgcagc
1321tggtaccccc agacttgtat tatttggcta cagtcctggt gatggtgggc aagtttGGAG
1381TCACGGCTGC CTTTTCCATG GTCTACGTGT ACACAGCCGA GCTGTATCCC ACAGTGGTGA
1441GAAACATGGG TGTGGGAGTC AGCTCCACAG CATCCCGCCT GGGCAGCATC CTGTCTCCCT
1501ACTTCGTTTA CCTTGGTGCC TACGACCGCT TCCTGCCCTA CATTCTCATG GGAAGTCTGA
1561CCATCCTGAC AGCCATCCTC ACCTTGTTTC TCCCAGAGAG CTTCGGTACC CCACTCCCAG
1621ACACCATTGA CCAGATGCTA AGAGTCAAAG GAATGAAACA CAGAAAAACT CCAAGTCACA
1681CAAGGATGTT AAAAGATGGT CAAGAAAGGC CCACAATCCT TAAAAGCACA GCCTTCTACC
1741CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1801TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1861ATATATCTTG TGGAAAGGAC GAATTCCGCT AATGAGCCTC CCGTCAACGC GTTAAGTCga
1921caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)