Construct: ORF TRCN0000476123
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006467.1_s317c1
- Derived from:
- ccsbBroadEn_06974
- DNA Barcode:
- ATTCCGCTAATGAGCCTCCCGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A5 (6584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476123
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6584 | SLC22A5 | solute carrier family 22 me... | NM_003060.4 | 99.9% | 99.8% | 341T>C |
| 2 | human | 6584 | SLC22A5 | solute carrier family 22 me... | NM_001308122.1 | 95.8% | 95.6% | 341T>C;392_463del |
| 3 | human | 6584 | SLC22A5 | solute carrier family 22 me... | XM_017009778.2 | 68.4% | 68.4% | 0_1ins528 |
| 4 | human | 6584 | SLC22A5 | solute carrier family 22 me... | XM_011543590.2 | 61.7% | 61.5% | 0_1ins631;21_33del |
| 5 | human | 6584 | SLC22A5 | solute carrier family 22 me... | XR_948291.2 | 49.5% | (many diffs) | |
| 6 | human | 6584 | SLC22A5 | solute carrier family 22 me... | XR_427718.2 | 49.4% | (many diffs) | |
| 7 | human | 6584 | SLC22A5 | solute carrier family 22 me... | XR_001742215.1 | 47.1% | (many diffs) | |
| 8 | human | 6584 | SLC22A5 | solute carrier family 22 me... | XR_001742216.1 | 46.9% | (many diffs) | |
| 9 | human | 6584 | SLC22A5 | solute carrier family 22 me... | XR_948290.2 | 43.3% | (many diffs) | |
| 10 | mouse | 20520 | Slc22a5 | solute carrier family 22 (o... | NM_011396.3 | 86.7% | 85.2% | (many diffs) |
| 11 | mouse | 20520 | Slc22a5 | solute carrier family 22 (o... | XM_006532650.2 | 80.2% | 72.3% | (many diffs) |
| 12 | mouse | 20520 | Slc22a5 | solute carrier family 22 (o... | XM_006532651.3 | 59.1% | 58.8% | (many diffs) |
| 13 | mouse | 20520 | Slc22a5 | solute carrier family 22 (o... | XM_006532652.1 | 48.6% | 48.1% | (many diffs) |
| 14 | mouse | 20520 | Slc22a5 | solute carrier family 22 (o... | XM_017314397.1 | 48.6% | 48.1% | (many diffs) |
| 15 | mouse | 20520 | Slc22a5 | solute carrier family 22 (o... | XR_001779934.1 | 45.1% | (many diffs) | |
| 16 | mouse | 20520 | Slc22a5 | solute carrier family 22 (o... | XR_001779933.1 | 45% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1737
- ORF length:
- 1671
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ggactacgac gaggtgaccg ccttcctggg cgagtggggg cccttccagc 121 gcctcatctt cttcctgctc agcgccagca tcatccccaa tggcttcacc ggcctgtcct 181 ccgtgttcct gatagcgacc ccggagcacc gctgccgggt gccggacgcc gcgaacctga 241 gcagcgcctg gcgcaaccac actgtcccac tgcggctgcg ggacggccgc gaggtgcccc 301 acagctgccg ccgctaccgg ctcgccacca tcgccaactt ctcggcgctt gggctggagc 361 cggggcgcga cgtggacctg gggcagctgg agcaggagag ctgtccggat ggctgggagt 421 tcagtcagga cgtctacctg tccaccattg tgaccgagtg gaacctggtg tgtgaggacg 481 actggaaggc cccactcaca atctccttgt tcttcgtggg tgtgctgttg ggctccttca 541 tttcagggca gctgtcagac aggtttggcc ggaagaatgt gctgttcgtg accatgggca 601 tgcagacagg cttcagcttc ctgcagatct tctcgaagaa ttttgagatg tttgtcgtgc 661 tgtttgtcct tgtaggcatg ggccagatct ccaactatgt ggcagcattt gtcctgggga 721 cagaaattct tggcaagtca gttcgtataa tattctctac gttaggagtg tgcatatttt 781 atgcatttgg ctacatggtg ctgccactgt ttgcttactt catccgagac tggcggatgc 841 tgctggtggc gctgacgatg ccgggggtgc tatgcgtggc actctggtgg ttcatccctg 901 agtccccccg atggctcatc tctcagggac gatttgaaga ggcagaggtg atcatccgca 961 aggctgccaa agccaatggg attgttgtgc cttccactat ctttgacccg agtgagttac 1021 aagacctaag ttccaagaag cagcagtccc acaacattct ggatctgctt cgaacctgga 1081 atatccggat ggtcaccatc atgtccataa tgctgtggat gaccatatca gtgggctatt 1141 ttgggctttc gcttgatact cctaacttgc atggggacat ctttgtgaac tgcttccttt 1201 cagcgatggt tgaagtccca gcatatgtgt tggcctggct gctgctgcaa tatttgcccc 1261 ggcgctattc catggccact gccctcttcc tgggtggcag tgtccttctc ttcatgcagc 1321 tggtaccccc agacttgtat tatttggcta cagtcctggt gatggtgggc aagtttGGAG 1381 TCACGGCTGC CTTTTCCATG GTCTACGTGT ACACAGCCGA GCTGTATCCC ACAGTGGTGA 1441 GAAACATGGG TGTGGGAGTC AGCTCCACAG CATCCCGCCT GGGCAGCATC CTGTCTCCCT 1501 ACTTCGTTTA CCTTGGTGCC TACGACCGCT TCCTGCCCTA CATTCTCATG GGAAGTCTGA 1561 CCATCCTGAC AGCCATCCTC ACCTTGTTTC TCCCAGAGAG CTTCGGTACC CCACTCCCAG 1621 ACACCATTGA CCAGATGCTA AGAGTCAAAG GAATGAAACA CAGAAAAACT CCAAGTCACA 1681 CAAGGATGTT AAAAGATGGT CAAGAAAGGC CCACAATCCT TAAAAGCACA GCCTTCTACC 1741 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1801 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1861 ATATATCTTG TGGAAAGGAC GAATTCCGCT AATGAGCCTC CCGTCAACGC GTTAAGTCga 1921 caatcaacct ctggattaca aaatttgtga aagatt