Construct: ORF TRCN0000476132
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001691.1_s317c1
- Derived from:
- ccsbBroadEn_09473
- DNA Barcode:
- GGGACGTCCTCTAATCATGACTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- KLHDC1 (122773)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476132
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122773 | KLHDC1 | kelch domain containing 1 | NM_172193.3 | 99.8% | 22.1% | 271G>T;603A>G |
| 2 | human | 122773 | KLHDC1 | kelch domain containing 1 | XM_011536422.2 | 85.5% | 25.4% | (many diffs) |
| 3 | human | 122773 | KLHDC1 | kelch domain containing 1 | XR_943388.1 | 79.8% | (many diffs) | |
| 4 | human | 122773 | KLHDC1 | kelch domain containing 1 | XR_001750145.1 | 44.1% | (many diffs) | |
| 5 | human | 122773 | KLHDC1 | kelch domain containing 1 | XM_024449471.1 | 32.1% | 34.4% | (many diffs) |
| 6 | human | 122773 | KLHDC1 | kelch domain containing 1 | XR_002957529.1 | 21.3% | (many diffs) | |
| 7 | human | 122773 | KLHDC1 | kelch domain containing 1 | XR_002957530.1 | 20.7% | (many diffs) | |
| 8 | mouse | 271005 | Klhdc1 | kelch domain containing 1 | NM_178253.5 | 88.3% | 19.9% | (many diffs) |
| 9 | mouse | 271005 | Klhdc1 | kelch domain containing 1 | XM_011244119.2 | 78.1% | 9.6% | (many diffs) |
| 10 | mouse | 271005 | Klhdc1 | kelch domain containing 1 | XM_011244120.1 | 78.1% | 9.6% | (many diffs) |
| 11 | mouse | 271005 | Klhdc1 | kelch domain containing 1 | XM_011244121.2 | 78.1% | 9.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 339
- ORF length:
- 270
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggactct cagctgttct gtgtggcgga ggaacgcagc ggccactgcg 121 ccgtggtgga cggaaacttc ctctacgtgt gggggggcta cgtgtctatt gaagacaatg 181 aagtatattt gcctaatgat gaaatttgga cctatgatat tgatagtggg ttgtggagaa 241 tgcacctcat ggaaggagaa ctcccagcct ccatgtcagg aagctgtggt gcttgcatta 301 atggaaagct gtacattttt ggaggatatg atgacaaatg atacagcaat cgactttatt 361 ttgttaattt acgaacaaga gatgaaacct acatttggga gaaaatcacc gactttgaag 421 ggcaaccacc tacaccacgt gataaacttt cctgctgggt atataaagac agactaatat 481 attttggtgg ttatgggtgt aggagacaca gtgaactcca agactgtttt gatgttcatg 541 atgcatcttg ggaagagcag atattctggg gatggcataa tgatgtccac atatttgaca 601 caaagacaca gacttggttt caaccagaaa ttaaaggtgg agttccacca cagccacgag 661 ccgcgcatac gtgtgcagtt cttggaaata agggttatat ctttggcgga cgtgttctgc 721 aaactaggat gaatgatttg cactatctaa acctagacac ctggacttgg tctggaagga 781 ttactattaa tggagaaagc ccaaaacatc ggtcatggca tactttaaca cctatagctg 841 atgataaact tttcctatgt ggtggactaa gtgcagataa tatcccatta agtgatggtt 901 ggattcataa tgtcacaaca aattgttgga aacaacttac acatttacct aaaacaagac 961 ctaggttatg gcacacagcc tgtttgggaa aagaaaatga aataatggta tttggtggga 1021 gcaaagatga cttacttgcc ttggatacag GTCACTGTAA TGATTTATTG ATCTTTCAAA 1081 CACAGCCTTA TTCACTACTC AGGTCATGCC TTGACTGCAT TGGTAAAAAT TCTATCATGT 1141 TAGAAAGTCA GATATCTTTA TTACCTCCTA AACTTCTGCA ACAAGTACTC AAAAAAATAA 1201 CATTTTGGGC TGCAGCTAAT CACCGAGAAG AACAAAGAGT CCAAAAAGAA GAAACAGAAA 1261 ATAAATATCA GTGGATCAGT AGCAATTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGGACGTC 1441 CTCTAATCAT GACTCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt