Construct: ORF TRCN0000476155
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017322.1_s317c1
- Derived from:
- ccsbBroadEn_05111
- DNA Barcode:
- AAACCCATGAGCAGATGGACCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D21 (161514)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476155
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | NM_153356.3 | 99.9% | 100% | 445C>T |
| 2 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | XM_011521283.2 | 98.2% | 97% | (many diffs) |
| 3 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | XM_011521282.2 | 97.4% | 95.3% | (many diffs) |
| 4 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | XM_011521281.3 | 92.3% | 92.2% | (many diffs) |
| 5 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | NM_001286434.2 | 89.1% | 89.2% | 59_60ins108;337C>T |
| 6 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | XM_006720409.4 | 83.2% | 82% | (many diffs) |
| 7 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | XM_006720410.4 | 74.2% | 73% | (many diffs) |
| 8 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | XM_024449857.1 | 70.4% | 70.5% | 0_1ins297;148C>T |
| 9 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | XM_024449856.1 | 64.9% | 64.8% | (many diffs) |
| 10 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | XM_011521284.3 | 61.1% | 60% | (many diffs) |
| 11 | human | 161514 | TBC1D21 | TBC1 domain family member 21 | XM_006720411.3 | 58.4% | 57.2% | (many diffs) |
| 12 | mouse | 74286 | Tbc1d21 | TBC1 domain family, member 21 | NM_028854.3 | 87% | 87.2% | (many diffs) |
| 13 | mouse | 74286 | Tbc1d21 | TBC1 domain family, member 21 | XR_379469.1 | 65.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1074
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac caccctctct cctgaaaaca gcctctctgc cagacagtca gcctccttca 121 tcctggtgaa gagaaaacca cccattgaca agacagaatg ggacagcttc tttgatgaga 181 gtggccactt ggccaaatca cgggacttca tttgtgttaa catcctggaa aggggtctgc 241 accccttcgt gaggactgaa gcctggaaat tcctcacggg ctacttctca tggcagagtt 301 cccaggatga gcggctcacg gtggacagca tgaggaggaa gaactacaag gccttatgcc 361 aaatgtatga gaagattcag ccccttctgg aaaacctgca ccggaacttc acagagactc 421 gcaataacat tgcacgtgac attcagaaaa tctatgacaa agatcccctg ggcaacgtcc 481 tcatcgacaa gaagaggcta gagaagatct tgctcctgag ttacgtctgc aacacgcagg 541 cagagtacca gcagggcttc catgagatga tgatgctctt ccagctgatg gtggagcacg 601 accacgagac cttctggctt ttccagttct tcctgcagaa aacggaacac agctgtgtca 661 tcaacattgg cgtggccaag aacctagaca tgctcagcac cctgatcacc ttcctggacc 721 ccgtgttTGC TGAGCACCTA AAAGGGAAGG GTGCAGGGGC TGTGCAGTCC CTCTTCCCCT 781 GGTTCTGCTT CTGCTTCCAG CGTGCCTTCA AGTCCTTCGA TGATGTCTGG AGGCTCTGGG 841 AGGTTCTGCT GACGGGGAAG CCCTGCAGGA ACTTCCAGGT GCTGGTGGCC TACAGCATGC 901 TGCAGATGGT GCGGGAGCAG GTGCTGCAGG AAAGCATGGG CGGGGATGAC ATCCTCCTGG 961 CCTGCAACAA CCTCATCGAC CTTGATGCTG ATGAGCTGAT CTCTGCCGCC TGCGTGGTTT 1021 ATGCTGAGCT CATCCAGAAG GATGTTCCTC AGACATTAAA GGATTTCTTC CTCTGCCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAA AACCCATGAG CAGATGGACC GCAACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att