Construct: ORF TRCN0000476219
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005109.1_s317c1
- Derived from:
- ccsbBroadEn_02140
- DNA Barcode:
- TGTTGGGCCGTAGAGCCTGTGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNHIT3 (9326)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476219
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9326 | ZNHIT3 | zinc finger HIT-type contai... | NM_004773.4 | 100% | 100% | |
| 2 | human | 9326 | ZNHIT3 | zinc finger HIT-type contai... | NM_001281432.2 | 68.2% | 64% | (many diffs) |
| 3 | human | 9326 | ZNHIT3 | zinc finger HIT-type contai... | NM_001281434.1 | 63.8% | 63.8% | 116_117ins168 |
| 4 | human | 9326 | ZNHIT3 | zinc finger HIT-type contai... | NM_001281433.1 | 51.6% | 43% | 203_204ins34;240_241ins191 |
| 5 | human | 9326 | ZNHIT3 | zinc finger HIT-type contai... | NR_104009.1 | 47.2% | 1_73del;189_190insAGAAC;534_969del | |
| 6 | human | 9326 | ZNHIT3 | zinc finger HIT-type contai... | NR_104010.1 | 44.4% | 1_73del;159_160ins32;507_942del | |
| 7 | human | 9326 | ZNHIT3 | zinc finger HIT-type contai... | NR_104011.2 | 19% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 534
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtcgctc aaatgtagca ccgtcgtctg cgtgatctgc ttggagaagc 121 ccaaataccg ctgtccagcc tgccgcgtgc ccTACTGCTC GGTAGTCTGC TTCCGGAAGC 181 ACAAAGAACA GTGCAACCCT GAAACTCGTC CTGTTGAGAA AAAAATAAGA TCAGCTCTTC 241 CTACCAAAAC CGTAAAGCCT GTGGAAAACA AAGATGATGA TGACTCTATA GCTGATTTTC 301 TCAATAGTGA TGAGGAAGAA GACAGAGTTT CTTTGCAGAA TTTAAAGAAT TTAGGGGAAT 361 CTGCAACATT AAGAAGCTTA TTGCTCAATC CACACCTCAG GCAGTTGATG GTCAACCTCG 421 ATCAGGGAGA AGACAAAGCA AAGCTCATGA GAGCTTACAT GCAAGAGCCT TTGTTTGTGG 481 AGTTTGCAGA CTGCTGTTTA GGAATTGTGG AGCCATCCCA GAATGAGGAG TCTTTGCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAT GTTGGGCCGT AGAGCCTGTG ACAACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att