Construct: ORF TRCN0000476256
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004909.1_s317c1
- Derived from:
- ccsbBroadEn_07381
- DNA Barcode:
- ATCCGTGCATGCCAGTGTACTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPKAPK2 (9261)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476256
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | NM_032960.4 | 100% | 100% | |
| 2 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | NM_004759.5 | 90.2% | 88.1% | (many diffs) |
| 3 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | XM_005273353.3 | 90.2% | 87.2% | (many diffs) |
| 4 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | XM_017002810.1 | 76.7% | 76.7% | 0_1ins279 |
| 5 | mouse | 17164 | Mapkapk2 | MAP kinase-activated protei... | NM_008551.2 | 86.3% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1266
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtccaactcc cagggccaga gcccgccggt gccgttcccc gccccggccc 121 cgccgccgca gccccccacc cctgccctgc cgcacccccc ggcgcagccg ccgccgccgc 181 ccccgcagca gttcccgcag ttccacgtca agtccggcct gcagatcaag aagaacgcca 241 tcatcgatga ctacaaggtc accagccagg tcctggggct gggcatcaac ggcaaagttt 301 tgcagatctt caacaagagg acccaggaga aattcgccct caaaatgctt caggactgcc 361 ccaaggcccg cagggaggtg gagctgcact ggcgggcctc ccagtgcccg cacatcgtac 421 ggatcgtgga tgtgtacgag aatctgtacg cagggaggaa gtgcctgctg attgtcatgg 481 aatgtttgga cggtggagaa ctctttagcc gaatccagga tcgaggagac caggcattca 541 cagaaagaga agcatccgaa atcatgaaga gcatcggtga ggccatccag tatctgcatt 601 caatcaacat tgcccatcgg gatgtcaagc ctgagaatct cttatacacc tccaaaaggc 661 ccaacgccat cctgaaactc actgactttg gctttgccaa ggaaaccacc agccacaact 721 ctttgaccac tccttgttat acaccgtact atgtggctcc agaagtgctg ggtccagaga 781 agtatgacaa gtcctgtgac atgtggtccc tgggtgtcat catgtacatc ctgctgtgtg 841 ggtatccccc cttctactcc aaccacggcc ttgccatctc tccgggcatg aagactcgca 901 TCCGAATGGG CCAGTATGAA TTTCCCAACC CAGAATGGTC AGAAGTATCA GAGGAAGTGA 961 AGATGCTCAT TCGGAATCTG CTGAAAACAG AGCCCACCCA GAGAATGACC ATCACCGAGT 1021 TTATGAACCA CCCTTGGATC ATGCAATCAA CAAAGGTCCC TCAAACCCCA CTGCACACCA 1081 GCCGGGTCCT GAAGGAGGAC AAGGAGCGGT GGGAGGATGT CAAGGAGGAG ATGACCAGTG 1141 CCTTGGCCAC AATGCGCGTT GACTACGAGC AGATCAAGAT AAAAAAGATT GAAGATGCAT 1201 CCAACCCTCT GCTGCTGAAG AGGCGGAAGA AAGCTCGGGC CCTGGAGGCT GCGGCTCTGG 1261 CCCACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCCGTGCA TGCCAGTGTA CTACAACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt