Construct: ORF TRCN0000476256

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004909.1_s317c1
Derived from:
ccsbBroadEn_07381
DNA Barcode:
ATCCGTGCATGCCAGTGTACTACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAPKAPK2 (9261)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476256
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9261 MAPKAPK2 MAPK activated protein kina... NM_032960.4 100% 100%
2 human 9261 MAPKAPK2 MAPK activated protein kina... NM_004759.5 90.2% 88.1% (many diffs)
3 human 9261 MAPKAPK2 MAPK activated protein kina... XM_005273353.3 90.2% 87.2% (many diffs)
4 human 9261 MAPKAPK2 MAPK activated protein kina... XM_017002810.1 76.7% 76.7% 0_1ins279
5 mouse 17164 Mapkapk2 MAP kinase-activated protei... NM_008551.2 86.3% 92.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1266
ORF length:
1200
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gtccaactcc cagggccaga gcccgccggt gccgttcccc gccccggccc
121cgccgccgca gccccccacc cctgccctgc cgcacccccc ggcgcagccg ccgccgccgc
181ccccgcagca gttcccgcag ttccacgtca agtccggcct gcagatcaag aagaacgcca
241tcatcgatga ctacaaggtc accagccagg tcctggggct gggcatcaac ggcaaagttt
301tgcagatctt caacaagagg acccaggaga aattcgccct caaaatgctt caggactgcc
361ccaaggcccg cagggaggtg gagctgcact ggcgggcctc ccagtgcccg cacatcgtac
421ggatcgtgga tgtgtacgag aatctgtacg cagggaggaa gtgcctgctg attgtcatgg
481aatgtttgga cggtggagaa ctctttagcc gaatccagga tcgaggagac caggcattca
541cagaaagaga agcatccgaa atcatgaaga gcatcggtga ggccatccag tatctgcatt
601caatcaacat tgcccatcgg gatgtcaagc ctgagaatct cttatacacc tccaaaaggc
661ccaacgccat cctgaaactc actgactttg gctttgccaa ggaaaccacc agccacaact
721ctttgaccac tccttgttat acaccgtact atgtggctcc agaagtgctg ggtccagaga
781agtatgacaa gtcctgtgac atgtggtccc tgggtgtcat catgtacatc ctgctgtgtg
841ggtatccccc cttctactcc aaccacggcc ttgccatctc tccgggcatg aagactcgca
901TCCGAATGGG CCAGTATGAA TTTCCCAACC CAGAATGGTC AGAAGTATCA GAGGAAGTGA
961AGATGCTCAT TCGGAATCTG CTGAAAACAG AGCCCACCCA GAGAATGACC ATCACCGAGT
1021TTATGAACCA CCCTTGGATC ATGCAATCAA CAAAGGTCCC TCAAACCCCA CTGCACACCA
1081GCCGGGTCCT GAAGGAGGAC AAGGAGCGGT GGGAGGATGT CAAGGAGGAG ATGACCAGTG
1141CCTTGGCCAC AATGCGCGTT GACTACGAGC AGATCAAGAT AAAAAAGATT GAAGATGCAT
1201CCAACCCTCT GCTGCTGAAG AGGCGGAAGA AAGCTCGGGC CCTGGAGGCT GCGGCTCTGG
1261CCCACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1321TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1381CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCCGTGCA TGCCAGTGTA CTACAACGCG
1441TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)