Construct: ORF TRCN0000476280
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015532.1_s317c1
- Derived from:
- ccsbBroadEn_10069
- DNA Barcode:
- GCTCAAACTTGTACCCCAGTTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CERKL (375298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476280
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375298 | CERKL | ceramide kinase like | NM_201548.5 | 99.9% | 100% | 1506C>T |
| 2 | human | 375298 | CERKL | ceramide kinase like | NM_001030311.2 | 95.2% | 95.3% | 677_754del;1584C>T |
| 3 | human | 375298 | CERKL | ceramide kinase like | NM_001160277.1 | 87.3% | 87.2% | 481_482ins132;545_622del;1452C>T |
| 4 | human | 375298 | CERKL | ceramide kinase like | NM_001030313.2 | 86.9% | 86.8% | 613_614ins207;1299C>T |
| 5 | human | 375298 | CERKL | ceramide kinase like | NM_001030312.2 | 78.6% | 78.7% | 477_478ins339;1167C>T |
| 6 | human | 375298 | CERKL | ceramide kinase like | NR_027690.1 | 47.4% | (many diffs) | |
| 7 | human | 375298 | CERKL | ceramide kinase like | NR_027689.1 | 43.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1665
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccctggagg aggcgcagga accgggtgag tgccctggag ggcggccggg 121 aggaagaggc gcccccggag gctgccgctg tgcctccggc gctgttaacg tccccgcagc 181 agacggaggc ggcggccgag cggattctgc tccggggcat cttcgagatc gggagggaca 241 gttgtgacgt ggtgctgagc gagcgagcac tgcggtggcg gcccattcag cccgagcgcc 301 cggcgggtga ttctaagtat gacttgctat gtaaagaaga atttattgaa ctcaaagaca 361 tattctctgt gaaactgaaa cggcgttgtt ctgttaaaca gcagagaagt ggtactttat 421 taggtatcac actcttcatc tgcttgaaaa aggaacaaaa taaactaaag aattctacac 481 ttgatcttat taatttaagt gaagaccact gtgacatatg gtttagacag ttcaagaaaa 541 tattggcagg ctttccaaac agaccgaagt cattaaaaat actccttaac ccccaaagtc 601 acaaaaaaga agctacccag gtttattatg agaaggttga acctctgttg aagcttgcag 661 gaataaaaac tgatgtaaca ataatggaat atgaagggca cgctctgtca ctgcttaagg 721 aatgtgaact ccagggattt gatggtgttg tctgtgttgg tggagatgga tctgctagcg 781 aagtagccca tgctttgctt ctgagagctc agaagaatgc tgggatggaa acagaccgaa 841 tcctgactcc tgtcagagca cagcttccac ttggcttaat accagcagga tctaccaatg 901 tattggcaca ttctcttcat ggagttcctc atgtgataac tgcaacattg cacattataa 961 tggggcatgt acagctggtc gacgtctgca ccttcagcac cgctggcaag cttcttcgct 1021 ttgggttctc agccatgttt ggctttggtg gaagaacttt ggctctggca gaaaaatatc 1081 gatggatgtc ccctaaccaa cggagagatt ttgctgttgt taaggcactg gcaaaactta 1141 aggcagaaga ctgtgaaata tcatttttac catttaacag ctctgatgat gtgcaagaaa 1201 ggagggcaca gggatctccc aaatctgact gtaatgatca atggcaaatg atccagggtc 1261 agttcttgaa tgtcagcatt atggcaattc cttgcctgtg ttcagtggca cctagaggct 1321 tggcacctaa taccagatta aataatggaa gtatggctct tataattgcc cgaaacactt 1381 cTCGGCCAGA ATTTATAAAA CACCTGAAAA GATATGCCAG TGTAAAAAAT CAGTTCAATT 1441 TTCCATTTGT TGAGACTTAC ACTGTTGAGG AAGTAAAAGT TCATCCAAGG AATAATACTG 1501 GTGGATATAA TCCAGAGGAG GAGGAGGATG AAACTGCTTC AGAAAATTGT TTCCCTTGGA 1561 ATGTAGATGG TGATTTAATG GAAGTTGCAT CAGAGGTCCA TATTAGATTG CATCCAAGAC 1621 TTATCAGTCT TTATGGAGGA AGCATGGAAG AAATGATTCC AAAGTTGCCA ACTTTCTTGT 1681 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1741 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1801 GAAAGGACGA GCTCAAACTT GTACCCCAGT TATAACGCGT TAAGTCgaca atcaacctct 1861 ggattacaaa atttgtgaaa gatt