Construct: ORF TRCN0000476300
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007957.1_s317c1
- Derived from:
- ccsbBroadEn_06744
- DNA Barcode:
- GATTGACAGTGTAAACATATTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXOSC9 (5393)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476300
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5393 | EXOSC9 | exosome component 9 | NM_005033.3 | 99.9% | 99.7% | 607A>G |
| 2 | human | 5393 | EXOSC9 | exosome component 9 | NM_001034194.2 | 96.1% | 96% | 607A>G;1155_1205del |
| 3 | human | 5393 | EXOSC9 | exosome component 9 | XM_011532035.3 | 88.7% | 88.1% | (many diffs) |
| 4 | human | 5393 | EXOSC9 | exosome component 9 | XR_001741241.2 | 79.1% | (many diffs) | |
| 5 | mouse | 50911 | Exosc9 | exosome component 9 | NM_019393.2 | 87.9% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1386
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaggaaacg ccactctcaa actgcgaacg ccgcttccta ctccgtgcca 121 tcgaagagaa gaagcggctg gatggcagac aaacctatga ttataggaac atcaggatct 181 catttggaac agattacgga tgctgcattg tggaacttgg aaaaacaaga gttcttggac 241 aggtttcctg tgaacttgtg tctccaaaac tcaatcgggc aacagaaggt attctttttt 301 ttaaccttga actctctcag atggccgctc cagctttcga acctggcagg cagtcagatc 361 tcttggtgaa gttgaatcga ctcatggaaa gatgtctaag aaattcgaag tgtatagaca 421 ctgagtctct ctgtgttgtt gctggtgaaa aggtttggca aatacgtgta gacctacatt 481 tattaaatca tgatggaaat attattgatg ctgccagcat tgctgcaatc gtggccttat 541 gtcatttccg aagacctgat gtctctgtcc aaggagatga agtaacactg tatacacctg 601 aagagcgtga tcctgtacca ttaagtatcc accacatgcc catttgtgtc agttttgcct 661 ttttccagca aggagcatat ttattggtgg atcccaatga acgagaagaa cgtgtgatgg 721 atggcttgct ggtgattgcc atgaacaaac atcgagagat ttgtactatc cagtccagtg 781 gtgggataat gctactaaaa gatcaagttc tgagatgcag taaaatcgct ggtgtgaaag 841 tagcagaaat tacagagcta atattgaaag ctttggagaa tgaccaaaaa gtaaggaaag 901 aaggtggaaa gtttggtttt gcagagtcta tagcaaatca aaggatcaca gcatttaaaa 961 tggaaaaggc ccctattgat acctcggatg tagaagaaaa agcagaagaa atcattgctg 1021 aagcagaacc TCCTTCAGAA GTTGTTTCTA CACCTGTGCT ATGGACTCCT GGAACTGCCC 1081 AAATTGGAGA GGGAGTAGAA AACTCCTGGG GTGATCTTGA AGACTCTGAG AAGGAAGATG 1141 ATGAAGGCGG TGGTGATCAA GCTATCATTC TTGATGGTAT AAAAATGGAC ACTGGAGTAG 1201 AAGTCTCTGA TATTGGAAGC CAAGATGCTC CCATAATACT CTCAGATAGT GAAGAAGAAG 1261 AAATGATCAT TTTGGAACCA GACAAGAATC CAAAGAAAAT AAGAACACAG ACCACCAGTG 1321 CAAAACAAGA AAAAGCACCA AGTAAAAAGC CAGTGAAAAG AAGAAAAAAG AAGAGAGCTG 1381 CCAATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGATTGACAG TGTAAACATA TTTCCACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt