Construct: ORF TRCN0000476356
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016472.1_s317c1
- Derived from:
- ccsbBroadEn_10137
- DNA Barcode:
- ACCTAACCGTGAAGTCACCGCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XKRX (402415)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476356
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 402415 | XKRX | XK related X-linked | NM_212559.3 | 97.1% | 97.1% | 0_1ins39 |
| 2 | human | 402415 | XKRX | XK related X-linked | XM_011530954.3 | 81.3% | 78.7% | (many diffs) |
| 3 | human | 402415 | XKRX | XK related X-linked | XM_011530955.1 | 72% | 72% | 0_1ins387 |
| 4 | human | 402415 | XKRX | XK related X-linked | XM_005262130.3 | 53% | 53% | 0_1ins651 |
| 5 | human | 402415 | XKRX | XK related X-linked | XM_017029517.1 | 53% | 53% | 0_1ins651 |
| 6 | mouse | 331524 | Xkrx | X-linked Kx blood group rel... | NM_183319.2 | 88.2% | 89.3% | (many diffs) |
| 7 | mouse | 331524 | Xkrx | X-linked Kx blood group rel... | XM_006528568.3 | 68.6% | 68.8% | (many diffs) |
| 8 | mouse | 331524 | Xkrx | X-linked Kx blood group rel... | XM_011247748.2 | 68.6% | 68.8% | (many diffs) |
| 9 | mouse | 331524 | Xkrx | X-linked Kx blood group rel... | XM_017318525.1 | 68.6% | 68.8% | (many diffs) |
| 10 | mouse | 331524 | Xkrx | X-linked Kx blood group rel... | XM_006528569.3 | 65.3% | 65.5% | (many diffs) |
| 11 | mouse | 331524 | Xkrx | X-linked Kx blood group rel... | XM_006528570.3 | 47.4% | 47.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1455
- ORF length:
- 1386
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacacaaga ccacaacatt cagaaagaac ctcgacaatg gacagagttt 121 atgaaattcc tgaggagcca aatgtggatc cggtttcatc tctggaggaa gatgtcatcc 181 gtggagccaa cccccgattt acttttccat ttagcatcct tttctccacc tttttgtact 241 gtggggaggc tgcatctgct ttgtacatgg ttagaatcta tcgaaagaat agtgaaactt 301 actggatgac atacaccttt tctttcttta tgttttcatc cattatggtc cagttgaccc 361 tcatttttgt ccacagagat ctagccaaag ataaaccgct atcattattt atgcatctaa 421 tcctcttggg acctgttatc agatgtttgg aggccatgat taagtacctc acactgtgga 481 agaaagagga gcaggaggag ccctatgtca gcctcacccg aaagaagatg ctaatagatg 541 gcgaggaggt gctgatagaa tgggaggtgg gccactccat ccggaccctg gctatgcacc 601 gcaatgccta caaacgtatg tcacagatcc aagccttcct gggctcagtg ccccagctga 661 cctatcagct ctatgtgagc ctgatctctg cagaggttcc cctgggtaga gttgtgctaa 721 tggtattttc cctggtatct gtcacctatg gggccaccct ttgcaatatg ttggctatcc 781 agatcaagta cgatgactac aagattcgcc ttgggccact agaagtcctc tgcatcacca 841 tctggcggac attggagatc acttcccgcc tcctgattct ggtgctcttc tcagccactt 901 tgaaattgaa ggctgtgccc ttcctagtgc tcaacttcct gatcatcctc tttgagccct 961 ggattaagtt ctggagaagt ggtgcccaga tgcccaataa cattgagaaa aacttcagcc 1021 gggtcggcac tctggtggtc ctgatttcag tcaccatcct ctatgctggc atcaacttct 1081 cttgctggtc agctttgcag ttgaggttgg cagacagaga TCTCGTCGAC AAAGGGCAGA 1141 ACTGGGGACA TATGGGCCTG CACTATAGTG TGAGGTTGGT AGAGAATGTG ATCATGGTCT 1201 TGGTTTTTAA GTTCTTTGGA GTGAAAGTGT TACTGAATTA CTGTCATTCC TTGATTGCCT 1261 TGCAGCTCAT TATTGCTTAT CTGATTTCCA TTGGCTTCAT GCTCCTTTTC TTCCAGTACT 1321 TGCATCCATT GCGCTCACTC TTCACCCATA ATGTAGTAGA CTACCTCCAT TGTGTCTGCT 1381 GTCACCAGCA CCCTCGGACC AGGGTTGAGA ACTCAGAGCC ACCCTTTGAG ACTGAAGCAA 1441 GGCAAAGTGT TGTCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1501 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1561 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACCTAACCGT GAAGTCACCG 1621 CACCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt