Construct: ORF TRCN0000476364
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015648.1_s317c1
- Derived from:
- ccsbBroadEn_13686
- DNA Barcode:
- GATATCAAAAAACTAAAAACATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM41B (440026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476364
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440026 | TMEM41B | transmembrane protein 41B | NM_001165030.3 | 65.2% | 64% | (many diffs) |
| 2 | human | 440026 | TMEM41B | transmembrane protein 41B | NM_015012.4 | 64.6% | 43.9% | 368_369insTTACCAG;570_873del |
| 3 | human | 440026 | TMEM41B | transmembrane protein 41B | NR_028491.3 | 38.5% | 1_152del;520_521insTTACCAG;722_1469del | |
| 4 | human | 440026 | TMEM41B | transmembrane protein 41B | XR_930870.1 | 37.9% | 1_192del;769_1516del | |
| 5 | human | 440026 | TMEM41B | transmembrane protein 41B | XR_001747881.1 | 14.9% | 1_192del;769_3845del | |
| 6 | mouse | 233724 | Tmem41b | transmembrane protein 41B | NM_153525.5 | 55.6% | 36.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 645
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgaaaggc agagtcgccg aacgatcgca gttgggcgct caccacacga 121 cccccgtggg ggacggggca gcggggacgc ggggtctcgc ggcgcctggc agcagagacc 181 accagaagga aaaatcctgg gtagaagctg gatcagcaag aatgtcactc cttatattgg 241 tgtccatttt cttatctgca gcttttgtta tgtttttggt atataaaaat tttccTCAGC 301 TTAGTGAAGA AGAAAGAGTG AATATGAAGG TTCCCAGAGA TATGGATGAT GCCAAGGCTC 361 TAGGAAAAGT TTTATCCAAA TACAAGGACA CCTTTTATGT TCAAGTACTT GTAGCTTATT 421 TTGCTACATA TATTTTTTAC CAGCTTGCAA ACATTTGCTA TTCCAGGCTC TATATTTCTC 481 AGTATACTCT CAGGGTTTCT TTATCCCTTT CCACTAGCCT TATTTCTTGT TTGTTTGTGT 541 TCTGGACTTG GTGCCTCTTT CTGTTATATG CTTTCCTATT TAGTTGGGAG ACCAGTTGTA 601 TACAAATACC TAACAGAGAA AGCAGTAAAA TGGTCACAGC AGGTTTGCCA ACTTTCTTGT 661 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 721 AGTAATGAAC TAGCCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 781 GAAAGGACGA GATATCAAAA AACTAAAAAC ATCTACGCGT TAAGTCgaca atcaacctct 841 ggattacaaa atttgtgaaa gatt