Construct: ORF TRCN0000476383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004626.1_s317c1
- Derived from:
- ccsbBroadEn_11675
- DNA Barcode:
- AATCGAACAGTCTTACTCGTTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCF2L2 (23101)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476383
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23101 | MCF2L2 | MCF.2 cell line derived tra... | XM_017005944.2 | 89% | 87.9% | (many diffs) |
| 2 | human | 23101 | MCF2L2 | MCF.2 cell line derived tra... | XM_017005945.2 | 87.9% | 87.2% | (many diffs) |
| 3 | human | 23101 | MCF2L2 | MCF.2 cell line derived tra... | XM_017005946.2 | 87.9% | 87.2% | (many diffs) |
| 4 | human | 23101 | MCF2L2 | MCF.2 cell line derived tra... | XM_017005943.2 | 72.1% | 71.6% | (many diffs) |
| 5 | human | 23101 | MCF2L2 | MCF.2 cell line derived tra... | NM_015078.3 | 63.5% | 63.1% | (many diffs) |
| 6 | human | 23101 | MCF2L2 | MCF.2 cell line derived tra... | XM_011512585.2 | 31.9% | 31.5% | (many diffs) |
| 7 | human | 23101 | MCF2L2 | MCF.2 cell line derived tra... | XM_011512586.2 | 31.9% | 31.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2199
- ORF length:
- 2133
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtcttgctta aaagaagaga tgcctcccca ggagctcacc cggcgactgg 121 ccacagtgat cactcatgtc gatgaaatta tgcagcagga agtcagaccc ctgatggcgg 181 tggagataat agaacaactt cacagacaat ttgccattct ttcaggaggc cgaggggagg 241 atggcgcccc catcatcacg ttcccagagt tttcggggtt caaacacatc ccagatgaag 301 acttcctgaa tgtcatgacc tacctgacta gcatccccag tgtggaggct gccagcattg 361 gattcattgt tgttatcgac agacgaagag acaagtggag ctccgtaaag gcatccttga 421 cacgaatagc tgtggcattt ccaggaaact tacagctcat attcatcctt cgtccatctc 481 gctttatcca gaggacattc actgacattg gcattaaata ctatcgaaat gagtttaaaa 541 cgaaagtgcc gatcatcatg gtaaactctg tctctgacct tcacggctac atcgacaaaa 601 gccaactgac ccgggaatta ggggggactt tggaatatcg ccacggtcag tgggtaaatc 661 accgcactgc catcgaaaac tttgccttga ccttgaagac cactgcccag atgctgcaga 721 cgtttgggtc ctgcctggcc acagcagagc tgcccagaag catgctatcc acggaagacc 781 ttctcatgtc ccacacaagg cagcgggaca agctgcagga tgagctgaaa ttacttggaa 841 agcaggggac cacattgctg tcatgcatcc aagaaccagc aaccaaatgt cccaacagca 901 aactcaatct caaccaactt gagaatgtaa ctaccatgga aaggttatta gttcaactgg 961 atgaaacaga aaaagccttt agtcactttt ggtctgagca tcatctgaag cttaaccagt 1021 gcctacaact acagcatttt gagcacgatt tttgtaaggc taagcttgcc ctggataatt 1081 tgctggaaga gcaagcagag tttacaggca ttggagacag cgtgatgcac gtggagcaga 1141 ttcttaagga acacaaaaaa ctggaggaaa aaagccagga gcccctggaa aaggccctgc 1201 tgctggcact ggttggggac cagctcatcc aaagccacca ttatgcagca gatgccatca 1261 ggccccggtg tgtggagctc aggcacctct gtgacgattt catcaatgga aacaagaaaa 1321 aatgggacat tttaggaaag tccttagagt ttcatagaca gctggacaag gtcagccaat 1381 ggtgtgaggc aggaatctac ctcttggctt cccaagctgt agacaagtgc cagtctcgag 1441 aaggggttga tatcgccttg aacgacattg cgacattcct gggcacagtc aaggagtacc 1501 cgttgctcag ccccaaggag ttttacaacg agtttgagtt gctgctcacc ctcgatgcaa 1561 aggccaaagc ccagaaagtt ttgcagaggc tggatgatgt ccaggaaata tttcacaaga 1621 ggcaagtgag tctgatgaaa ctggcagcca aacagactcg tccagtgcaa cctgtggccc 1681 cacatcctga gtcttcacca aaatgggtgt catcaaaaac cagccagccc tccacctcgg 1741 tccctctagc tcgtcctctg agaacgtctg aggaacctta tacggagaca gagttgaact 1801 cccgggGAAA GGAAGATGAT GAGACTAAAT CTGAAGTCAA GAGTGAAGAA ATCTTTGAAA 1861 GCCATCATGA AAGGGGGAAC CCTGAGCTGG AGCAGCAGGC CAGGCTCGGA GACCTTTCCC 1921 CCCGCAGGCG CATTATACGT GACTTGCTTG AGACTGAAGA GATTTACATA AAAGAGATTA 1981 AAAGCATAAT TGATGGATAT ATCACTCCAA TGGATTTTAT TTGGCTAAAG CATCTAATTC 2041 CAGATGTTCT TCAGAATAAC AAGGACTTTC TCTTTGGGAA TATTAGAGAA CTTTACGAAT 2101 TTCACAACAG GACTTTTCTA AAAGAGTTGG AAAAGTGTGC TGAGAACCCT GAACTTCTGG 2161 CACATTGCTT TCTCAAGAGA GTAAGTCTGT ACTCCCAGTA CCCAACTTTC TTGTACAAAG 2221 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2281 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2341 ACGAAATCGA ACAGTCTTAC TCGTTTCGAC GCGTTAAGTC gacaatcaac ctctggatta 2401 caaaatttgt gaaagatt