Construct: ORF TRCN0000476383

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004626.1_s317c1
Derived from:
ccsbBroadEn_11675
DNA Barcode:
AATCGAACAGTCTTACTCGTTTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCF2L2 (23101)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476383
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23101 MCF2L2 MCF.2 cell line derived tra... XM_017005944.2 89% 87.9% (many diffs)
2 human 23101 MCF2L2 MCF.2 cell line derived tra... XM_017005945.2 87.9% 87.2% (many diffs)
3 human 23101 MCF2L2 MCF.2 cell line derived tra... XM_017005946.2 87.9% 87.2% (many diffs)
4 human 23101 MCF2L2 MCF.2 cell line derived tra... XM_017005943.2 72.1% 71.6% (many diffs)
5 human 23101 MCF2L2 MCF.2 cell line derived tra... NM_015078.3 63.5% 63.1% (many diffs)
6 human 23101 MCF2L2 MCF.2 cell line derived tra... XM_011512585.2 31.9% 31.5% (many diffs)
7 human 23101 MCF2L2 MCF.2 cell line derived tra... XM_011512586.2 31.9% 31.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2199
ORF length:
2133
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gtcttgctta aaagaagaga tgcctcccca ggagctcacc cggcgactgg
121ccacagtgat cactcatgtc gatgaaatta tgcagcagga agtcagaccc ctgatggcgg
181tggagataat agaacaactt cacagacaat ttgccattct ttcaggaggc cgaggggagg
241atggcgcccc catcatcacg ttcccagagt tttcggggtt caaacacatc ccagatgaag
301acttcctgaa tgtcatgacc tacctgacta gcatccccag tgtggaggct gccagcattg
361gattcattgt tgttatcgac agacgaagag acaagtggag ctccgtaaag gcatccttga
421cacgaatagc tgtggcattt ccaggaaact tacagctcat attcatcctt cgtccatctc
481gctttatcca gaggacattc actgacattg gcattaaata ctatcgaaat gagtttaaaa
541cgaaagtgcc gatcatcatg gtaaactctg tctctgacct tcacggctac atcgacaaaa
601gccaactgac ccgggaatta ggggggactt tggaatatcg ccacggtcag tgggtaaatc
661accgcactgc catcgaaaac tttgccttga ccttgaagac cactgcccag atgctgcaga
721cgtttgggtc ctgcctggcc acagcagagc tgcccagaag catgctatcc acggaagacc
781ttctcatgtc ccacacaagg cagcgggaca agctgcagga tgagctgaaa ttacttggaa
841agcaggggac cacattgctg tcatgcatcc aagaaccagc aaccaaatgt cccaacagca
901aactcaatct caaccaactt gagaatgtaa ctaccatgga aaggttatta gttcaactgg
961atgaaacaga aaaagccttt agtcactttt ggtctgagca tcatctgaag cttaaccagt
1021gcctacaact acagcatttt gagcacgatt tttgtaaggc taagcttgcc ctggataatt
1081tgctggaaga gcaagcagag tttacaggca ttggagacag cgtgatgcac gtggagcaga
1141ttcttaagga acacaaaaaa ctggaggaaa aaagccagga gcccctggaa aaggccctgc
1201tgctggcact ggttggggac cagctcatcc aaagccacca ttatgcagca gatgccatca
1261ggccccggtg tgtggagctc aggcacctct gtgacgattt catcaatgga aacaagaaaa
1321aatgggacat tttaggaaag tccttagagt ttcatagaca gctggacaag gtcagccaat
1381ggtgtgaggc aggaatctac ctcttggctt cccaagctgt agacaagtgc cagtctcgag
1441aaggggttga tatcgccttg aacgacattg cgacattcct gggcacagtc aaggagtacc
1501cgttgctcag ccccaaggag ttttacaacg agtttgagtt gctgctcacc ctcgatgcaa
1561aggccaaagc ccagaaagtt ttgcagaggc tggatgatgt ccaggaaata tttcacaaga
1621ggcaagtgag tctgatgaaa ctggcagcca aacagactcg tccagtgcaa cctgtggccc
1681cacatcctga gtcttcacca aaatgggtgt catcaaaaac cagccagccc tccacctcgg
1741tccctctagc tcgtcctctg agaacgtctg aggaacctta tacggagaca gagttgaact
1801cccgggGAAA GGAAGATGAT GAGACTAAAT CTGAAGTCAA GAGTGAAGAA ATCTTTGAAA
1861GCCATCATGA AAGGGGGAAC CCTGAGCTGG AGCAGCAGGC CAGGCTCGGA GACCTTTCCC
1921CCCGCAGGCG CATTATACGT GACTTGCTTG AGACTGAAGA GATTTACATA AAAGAGATTA
1981AAAGCATAAT TGATGGATAT ATCACTCCAA TGGATTTTAT TTGGCTAAAG CATCTAATTC
2041CAGATGTTCT TCAGAATAAC AAGGACTTTC TCTTTGGGAA TATTAGAGAA CTTTACGAAT
2101TTCACAACAG GACTTTTCTA AAAGAGTTGG AAAAGTGTGC TGAGAACCCT GAACTTCTGG
2161CACATTGCTT TCTCAAGAGA GTAAGTCTGT ACTCCCAGTA CCCAACTTTC TTGTACAAAG
2221TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
2281GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
2341ACGAAATCGA ACAGTCTTAC TCGTTTCGAC GCGTTAAGTC gacaatcaac ctctggatta
2401caaaatttgt gaaagatt

Download FASTA (ORF) (Full)