Construct: ORF TRCN0000476438
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013711.1_s317c1
- Derived from:
- ccsbBroadEn_07497
- DNA Barcode:
- CCGCGCCCGGTTAGCCATATAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA0319 (9856)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476438
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9856 | KIAA0319 | KIAA0319 | NM_001168375.2 | 99.9% | 99.8% | 424A>C;2618G>A |
| 2 | human | 9856 | KIAA0319 | KIAA0319 | NM_001350403.2 | 99.9% | 99.8% | 424A>C;2618G>A |
| 3 | human | 9856 | KIAA0319 | KIAA0319 | NM_014809.4 | 99.9% | 99.8% | 424A>C;2618G>A |
| 4 | human | 9856 | KIAA0319 | KIAA0319 | NM_001350404.2 | 99% | 97.7% | (many diffs) |
| 5 | human | 9856 | KIAA0319 | KIAA0319 | NM_001168374.1 | 98.4% | 97.7% | (many diffs) |
| 6 | human | 9856 | KIAA0319 | KIAA0319 | XM_017011541.1 | 98.4% | 97.7% | (many diffs) |
| 7 | human | 9856 | KIAA0319 | KIAA0319 | NM_001350405.2 | 96.8% | 96.7% | 424A>C;992_993ins99;2519G>A |
| 8 | human | 9856 | KIAA0319 | KIAA0319 | XM_017011544.1 | 96.8% | 96.7% | 424A>C;992_993ins99;2519G>A |
| 9 | human | 9856 | KIAA0319 | KIAA0319 | XM_017011542.2 | 96% | 95.4% | (many diffs) |
| 10 | human | 9856 | KIAA0319 | KIAA0319 | NM_001168376.1 | 95.7% | 95.6% | 0_1ins135;289A>C;2483G>A |
| 11 | human | 9856 | KIAA0319 | KIAA0319 | NM_001350406.2 | 95.7% | 95.6% | 0_1ins135;289A>C;2483G>A |
| 12 | human | 9856 | KIAA0319 | KIAA0319 | XM_011515026.3 | 95.7% | 95.6% | 0_1ins135;289A>C;2483G>A |
| 13 | human | 9856 | KIAA0319 | KIAA0319 | NM_001168377.1 | 94.2% | 94.1% | 424A>C;2618G>A;2855_2856ins183 |
| 14 | human | 9856 | KIAA0319 | KIAA0319 | NM_001350407.2 | 91.1% | 86.9% | (many diffs) |
| 15 | human | 9856 | KIAA0319 | KIAA0319 | NM_001350408.2 | 91.1% | 86.9% | (many diffs) |
| 16 | human | 9856 | KIAA0319 | KIAA0319 | XM_017011547.1 | 91.1% | 86.9% | (many diffs) |
| 17 | human | 9856 | KIAA0319 | KIAA0319 | XM_017011550.1 | 91.1% | 86.9% | (many diffs) |
| 18 | human | 9856 | KIAA0319 | KIAA0319 | XM_017011546.2 | 90.9% | 88.7% | (many diffs) |
| 19 | human | 9856 | KIAA0319 | KIAA0319 | XM_017011551.1 | 87.3% | 85.3% | (many diffs) |
| 20 | human | 9856 | KIAA0319 | KIAA0319 | NM_001350409.2 | 85.7% | 85.7% | 0_1ins456;2162G>A |
| 21 | human | 9856 | KIAA0319 | KIAA0319 | NM_001350410.2 | 85.7% | 85.7% | 0_1ins456;2162G>A |
| 22 | human | 9856 | KIAA0319 | KIAA0319 | XR_001743779.1 | 49.1% | (many diffs) | |
| 23 | human | 9856 | KIAA0319 | KIAA0319 | XR_001743780.1 | 47.7% | (many diffs) | |
| 24 | human | 9856 | KIAA0319 | KIAA0319 | NM_001252328.1 | 45% | 44.9% | 0_1ins1767;851G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3285
- ORF length:
- 3216
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgcccccc acaggtgtgc tctcttcatt gctgctgctg gtgacaattg 121 caggttgtgc ccgtaagcag tgcagcgagg ggaggacata ttccaatgca gtcatttcac 181 ctaacttgga aaccaccaga atcatgcggg tgtctcacac cttccctgtc gtagactgca 241 cggccgcttg ctgtgacctg tccagctgtg acctggcctg gtggttcgag ggccgctgct 301 acctggtgag ctgcccccac aaagagaact gtgagcccaa gaagatgggc cccatcaggt 361 cttatctcac ttttgtgctc cggcctgttc agaggcctgc acagctgctg gactatgggg 421 acatgatgct gaacaggggc tccccctcgg ggatctgggg ggactcacct gaggatatca 481 gaaaggactt gccctttcta ggcaaagatt ggggcctaga ggagatgtct gagtactcag 541 atgactaccg ggagctggag aaggacctct tgcaacccag tggcaagcag gagcccagag 601 ggagtgccga gtacacggac tggggcctac tgccgggcag cgagggggcc ttcaactcct 661 ctgttggaga cagtcctgcg gtgccagcgg agacgcagca ggaccctgag ctccattacc 721 tgaatgagtc ggcttcaacc cctgccccaa aactccctga gagaagtgtg ttgcttccct 781 tgccgactac tccatcttca ggagaggtgt tggagaaaga aaaggcttct cagctccagg 841 aacaatccag caacagctct ggaaaagagg ttctaatgcc ttcccatagt cttcctccgg 901 caagcctgga gctcagctca gtcaccgtgg agaaaagccc agtgctcaca gtcaccccgg 961 ggagtacaga gcacagcatc ccaacacctc ccactagcgc agccccctct gagtccaccc 1021 catctgagct acccatatct cctaccactg ctcccaggac agtgaaagaa cttacggtat 1081 cggctggaga taacctaatt ataactttac ccgacaatga agttgaactg aaggcctttg 1141 ttgcgccagc gccacctgta gaaacaacct acaactatga atggaattta ataagccacc 1201 ccacagacta ccaaggtgaa ataaaacaag gacacaagca aactcttaac ctctctcaat 1261 tgtccgtcgg actttatgtc ttcaaagtca ctgtttctag tgaaaacgcc tttggagaag 1321 gatttgtcaa tgtcactgtt aagcctgcca gaagagtcaa cctgccacct gtagcagttg 1381 tttctcccca actgcaagag ctcactttgc ctttgacgtc agccctcatt gatggcagcc 1441 aaagtacaga tgatactgaa atagtgagtt atcattggga agaaataaac gggcccttca 1501 tagaagagaa gacttcagtt gactctcccg tcttacgctt gtctaacctt gatcctggta 1561 actatagttt caggttgact gttacagact cggacggagc cactaactct acaactgcag 1621 ccctaatagt gaacaatgct gtggactacc caccagttgc taatgcagga ccaaatcaca 1681 ccataacttt gccccaaaac tccatcactt tgaatggaaa ccagagcagt gacgatcacc 1741 agattgtcct ctatgagtgg tccctgggtc ctgggagtga gggcaaacat gtggtcatgc 1801 agggagtaca gacgccatac cttcatttat ctgcaatgca ggaaggagat tatacatttc 1861 agctgaaggt gacagattct tcaaggcaac agtctactgc tgtggtgact gtgattgtcc 1921 agcctgaaaa caatagacct ccagtggctg tggccggccc tgataaagag ctgatcttcc 1981 cagtggaaag tgctaccctg gatgggagca gcagcagcga tgaccacggc attgtcttct 2041 accactggga gcacgtcaga ggccccagtg cagtggagat ggaaaatatt gacaaagcaa 2101 tagccactgt gactggtctc caggtgggga cctaccactt ccgtttgaca gtgaaagacc 2161 agcagggact gagcagcacg tccaccctca ctgtggctgt gaagaaggaa aataatagtc 2221 ctcccagagc ccgggctggt ggcagacatg ttcttgtgct tcccaataat tccattactt 2281 tggatggttc aaggtctact gatgaccaaa gaattgtgtc ctatctgtgg atccgggatg 2341 gccagagtcc agcagctgga gatgtcatcg atggctctga ccacagtgtg gctctgcagc 2401 ttacgaatct ggtggagggg gtgtacactt tccacttgcg agtcaccgac agtcaggggg 2461 cctcggacac agacactgcc actgtggaag tgcagccaga ccctaggaag agtggcctgg 2521 tggagctgac cctgcaggtt ggtgttgggc agctgacaga gcagcggaag gacacccttg 2581 tgaggcagct ggctgtgctg ctgaacgtgc tggactcgga cattaaggtc cagaagattc 2641 gggcccactc ggatctcagc accgtgattg tgttttatgt acagaacagg ccgcctttca 2701 aggttctcaa agctgctgaa gtggcccgaa atctgcacat gcggctctca aaggagaagg 2761 ctgacttctt gcttttcaag gtcttgaggg ttgatacagc aggttgcctt ctgaagtgtt 2821 ctggccatgg tcactgcgac cccctcacaa agcgctgcat ttgctctcac ttatggatgg 2881 agaaccttat acagcgttat atctgggatg gagagagcaa ctgtgagtgg agtatattct 2941 atgtgacagt gttggctttt actcttattg tgctaacagg aggtttcact tggctttgca 3001 tctgcTGCTG CAAAAGACAA AAAAGGACTA AAATCAGGAA AAAAACAAAG TACACCATCC 3061 TGGATAACAT GGATGAACAG GAAAGAATGG AACTGAGGCC CAAATATGGT ATCAAGCACC 3121 GAAGCACAGA GCACAACTCC AGCCTGATGG TATCCGAGTC TGAGTTTGAC AGTGACCAGG 3181 ACACAATCTT CAGCCGAGAA AAGATGGAGA GAGGGAATCC AAAGGTTTCC ATGAATGGTT 3241 CCATCAGAAA TGGAGCTTCC TTCAGTTATT GCTCAAAGGA CAGATTGCCA ACTTTCTTGT 3301 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3361 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3421 GAAAGGACGA CCGCGCCCGG TTAGCCATAT AGTTACGCGT TAAGTCgaca atcaacctct 3481 ggattacaaa atttgtgaaa gatt