Construct: ORF TRCN0000476470
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014729.1_s317c1
- Derived from:
- ccsbBroadEn_13563
- DNA Barcode:
- GCATGGGTTGGCCGAGGACACCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCARA5 (286133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476470
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286133 | SCARA5 | scavenger receptor class A ... | NM_173833.6 | 79.5% | 78% | (many diffs) |
| 2 | mouse | 71145 | Scara5 | scavenger receptor class A,... | NM_001168318.1 | 83.7% | 84.1% | (many diffs) |
| 3 | mouse | 71145 | Scara5 | scavenger receptor class A,... | NM_028903.2 | 69.8% | 70.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1266
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaacaaagct atgtacctac acaccgtcag cgactgtgac accagctcca 121 tctgtgagga ttcctttgat ggcaggagcc tgtccaagct gaacctgtgt gaggatggtc 181 catgtcacaa acggcgggca agcatctgct gtacccagct ggggtccctg tcggccctga 241 agcatgctgt cctggggctc tacctgctgg tcttcctgat tcttgtgggc atcttcatct 301 tagcagtgtc caggccgcgc agctcccctg acgacctgaa ggccctgact cgcaatgtga 361 accggctgaa tgagagcttc cgggacttgc agctgcggct gctgcaggct ccgctgcaag 421 cggacctgac ggagcaggtg tggaaggtgc aggacgcgct gcagaaccag tcagactcgt 481 tgctggcgct ggcgggcgca gtgcagcggc tggagggcgc gctatggggg ctgcaggcgc 541 aggcggtgca gaccgagcag gcggtggccc tgctgcggga ccgcacgggc cagcagagcg 601 acacggcgca gctggagctc taccagctgc aggtggagag caacagtagc cagctgctgc 661 tgaggcgcca cgcgggcctg ctggacgggc tggcgcgcag ggtgggcatc ctgggcgagg 721 agctggccga cgtgggcggc gtgctgcgcg gcctcaacca cagcctgtcc tacgacgtgg 781 ccctccaccg cacgcggctg caggacctgc gggtgctggt gagcaacgcc agcgaggaca 841 cgcgccgcct gcgcctggcg cacgtaggca tggagctgca gctgaagcag gagctggcca 901 tgctcaacgc ggtcaccgag gacctgcgcc tcaaggactg ggagcactcc atcgcactgc 961 ggaacaTCTC CCTCGCGAAA GGGCCACCGG GACCCAAAGG TGATCAGGGG GATGAAGGAA 1021 AGGAAGGCAG GCCTGGCATC CCTGGATTGC CTGGACTTCG AGGTCTGCCC GGGGAGAGAG 1081 GTACCCCAGG ATTGCCCGGG CCCAAGGGCG ATGATGGGAA GCTGGGGGCC ACAGGACCAA 1141 TGGGCATGCG TGGGTTCAAA GGTGACCGAG GCCCAAAAGG AGAGAAAGGA GAGAAAGGAG 1201 ACAGAGCTGG GGATGCCAGT AAGGACATTC TGCTGGGGCC GTGGGATATG GTGTTGGCAC 1261 AGGGCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCATGGGTT GGCCGAGGAC ACCACACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt