Construct: ORF TRCN0000476514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005274.1_s317c1
- Derived from:
- ccsbBroadEn_00510
- DNA Barcode:
- AATTCTGTCTGTGTGGCATGGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHX2 (2053)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476514
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2053 | EPHX2 | epoxide hydrolase 2 | NM_001979.6 | 100% | 100% | |
| 2 | human | 2053 | EPHX2 | epoxide hydrolase 2 | XM_017013199.1 | 92.6% | 92.6% | 535_536ins123 |
| 3 | human | 2053 | EPHX2 | epoxide hydrolase 2 | NM_001256482.2 | 90.4% | 90.4% | 0_1ins159 |
| 4 | human | 2053 | EPHX2 | epoxide hydrolase 2 | NM_001256484.2 | 90.4% | 90.4% | 0_1ins159 |
| 5 | human | 2053 | EPHX2 | epoxide hydrolase 2 | NM_001256483.2 | 88.1% | 88.1% | 0_1ins198 |
| 6 | human | 2053 | EPHX2 | epoxide hydrolase 2 | XR_001745491.1 | 67.7% | (many diffs) | |
| 7 | human | 2053 | EPHX2 | epoxide hydrolase 2 | XM_017013200.1 | 49.4% | 45.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1731
- ORF length:
- 1665
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gctgcgcgcg gccgtcttcg accttgacgg ggtgctggcg ctgccagcgg 121 tgttcggcgt cctcggccgc acggaggagg ccctggcgct gcccagagga cttctgaatg 181 atgctttcca gaaaggggga ccagagggtg ccactacccg gcttatgaaa ggagagatca 241 cactttccca gtggatacca ctcatggaag aaaactgcag gaagtgctcc gagaccgcta 301 aagtctgcct ccccaagaat ttctccataa aagaaatctt tgacaaggcg atttcagcca 361 gaaagatcaa ccgccccatg ctccaggcag ctctcatgct caggaagaaa ggattcacta 421 ctgccatcct caccaacacc tggctggacg accgtgctga gagagatggc ctggcccagc 481 tgatgtgtga gctgaagatg cactttgact tcctgataga gtcgtgtcag gtgggaatgg 541 tcaaacctga acctcagatc tacaagtttc tgctggacac cctgaaggcc agccccagtg 601 aggtcgtttt tttggatgac atcggggcta atctgaagcc agcccgtgac ttgggaatgg 661 tcaccatcct ggtccaggac actgacacgg ccctgaaaga actggagaaa gtgaccggaa 721 tccagcttct caataccccg gcccctctgc cgacctcttg caatccaagt gacatgagcc 781 atgggtacgt gacagtaaag cccagggtcc gtctgcattt tgtggagctg ggctccggcc 841 ctgctgtgtg cctctgccat ggatttcccg agagttggta ttcttggagg taccagatcc 901 ctgctctggc ccaggcaggt taccgggtcc tagctatgga catgaaaggc tatggagagt 961 catctgctcc tcccgaaata gaagaatatt gcatggaagt gttatgtaag gagatggtaa 1021 ccttcctgga taaactgggc ctctctcaag cagtgttcat tggccatgac tggggtggca 1081 tgctggtgtg gtacatggct ctcttctacc ccgagagagt gagggcggtg gccagtttga 1141 atactccctt cataccagca aatcccaaca tgtccccttt ggagagtatc aaagccaacc 1201 cagtatttga ttaccagctc tacttccaag aaccaggagt ggctgaggct gaactggaac 1261 agaacctgag tcggactttc aaaagcctct tcagagcaag cgatgagagt gttttatcca 1321 tgcataaagt ctgtgaagcg ggaggacttt ttgtaaatag cccagaagag cccagccTCA 1381 GCAGGATGGT CACTGAGGAG GAAATCCAGT TCTATGTGCA GCAGTTCAAG AAGTCTGGTT 1441 TCAGAGGTCC TCTAAACTGG TACCGAAACA TGGAAAGGAA CTGGAAGTGG GCTTGCAAAA 1501 GCTTGGGACG GAAGATCCTG ATTCCGGCCC TGATGGTCAC GGCGGAGAAG GACTTCGTGC 1561 TCGTTCCTCA GATGTCCCAG CACATGGAGG ACTGGATTCC CCACCTGAAA AGGGGACACA 1621 TTGAGGACTG TGGGCACTGG ACACAGATGG ACAAGCCAAC CGAGGTGAAT CAGATCCTCA 1681 TTAAGTGGCT GGATTCTGAT GCCCGGAACC CACCGGTGGT CTCAAAGATG TACCCAACTT 1741 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1801 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1861 CTTGTGGAAA GGACGAAATT CTGTCTGTGT GGCATGGTGT ACGCGTTAAG TCgacaatca 1921 acctctggat tacaaaattt gtgaaagatt