Construct: ORF TRCN0000476524
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006104.1_s317c1
- Derived from:
- ccsbBroadEn_01937
- DNA Barcode:
- GAATCGGAGGCCGCTCTTATATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA7A (8482)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476524
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8482 | SEMA7A | semaphorin 7A (John Milton ... | NM_003612.5 | 100% | 100% | |
| 2 | human | 8482 | SEMA7A | semaphorin 7A (John Milton ... | NM_001146029.3 | 97.8% | 97.8% | 329_330ins42 |
| 3 | human | 8482 | SEMA7A | semaphorin 7A (John Milton ... | NM_001146030.2 | 75.2% | 75.2% | 0_1ins495 |
| 4 | mouse | 20361 | Sema7a | sema domain, immunoglobulin... | NM_011352.2 | 87.5% | 89% | (many diffs) |
| 5 | mouse | 20361 | Sema7a | sema domain, immunoglobulin... | XM_006510888.3 | 66.6% | 69% | (many diffs) |
| 6 | mouse | 20361 | Sema7a | sema domain, immunoglobulin... | XM_006510889.2 | 66.6% | 69% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2067
- ORF length:
- 1998
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacgcctcct ccgcccggac gtgccgcccc cagcgcaccg cgcgcccgcg 121 tccctggccc gccggctcgg ttggggcttc cgctgcggct gcggctgctg ctgctgctct 181 gggcggccgc cgcctccgcc cagggccacc taaggagcgg accccgcatc ttcgccgtct 241 ggaaaggcca tgtagggcag gaccgggtgg actttggcca gactgagccg cacacggtgc 301 ttttccacga gccaggcagc tcctctgtgt gggtgggagg acgtggcaag gtctacctct 361 ttgacttccc cgagggcaag aacgcatctg tgcgcacggt gaatatcggc tccacaaagg 421 ggtcctgtct ggataagcgg gactgcgaga actacatcac tctcctggag aggcggagtg 481 aggggctgct ggcctgtggc accaacgccc ggcaccccag ctgctggaac ctggtgaatg 541 gcactgtggt gccacttggc gagatgagag gctacgcccc cttcagcccg gacgagaact 601 ccctggttct gtttgaaggg gacgaggtgt attccaccat ccggaagcag gaatacaatg 661 ggaagatccc tcggttccgc cgcatccggg gcgagagtga gctgtacacc agtgatactg 721 tcatgcagaa cccacagttc atcaaagcca ccatcgtgca ccaagaccag gcttacgatg 781 acaagatcta ctacttcttc cgagaggaca atcctgacaa gaatcctgag gctcctctca 841 atgtgtcccg tgtggcccag ttgtgcaggg gggaccaggg tggggaaagt tcactgtcag 901 tctccaagtg gaacactttt ctgaaagcca tgctggtatg cagtgatgct gccaccaaca 961 agaacttcaa caggctgcaa gacgtcttcc tgctccctga ccccagcggc cagtggaggg 1021 acaccagggt ctatggtgtt ttctccaacc cctggaacta ctcagccgtc tgtgtgtatt 1081 ccctcggtga cattgacaag gtcttccgta cctcctcact caagggctac cactcaagcc 1141 ttcccaaccc gcggcctggc aagtgcctcc cagaccagca gccgataccc acagagacct 1201 tccaggtggc tgaccgtcac ccagaggtgg cgcagagggt ggagcccatg gggcctctga 1261 agacgccatt gttccactct aaataccact accagaaagt ggccgtccac cgcatgcaag 1321 ccagccacgg ggagaccttt catgtgcttt acctaactac agacaggggc actatccaca 1381 aggtggtgga accgggggag caggagcaca gcttcgcctt caacatcatg gagatccagc 1441 ccttccgccg cgcggctgcc atccagacca tgtcgctgga tgctgagcgg aggaagctgt 1501 atgtgagctc ccagtgggag gtgagccagg tgcccctgga cctgtgtgag gtctatggcg 1561 ggggctgcca cggttgcctc atgtcccgag acccctactg cggctgggac caaggccgct 1621 gcatctccat ctacagctcc gaacggtcag tgctgcaatc cattaatcca gccgagccac 1681 acaaggagtg tcccaacccc aaaccagaca aggccccact gcagaaggtt tccctggccc 1741 caaactctcg ctactaccTG AGCTGCCCCA TGGAATCCCG CCACGCCACC TACTCATGGC 1801 GCCACAAGGA GAACGTGGAG CAGAGCTGCG AACCTGGTCA CCAGAGCCCC AACTGCATCC 1861 TGTTCATCGA GAACCTCACG GCGCAGCAGT ACGGCCACTA CTTCTGCGAG GCCCAGGAGG 1921 GCTCCTACTT CCGCGAGGCT CAGCACTGGC AGCTGCTGCC CGAGGACGGC ATCATGGCCG 1981 AGCACCTGCT GGGTCATGCC TGTGCCCTGG CCGCCTCCCT CTGGCTGGGG GTGCTGCCCA 2041 CACTCACTCT TGGCTTGCTG GTCCACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2101 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2161 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAATCGGA 2221 GGCCGCTCTT ATATCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2281 aagatt