Construct: ORF TRCN0000476531
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016998.1_s317c1
- Derived from:
- ccsbBroadEn_13592
- DNA Barcode:
- GAGGTCTCCCAACTGTGCGTGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GADL1 (339896)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476531
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 339896 | GADL1 | glutamate decarboxylase like 1 | XM_017006298.1 | 81.6% | 81.6% | 0_1ins186 |
| 2 | human | 339896 | GADL1 | glutamate decarboxylase like 1 | XM_017006297.1 | 67.1% | 67.1% | 1_495del |
| 3 | human | 339896 | GADL1 | glutamate decarboxylase like 1 | NM_207359.2 | 64.6% | 64.6% | 1_552del |
| 4 | mouse | 73748 | Gadl1 | glutamate decarboxylase-like 1 | NM_028638.1 | 58.7% | 61.9% | (many diffs) |
| 5 | mouse | 73748 | Gadl1 | glutamate decarboxylase-like 1 | XM_006512319.3 | 56.9% | 60% | (many diffs) |
| 6 | mouse | 73748 | Gadl1 | glutamate decarboxylase-like 1 | XM_017313627.1 | 50.7% | 53.2% | (many diffs) |
| 7 | mouse | 73748 | Gadl1 | glutamate decarboxylase-like 1 | XM_017313628.1 | 30% | 29.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1080
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtatgcaatg aatttagcta gatacaaata ttgtcctgat attaaggaaa 121 aggggctgtc tggttcgcca agattaatcc ttttcacatc tgcagagtgt cattactcta 181 tgaagaaggc agcctctttt cttgggattg gcactgagaa tgtttgcttt gtggaaacag 241 atggaagagg taaaatgata cctgaggaac tggagaagca agtctggcaa gccagaaaag 301 agggggcagc accgtttctt gtctgtgcca cttctggtac aactgtgttg ggagcttttg 361 accctctgga tgaaatagca gacatctgcg agaggcacag cctctggctt catgtagatg 421 cttcttgggg tggctcagct ttgatgtcga ggaagcaccg caagcttctg catggcatcc 481 acagggctga ctctgtggcc tggaacccac acaagatgct gatggctggg atccagtgct 541 gtgctctcct tgtgaaagac aaatctgatc ttcttaaaaa atgctactct gccaaggcat 601 cttacctctt ccagcaggat aaattctatg atgtgagcta tgacacagga gacaagtcta 661 tccagtgtag cagaagacca gatgcattca agttctggat gacctggaag gccctgggta 721 cattaggcct tGAAGAAAGA GTTAATCGTG CTCTTGCTTT ATCTAGGTAC CTAGTAGATG 781 AAATCAAGAA AAGAGAAGGA TTCAAGTTAC TGATGGAACC TGAATATGCC AATATTTGCT 841 TTTGGTACAT TCCACCGAGC CTCAGAGAGA TGGAAGAAGG ACCCGAGTTC TGGGCAAAAC 901 TTAATTTGGT GGCCCCAGCC ATTAAGGAGA GGATGATGAA GAAGGGAAGC TTGATGCTGG 961 GCTACCAGCC GCACCGGGGA AAGGTCAACT TCTTCCGCCA GGTGGTGATC AGCCCTCAAG 1021 TGAGCCGGGA GGACATGGAC TTCCTCCTGG ATGAGATAGA CTTACTGGGT AAAGACATGT 1081 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1141 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1201 TTTATATATC TTGTGGAAAG GACGAGAGGT CTCCCAACTG TGCGTGCGTA CGCGTTAAGT 1261 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt