Construct: ORF TRCN0000476578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001547.1_s317c1
- Derived from:
- ccsbBroadEn_15205
- DNA Barcode:
- TCTGGCCTCCTCATATACATCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAL3ST3 (89792)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89792 | GAL3ST3 | galactose-3-O-sulfotransfer... | NM_033036.3 | 99.8% | 100% | 525A>G;1065G>A |
| 2 | human | 89792 | GAL3ST3 | galactose-3-O-sulfotransfer... | XM_017018519.1 | 91% | 85% | (many diffs) |
| 3 | mouse | 545276 | Gal3st3 | galactose-3-O-sulfotransfer... | NM_001024717.2 | 88.1% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1362
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccacccatc ctccagcgcc tgcagcaggc caccaagatg atgagccgcc 121 ggaaaatcct gctgctggtg ctagggtgca gcaccgtaag ccttctcatc caccaggggg 181 cgcagctcag ctggtacccc aagctgttcc ccttgagctg ccctcctctg cggaactcgc 241 cgccgcgccc caagcacatg actgtggcct tcctgaagac tcacaagacg gcaggcacga 301 cggtgcagaa catcctgttt cgctttgccg agcgccacaa cctgacggtg gccctgccgc 361 acccgagctg cgagcaccag ttctgctacc cccgcaactt ctcggcgcac ttcgtgcacc 421 cggccacgcg gccgccgcac gtgctggcca gccacctgcg cttcgaccgt gcggagctgg 481 agcgcctcat gccgcccagc accgtctatg tcaccatcct gcgcgagccg gccgccatgt 541 tcgagtcgct cttcagctac tacaaccagt actgcccggc cttccggcgc gtgcccaacg 601 cctcgctcga ggccttcctg cgcgcgcccg aggcatacta ccgcgctggc gagcacttcg 661 ccatgttcgc acacaacacg ctggcctacg acctgggcgg cgacaatgag cgcagcccgc 721 gcgacgacgc cgcctacctg gcgggcctca tccgccaggt ggaggaggtt ttctcgctcg 781 tcatgatcgc cgagtacttc gacgagtcgc tagtgctgct gcggcgccta ctggcctggg 841 acctggacga cgtgctctac gccaagctca acgcgcgcgc cgccagctcg cgcctggccg 901 ccatccccgc ggcgctggcg cgggcggcgc gcacctggaa cgccctggac gccggcctct 961 acgaccactt caacgccacc ttctggCGCC ACGTGGCGCG CGCGGGCCGC GCGTGCGTGG 1021 AGCGCGAGGC GCGCGAGCTG CGCGAGGCCC GCCAGCGCCT ACTGCGGCGC TGCTTCGGGG 1081 ACGAGCCACT GCTGCGGCCT GCCGCGCAGA TCCGCACCAA GCAGCTGCAG CCATGGCAGC 1141 CCAGCCGCAA GGTGGACATT ATGGGCTATG ACCTGCCCGG CGGCGGCGCC GGCCCGGCCA 1201 CCGAGGCCTG CCTCAAGCTG GCCATGCCCG AGGTCCAGTA CTCGAACTAC CTGTTGCGCA 1261 AGCAGAAGCG CCGGGGCGGT GCGCGGGCTC GGCCCGAGCC CGTCCTGGAC AATCCCCCGC 1321 CTCGGCCCAT CCGAGTGCTG CCTCGCGGCC CTCAAGGTCC CTTGCCAACT TTCTTGTACA 1381 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1441 AATGAACTAG CCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1501 AGGACGATCT GGCCTCCTCA TATACATCAG TACGCGTTAA GTCgacaatc aacctctgga 1561 ttacaaaatt tgtgaaagat t