Construct: ORF TRCN0000476589
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003576.1_s317c1
- Derived from:
- ccsbBroadEn_08467
- DNA Barcode:
- ATCGAACACGTTAAGGCTATAGCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC38A4 (55089)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476589
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55089 | SLC38A4 | solute carrier family 38 me... | NM_001143824.2 | 99.9% | 100% | 201T>C |
| 2 | human | 55089 | SLC38A4 | solute carrier family 38 me... | NM_018018.5 | 99.9% | 100% | 201T>C |
| 3 | human | 55089 | SLC38A4 | solute carrier family 38 me... | XM_005268997.2 | 99.9% | 100% | 201T>C |
| 4 | mouse | 69354 | Slc38a4 | solute carrier family 38, m... | NM_027052.3 | 82.6% | 87% | (many diffs) |
| 5 | mouse | 69354 | Slc38a4 | solute carrier family 38, m... | XM_006521346.3 | 82.6% | 87% | (many diffs) |
| 6 | mouse | 69354 | Slc38a4 | solute carrier family 38, m... | XM_006521348.3 | 82.6% | 87% | (many diffs) |
| 7 | mouse | 69354 | Slc38a4 | solute carrier family 38, m... | XM_006521349.3 | 82.6% | 87% | (many diffs) |
| 8 | mouse | 69354 | Slc38a4 | solute carrier family 38, m... | XM_006521350.3 | 82.6% | 87% | (many diffs) |
| 9 | mouse | 69354 | Slc38a4 | solute carrier family 38, m... | XM_006521351.2 | 82.6% | 87% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1707
- ORF length:
- 1641
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcccatggaa ctgagaaatg tcaacatcga accagatgat gagagcagca 121 gtggagaaag tgctccagat agctacatcg ggataggaaa ttcagaaaag gcagcaatga 181 gcagtcaatt tgctaatgaa gacactgaaa gtcagaaatt cctgacaaat ggatttttgg 241 ggaaaaagaa gctggcagat tatgccgatg aacaccatcc cggaaccact tcctttggaa 301 tgtcttcatt taacctgagt aatgccatca tgggcagtgg gatcctgggc ttgtcctatg 361 ccatggccaa cacagggatc atacttttta taatcatgct gcttgctgtg gcaatattat 421 cactgtattc agttcacctt ttattaaaaa cagccaagga aggagggtct ttgatttatg 481 aaaaattagg agaaaaggca tttggatggc cgggaaaaat tggagctttt gtttccatta 541 caatgcagaa cattggagca atgtcaagct acctctttat cattaaatat gaactacctg 601 aagtaatcag agcattcatg ggacttgaag aaaatactgg agaatggtac ctcaatggca 661 actacctcat catatttgtg tctgttggaa ttattcttcc actttcgctc cttaaaaatt 721 taggttatct tggctatacc agtggatttt ctcttacctg catggtgttt tttgttagtg 781 tggtgattta caagaaattc caaataccct gccctctacc tgttttggat cacagtgttg 841 gaaatctgtc attcaacaac acgcttccaa tgcatgtggt aatgttaccc aacaactctg 901 agagttctga tgtgaacttc atgatggatt acacccaccg caatcctgca gggctggatg 961 agaaccaggc caagggctct cttcatgaca gtggagtaga atatgaagct catagtgatg 1021 acaagtgtga acccaaatac tttgtattca actcccggac ggcctatgca attcctatcc 1081 tagtatttgc ttttgtatgc caccctgagg tccttcccat ctacagtgaa cttaaagatc 1141 ggtcccggag aaaaatgcaa acggtgtcaa atatttccat cacggggatg cttgtcatgt 1201 acctgcttgc cgccctcttt ggttacctaa ccttctatgg agaagttgaa gatgaattac 1261 ttcatgccta cagcaaagtg tatacattag acatcccTCT TCTCATGGTT CGCCTGGCAG 1321 TCCTTGTGGC AGTAACACTA ACTGTGCCCA TTGTCCTCTT CCCAATTCGT ACATCAGTGA 1381 TCACACTGTT ATTTCCCAAA CGACCCTTCA GCTGGATACG ACATTTCCTG ATTGCAGCTG 1441 TGCTTATTGC ACTTAATAAT GTTCTGGTCA TCCTTGTGCC AACTATAAAA TACATCTTCG 1501 GATTCATAGG GGCTTCTTCT GCCACTATGC TGATTTTTAT TCTTCCAGCA GTTTTTTATC 1561 TTAAACTTGT CAAGAAAGAA ACTTTTAGGT CACCCCAAAA GGTCGGGGCT TTAATTTTCC 1621 TTGTGGTTGG AATATTCTTC ATGATTGGAA GCATGGCACT CATTATAATT GACTGGATTT 1681 ATGATCCTCC AAATTCCAAG CATCACTCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 1741 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 1801 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCGAACAC 1861 GTTAAGGCTA TAGCCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 1921 agatt