Construct: ORF TRCN0000476605

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000701.1_s317c1
Derived from:
ccsbBroadEn_05079
DNA Barcode:
TACTTTCGTGGAAGTTTTCAATTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF217 (154214)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476605
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 154214 RNF217 ring finger protein 217 NM_152553.5 100% 100%
2 human 154214 RNF217 ring finger protein 217 XM_011535500.2 97.8% 97.5% 4_21del
3 human 154214 RNF217 ring finger protein 217 XM_011535494.2 48.4% 48.1% 1_871delinsA;875_880delGCGCCC
4 human 154214 RNF217 ring finger protein 217 XM_011535496.2 44.6% 38.7% (many diffs)
5 human 154214 RNF217 ring finger protein 217 NM_001286398.2 40.7% 40.2% (many diffs)
6 human 154214 RNF217 ring finger protein 217 XR_001743178.1 32.1% (many diffs)
7 human 154214 RNF217 ring finger protein 217 NR_104440.3 7.4% (many diffs)
8 human 154214 RNF217 ring finger protein 217 NR_136734.1 6.9% (many diffs)
9 human 154214 RNF217 ring finger protein 217 XR_942305.3 6.9% (many diffs)
10 human 154214 RNF217 ring finger protein 217 XR_001743179.1 6.8% (many diffs)
11 mouse 268291 Rnf217 ring finger protein 217 NM_001146349.1 39.4% 41.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
891
ORF length:
825
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ggtacaactt ggccaagtag aaatcaaatg ccccatcaca gagtgttttg
121aattcttgga agaaacaact gttgtctata acttaacgca tgaagactcc atcaagtata
181agtacttctt ggaacttggc cgtattgatt ccagcaccaa gccatgtcct cagtgcaagc
241actttacaac cttcaagaaa aaaggacata ttcccacccc ttccagatca gaaagcaaat
301acaaaatcca gtgccctacc tgccaattcg tctggtgttt taagtgccac tctccttggc
361atgaaggtgt taactgcaag gagtacaaaa aaggagacaa attgttgcgt cactgggcca
421gcgaaattga gcatgggcag aggaatgccc agaagtgtcc aaagtgcaag atccacatcc
481agcgaactga aggatgtgac catatgacct gctcacaatg taacactaat ttttgttacc
541gatgtggtga gagataccgc cagcTCCGAT TTTTTGGAGA CCACACATCA AACCTCAGTA
601TATTTGGATG CAAATATCGC TACCTCCCAG AGAGACCTCA TTTAAGGAGA TTAGTGCGAG
661GGTCAGTCTG TGCTGGAAAA TTATTCATTG CACCTCTAAT TATGGTTTTG GGATTGGCAC
721TAGGGGCCAT AGCGGTTGTA ATCGTTGAGG AAATTAAGAC TTACTGGAAC CTCATAAGTG
781GTAGAACCAG GAATCAAACC CAACATTTGG CTCCACAGCC TGTGTTGTTA AGTGATATGC
841TATACTGCCT GAAACAAGTG TTTATTTGTA TTTCCTATCT ATTGCCTTTG TACCCAACTT
901TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
961CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1021CTTGTGGAAA GGACGATACT TTCGTGGAAG TTTTCAATTG ACGCGTTAAG TCgacaatca
1081acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)