Construct: ORF TRCN0000476605
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000701.1_s317c1
- Derived from:
- ccsbBroadEn_05079
- DNA Barcode:
- TACTTTCGTGGAAGTTTTCAATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF217 (154214)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476605
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154214 | RNF217 | ring finger protein 217 | NM_152553.5 | 100% | 100% | |
| 2 | human | 154214 | RNF217 | ring finger protein 217 | XM_011535500.2 | 97.8% | 97.5% | 4_21del |
| 3 | human | 154214 | RNF217 | ring finger protein 217 | XM_011535494.2 | 48.4% | 48.1% | 1_871delinsA;875_880delGCGCCC |
| 4 | human | 154214 | RNF217 | ring finger protein 217 | XM_011535496.2 | 44.6% | 38.7% | (many diffs) |
| 5 | human | 154214 | RNF217 | ring finger protein 217 | NM_001286398.2 | 40.7% | 40.2% | (many diffs) |
| 6 | human | 154214 | RNF217 | ring finger protein 217 | XR_001743178.1 | 32.1% | (many diffs) | |
| 7 | human | 154214 | RNF217 | ring finger protein 217 | NR_104440.3 | 7.4% | (many diffs) | |
| 8 | human | 154214 | RNF217 | ring finger protein 217 | NR_136734.1 | 6.9% | (many diffs) | |
| 9 | human | 154214 | RNF217 | ring finger protein 217 | XR_942305.3 | 6.9% | (many diffs) | |
| 10 | human | 154214 | RNF217 | ring finger protein 217 | XR_001743179.1 | 6.8% | (many diffs) | |
| 11 | mouse | 268291 | Rnf217 | ring finger protein 217 | NM_001146349.1 | 39.4% | 41.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 891
- ORF length:
- 825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggtacaactt ggccaagtag aaatcaaatg ccccatcaca gagtgttttg 121 aattcttgga agaaacaact gttgtctata acttaacgca tgaagactcc atcaagtata 181 agtacttctt ggaacttggc cgtattgatt ccagcaccaa gccatgtcct cagtgcaagc 241 actttacaac cttcaagaaa aaaggacata ttcccacccc ttccagatca gaaagcaaat 301 acaaaatcca gtgccctacc tgccaattcg tctggtgttt taagtgccac tctccttggc 361 atgaaggtgt taactgcaag gagtacaaaa aaggagacaa attgttgcgt cactgggcca 421 gcgaaattga gcatgggcag aggaatgccc agaagtgtcc aaagtgcaag atccacatcc 481 agcgaactga aggatgtgac catatgacct gctcacaatg taacactaat ttttgttacc 541 gatgtggtga gagataccgc cagcTCCGAT TTTTTGGAGA CCACACATCA AACCTCAGTA 601 TATTTGGATG CAAATATCGC TACCTCCCAG AGAGACCTCA TTTAAGGAGA TTAGTGCGAG 661 GGTCAGTCTG TGCTGGAAAA TTATTCATTG CACCTCTAAT TATGGTTTTG GGATTGGCAC 721 TAGGGGCCAT AGCGGTTGTA ATCGTTGAGG AAATTAAGAC TTACTGGAAC CTCATAAGTG 781 GTAGAACCAG GAATCAAACC CAACATTTGG CTCCACAGCC TGTGTTGTTA AGTGATATGC 841 TATACTGCCT GAAACAAGTG TTTATTTGTA TTTCCTATCT ATTGCCTTTG TACCCAACTT 901 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 961 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1021 CTTGTGGAAA GGACGATACT TTCGTGGAAG TTTTCAATTG ACGCGTTAAG TCgacaatca 1081 acctctggat tacaaaattt gtgaaagatt