Construct: ORF TRCN0000476623

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018493.1_s317c1
Derived from:
ccsbBroadEn_07512
DNA Barcode:
ACACAACCGCCAGCAGCAAGTTGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PUM3 (9933)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476623
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9933 PUM3 pumilio RNA binding family ... NM_014878.5 99.8% 99.5% 38G>A;866G>C;889G>T
2 mouse 52874 Pum3 pumilio RNA-binding family ... NM_177474.5 85.7% 89.5% (many diffs)
3 mouse 52874 Pum3 pumilio RNA-binding family ... XM_006527183.3 85.7% 89.5% (many diffs)
4 mouse 52874 Pum3 pumilio RNA-binding family ... NR_131940.1 46.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2010
ORF length:
1944
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga agttaaaggg aaaaagcaat tcacaggaaa gaatacaaag acagcacaag
121aaaaaaacag atttcataaa aatagtgatt ctggttcttc aaagacattt ccaacaagga
181aagttgctaa agaaggtgga cctaaagtca catctaggaa ctttgagaaa agtatcacaa
241aacttgggaa aaagggtgta aagcagttca agaataagca gcaaggggac aaatcaccaa
301agaacaaatt ccagccggca aataaattca acaagaagag aaaattccag ccagatggta
361gaagcgatga atcagcagcc aagaagccca aatgggatga cttcaaaaag aagaagaaag
421aactgaagca aagcagacaa ctcagtgata aaaccaacta tgacattgtt gttcgggcaa
481agcagatgtg ggagatttta agaagaaaag actgtgacaa agaaaaaaga gtaaagttaa
541tgagtgattt gcagaagttg attcaaggga aaattaaaac tattgcattt gcacacgatt
601caactcgtgt gatccagtgt tacattcagt atggtaatga agaacagaga aaacaggctt
661ttgaagaatt gcgagatgat ttggttgagt taagtaaagc caaatattcg agaaatattg
721ttaagaaatt tctcatgtat ggaagtaaac cacagattgc agagataatc agaagtttta
781aaggccacgt gaggaagatg ctgcggcatg cggaagcatc agccatcgtg gagtacgcat
841acaatgacaa agccattttg gagcagagga acatgctgac ggaagagctc tatgggaaca
901catttcagct ttacaagtca gcagatcacc caactctgga caaagtgtta gagttacagc
961cagaaaaatt agaacttatt atggatgaaa tgaaacagat tctaactcca atggcccaaa
1021aggaagctgt gattaagcac tcattggtgc ataaagtatt cttggacttt tttacctatg
1081caccccccaa actcagatca gaaatgattg aagccatccg cgaagcggtg gtctacctgg
1141cacacacaca cgatggcgcc agagtggcca tgcactgcct gtggcatggc acgcccaagg
1201acaggaaagt gattgtgaaa acaatgaaga cttatgttga aaaggtggct aatggccaat
1261actcccattt ggttttactg gcggcatttg attgtattga tgatactaag cttgtgaagc
1321agataatcat atcagaaatt atcagttcat tgcctagcat agtaaatgac aaatatggaa
1381ggaaggtcct attgtactta ctaagcccca gagatcctgc acatacagta cgagaaatca
1441ttgaagttct gcaaaaagga gatggaaatg cacacagtaa gaaagataca gaggtccgca
1501gacgggagct cctagaatcc atttctccag ctttgttaag ctacctgcaa gaacacgccc
1561aagaagtggt gctagataag tctgcgtgtg tgttggtgtc tgacattctg ggatctgcca
1621ctGGAGACGT TCAGCCTACC ATGAATGCCA TCGCCAGCTT GGCAGCAACA GGACTGCATC
1681CTGGTGGCAA GGACGGAGAG CTTCACATTG CAGAACATCC TGCAGGACAT CTAGTTCTGA
1741AGTGGTTAAT AGAGCAAGAT AAAAAGATGA AAGAAAATGG GAGAGAAGGT TGTTTTGCAA
1801AAACACTTGT AGAGCATGTT GGTATGAAGA ACCTGAAGTC CTGGGCTAGT GTAAATCGAG
1861GTGCCATTAT TCTTTCTAGC CTCCTCCAGA GTTGTGACCT GGAAGTTGCA AACAAAGTCA
1921AAGCTGCACT GAAAAGCTTG ATTCCTACAT TGGAAAAAAC CAAAAGCACC AGCAAAGGAA
1981TAGAAATTCT ACTTGAAAAA CTGAGCACAC ACCCAACTTT CTTGTACAAA GTGGTTGATA
2041TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
2101CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACACA
2161ACCGCCAGCA GCAAGTTGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2221tgaaagatt

Download FASTA (ORF) (Full)