Construct: ORF TRCN0000476623
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018493.1_s317c1
- Derived from:
- ccsbBroadEn_07512
- DNA Barcode:
- ACACAACCGCCAGCAGCAAGTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PUM3 (9933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476623
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9933 | PUM3 | pumilio RNA binding family ... | NM_014878.5 | 99.8% | 99.5% | 38G>A;866G>C;889G>T |
| 2 | mouse | 52874 | Pum3 | pumilio RNA-binding family ... | NM_177474.5 | 85.7% | 89.5% | (many diffs) |
| 3 | mouse | 52874 | Pum3 | pumilio RNA-binding family ... | XM_006527183.3 | 85.7% | 89.5% | (many diffs) |
| 4 | mouse | 52874 | Pum3 | pumilio RNA-binding family ... | NR_131940.1 | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2010
- ORF length:
- 1944
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agttaaaggg aaaaagcaat tcacaggaaa gaatacaaag acagcacaag 121 aaaaaaacag atttcataaa aatagtgatt ctggttcttc aaagacattt ccaacaagga 181 aagttgctaa agaaggtgga cctaaagtca catctaggaa ctttgagaaa agtatcacaa 241 aacttgggaa aaagggtgta aagcagttca agaataagca gcaaggggac aaatcaccaa 301 agaacaaatt ccagccggca aataaattca acaagaagag aaaattccag ccagatggta 361 gaagcgatga atcagcagcc aagaagccca aatgggatga cttcaaaaag aagaagaaag 421 aactgaagca aagcagacaa ctcagtgata aaaccaacta tgacattgtt gttcgggcaa 481 agcagatgtg ggagatttta agaagaaaag actgtgacaa agaaaaaaga gtaaagttaa 541 tgagtgattt gcagaagttg attcaaggga aaattaaaac tattgcattt gcacacgatt 601 caactcgtgt gatccagtgt tacattcagt atggtaatga agaacagaga aaacaggctt 661 ttgaagaatt gcgagatgat ttggttgagt taagtaaagc caaatattcg agaaatattg 721 ttaagaaatt tctcatgtat ggaagtaaac cacagattgc agagataatc agaagtttta 781 aaggccacgt gaggaagatg ctgcggcatg cggaagcatc agccatcgtg gagtacgcat 841 acaatgacaa agccattttg gagcagagga acatgctgac ggaagagctc tatgggaaca 901 catttcagct ttacaagtca gcagatcacc caactctgga caaagtgtta gagttacagc 961 cagaaaaatt agaacttatt atggatgaaa tgaaacagat tctaactcca atggcccaaa 1021 aggaagctgt gattaagcac tcattggtgc ataaagtatt cttggacttt tttacctatg 1081 caccccccaa actcagatca gaaatgattg aagccatccg cgaagcggtg gtctacctgg 1141 cacacacaca cgatggcgcc agagtggcca tgcactgcct gtggcatggc acgcccaagg 1201 acaggaaagt gattgtgaaa acaatgaaga cttatgttga aaaggtggct aatggccaat 1261 actcccattt ggttttactg gcggcatttg attgtattga tgatactaag cttgtgaagc 1321 agataatcat atcagaaatt atcagttcat tgcctagcat agtaaatgac aaatatggaa 1381 ggaaggtcct attgtactta ctaagcccca gagatcctgc acatacagta cgagaaatca 1441 ttgaagttct gcaaaaagga gatggaaatg cacacagtaa gaaagataca gaggtccgca 1501 gacgggagct cctagaatcc atttctccag ctttgttaag ctacctgcaa gaacacgccc 1561 aagaagtggt gctagataag tctgcgtgtg tgttggtgtc tgacattctg ggatctgcca 1621 ctGGAGACGT TCAGCCTACC ATGAATGCCA TCGCCAGCTT GGCAGCAACA GGACTGCATC 1681 CTGGTGGCAA GGACGGAGAG CTTCACATTG CAGAACATCC TGCAGGACAT CTAGTTCTGA 1741 AGTGGTTAAT AGAGCAAGAT AAAAAGATGA AAGAAAATGG GAGAGAAGGT TGTTTTGCAA 1801 AAACACTTGT AGAGCATGTT GGTATGAAGA ACCTGAAGTC CTGGGCTAGT GTAAATCGAG 1861 GTGCCATTAT TCTTTCTAGC CTCCTCCAGA GTTGTGACCT GGAAGTTGCA AACAAAGTCA 1921 AAGCTGCACT GAAAAGCTTG ATTCCTACAT TGGAAAAAAC CAAAAGCACC AGCAAAGGAA 1981 TAGAAATTCT ACTTGAAAAA CTGAGCACAC ACCCAACTTT CTTGTACAAA GTGGTTGATA 2041 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2101 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACACA 2161 ACCGCCAGCA GCAAGTTGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2221 tgaaagatt