Construct: ORF TRCN0000476628

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015736.1_s317c1
Derived from:
ccsbBroadEn_11779
DNA Barcode:
GCTTGACAAACGTCGGGACCACTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RCHY1 (25898)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476628
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25898 RCHY1 ring finger and CHY zinc fi... NM_001009922.2 67.3% 67.4% 510_756delinsG
2 human 25898 RCHY1 ring finger and CHY zinc fi... NM_015436.3 65% 65.1% 510_783delinsG
3 human 25898 RCHY1 ring finger and CHY zinc fi... NM_001278538.1 56.5% 54.4% 0_1ins22;22_23ins44;444_717delinsG
4 human 25898 RCHY1 ring finger and CHY zinc fi... NM_001278537.1 51.4% 51.5% 90_91ins120;390_636delinsG
5 human 25898 RCHY1 ring finger and CHY zinc fi... NM_001278536.1 49.6% 49.8% 90_91ins120;390_663delinsG
6 human 25898 RCHY1 ring finger and CHY zinc fi... XM_024453984.1 16.9% 17% 0_1ins381;129_375delinsG
7 human 25898 RCHY1 ring finger and CHY zinc fi... NM_001278539.1 16.3% 16.4% 0_1ins381;129_402delinsG
8 human 25898 RCHY1 ring finger and CHY zinc fi... XM_011531838.2 16.3% 16.4% 0_1ins381;129_402delinsG
9 human 25898 RCHY1 ring finger and CHY zinc fi... XM_011531839.2 16.3% 16.4% 0_1ins381;129_402delinsG
10 human 25898 RCHY1 ring finger and CHY zinc fi... NR_103724.1 11.5% 1_144del;654_4393delinsG
11 human 25898 RCHY1 ring finger and CHY zinc fi... NR_103723.1 11.4% 1_144del;654_4429delinsG
12 human 25898 RCHY1 ring finger and CHY zinc fi... NR_037913.1 11.3% 1_144del;654_4475delinsG
13 human 25898 RCHY1 ring finger and CHY zinc fi... NR_037914.1 11.2% 1_144del;466_467insGTAG;650_4471delinsG
14 human 25898 RCHY1 ring finger and CHY zinc fi... NR_103725.1 9.9% (many diffs)
15 mouse 68098 Rchy1 ring finger and CHY zinc fi... NM_026557.4 57.4% 58.2% (many diffs)
16 mouse 68098 Rchy1 ring finger and CHY zinc fi... NM_001271797.1 43.8% 43.6% (many diffs)
17 mouse 68098 Rchy1 ring finger and CHY zinc fi... XM_006535191.3 28.7% 20.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
576
ORF length:
510
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggcgacggcc cgggaagatg gcgccagcgg tcaagagcga ggtcagcggg
121gctgcgagca ctatgacaga ggatgtctcc TAAAGGCACC TTGCTGTGAC AAGCTTTATA
181CTTGCCGCTT GTGTCATGAT AACAATGAAG ATCATCAACT AGATCGCTTT AAAGTGAAGG
241AAGTGCAGTG CATAAACTGT GAAAAAATTC AACATGCCCA ACAGACTTGT GAAGAATGTA
301GCACATTGTT TGGAGAATAT TATTGCGATA TATGCCATTT GTTTGACAAA GATAAGAAGC
361AGTATCACTG TGAAAACTGT GGAATTTGTA GGATTGGTCC AAAGGAAGAT TTTTTCCATT
421GTTTGAAATG TAACTTATGC CTAGCTATGA ATCTTCAAGG AAGACACAAG TGTATTGAAA
481ATGTGTCCCG ACAGAATTGT CCAATATGTT TGGAGGACAT TCACACATCC CGTGTTGTTG
541CTCATGTCTT GCCATGTGGA CATCTTTTAC ATAGGTACCC AACTTTCTTG TACAAAGTGG
601TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
661CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
721AGCTTGACAA ACGTCGGGAC CACTTACGCG TTAAGTCgac aatcaacctc tggattacaa
781aatttgtgaa agatt

Download FASTA (ORF) (Full)