Transcript: Human XM_024453984.1

PREDICTED: Homo sapiens ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RCHY1 (25898)
Length:
4057
CDS:
316..693

Additional Resources:

NCBI RefSeq record:
XM_024453984.1
NBCI Gene record:
RCHY1 (25898)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453984.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000293609 GGAGAATATTATTGCGATATA pLKO_005 181 5UTR 100% 13.200 18.480 N RCHY1 n/a
2 TRCN0000004089 GCTCAATTAGGGCAGTATCAT pLKO.1 1263 3UTR 100% 5.625 7.875 N RCHY1 n/a
3 TRCN0000004088 TGCTTTAGATATGACCAGGTA pLKO.1 474 CDS 100% 2.640 3.696 N RCHY1 n/a
4 TRCN0000293544 TATCATGTGTCGTCATCTATG pLKO_005 762 3UTR 100% 10.800 8.640 N RCHY1 n/a
5 TRCN0000298529 AGGCTACAGATGTCCATTATG pLKO_005 444 CDS 100% 13.200 9.240 N RCHY1 n/a
6 TRCN0000298527 GATTTGTGAATCCTATAATAC pLKO_005 630 CDS 100% 13.200 9.240 N RCHY1 n/a
7 TRCN0000004090 CCCGTGTTGTTGCTCATGTCT pLKO.1 398 CDS 100% 3.000 2.100 N RCHY1 n/a
8 TRCN0000010849 AGTGAAGGAAGTGCAGTGCAT pLKO.1 102 5UTR 100% 2.640 1.848 N RCHY1 n/a
9 TRCN0000004091 GCAATGACTGTAATGGACGAT pLKO.1 572 CDS 100% 2.640 1.848 N RCHY1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453984.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07961 pDONR223 100% 47.7% 47.5% None 0_1ins381;44G>A;128_129ins27 n/a
2 ccsbBroad304_07961 pLX_304 0% 47.7% 47.5% V5 0_1ins381;44G>A;128_129ins27 n/a
3 TRCN0000472166 ATTAGGATACGCCAGGGCAGTGCG pLX_317 50.8% 47.7% 47.5% V5 0_1ins381;44G>A;128_129ins27 n/a
4 ccsbBroadEn_11779 pDONR223 100% 16.9% 17% None 0_1ins381;129_375delinsG n/a
5 ccsbBroad304_11779 pLX_304 0% 16.9% 17% V5 0_1ins381;129_375delinsG n/a
6 TRCN0000476628 GCTTGACAAACGTCGGGACCACTT pLX_317 82.2% 16.9% 17% V5 0_1ins381;129_375delinsG n/a
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