Transcript: Mouse NM_026557.4

Mus musculus ring finger and CHY zinc finger domain containing 1 (Rchy1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Rchy1 (68098)
Length:
2029
CDS:
350..1135

Additional Resources:

NCBI RefSeq record:
NM_026557.4
NBCI Gene record:
Rchy1 (68098)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_026557.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000217325 GGTGATTGTAATAGCGATTAC pLKO.1 1310 3UTR 100% 1.080 1.512 N Rchy1 n/a
2 TRCN0000241092 TAGTCACATTCATCGAAATAT pLKO_005 1411 3UTR 100% 15.000 10.500 N Rchy1 n/a
3 TRCN0000241093 CCGGCAGAATTGTCCAATATG pLKO_005 772 CDS 100% 13.200 9.240 N Rchy1 n/a
4 TRCN0000241090 CTCCTACATAGAACGTGTTAT pLKO_005 848 CDS 100% 13.200 9.240 N Rchy1 n/a
5 TRCN0000241091 ACTCCCATGCCATCCGAATAC pLKO_005 968 CDS 100% 10.800 7.560 N Rchy1 n/a
6 TRCN0000194505 GAATACCAGAACGTGACTGTT pLKO.1 983 CDS 100% 4.950 3.465 N Rchy1 n/a
7 TRCN0000004091 GCAATGACTGTAATGGACGAT pLKO.1 1014 CDS 100% 2.640 1.848 N RCHY1 n/a
8 TRCN0000215631 GAGAATATTACTGCAGTATTT pLKO.1 597 CDS 100% 13.200 7.920 N Rchy1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_026557.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07961 pDONR223 100% 87.8% 90% None (many diffs) n/a
2 ccsbBroad304_07961 pLX_304 0% 87.8% 90% V5 (many diffs) n/a
3 TRCN0000472166 ATTAGGATACGCCAGGGCAGTGCG pLX_317 50.8% 87.8% 90% V5 (many diffs) n/a
4 ccsbBroadEn_11779 pDONR223 100% 57.4% 58.2% None (many diffs) n/a
5 ccsbBroad304_11779 pLX_304 0% 57.4% 58.2% V5 (many diffs) n/a
6 TRCN0000476628 GCTTGACAAACGTCGGGACCACTT pLX_317 82.2% 57.4% 58.2% V5 (many diffs) n/a
Download CSV