Construct: ORF TRCN0000476634

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010289.1_s317c1
Derived from:
ccsbBroadEn_07057
DNA Barcode:
CGTCATACCTCACTCTAATCTTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TGFB2 (7042)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476634
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7042 TGFB2 transforming growth factor ... NM_003238.6 99.9% 99.7% 1241G>T
2 human 7042 TGFB2 transforming growth factor ... NM_001135599.3 93.5% 93.2% 347_430del;1325G>T
3 human 7042 TGFB2 transforming growth factor ... NR_138148.1 18.9% (many diffs)
4 mouse 21808 Tgfb2 transforming growth factor,... NM_009367.4 89.2% 95.6% (many diffs)
5 mouse 21808 Tgfb2 transforming growth factor,... NM_001329107.1 83.5% 89.3% (many diffs)
6 mouse 21808 Tgfb2 transforming growth factor,... XM_006497136.3 66.5% 59% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1311
ORF length:
1242
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gcactactgt gtgctgagcg cttttctgat cctgcatctg gtcacggtcg
121cgctcagcct gtctacctgc agcacactcg atatggacca gttcatgcgc aagaggatcg
181aggcgatccg cgggcagatc ctgagcaagc tgaagctcac cagtccccca gaagactatc
241ctgagcccga ggaagtcccc ccggaggtga tttccatcta caacagcacc agggacttgc
301tccaggagaa ggcgagccgg agggcggccg cctgcgagcg cgagaggagc gacgaagagt
361actacgccaa ggaggtttac aaaatagaca tgccgccctt cttcccctcc gaaaatgcca
421tcccgcccac tttctacaga ccctacttca gaattgttcg atttgacgtc tcagcaatgg
481agaagaatgc ttccaatttg gtgaaagcag agttcagagt ctttcgtttg cagaacccaa
541aagccagagt gcctgaacaa cggattgagc tatatcagat tctcaagtcc aaagatttaa
601catctccaac ccagcgctac atcgacagca aagttgtgaa aacaagagca gaaggcgaat
661ggctctcctt cgatgtaact gatgctgttc atgaatggct tcaccataaa gacaggaacc
721tgggatttaa aataagctta cactgtccct gctgcacttt tgtaccatct aataattaca
781tcatcccaaa taaaagtgaa gaactagaag caagatttgc aggtattgat ggcacctcca
841catataccag tggtgatcag aaaactataa agtccactag gaaaaaaaac agtgggaaga
901ccccacatct cctgctaatg ttattgcccT CCTACAGACT TGAGTCACAA CAGACCAACC
961GGCGGAAGAA GCGTGCTTTG GATGCGGCCT ATTGCTTTAG AAATGTGCAG GATAATTGCT
1021GCCTACGTCC ACTTTACATT GATTTCAAGA GGGATCTAGG GTGGAAATGG ATACACGAAC
1081CCAAAGGGTA CAATGCCAAC TTCTGTGCTG GAGCATGCCC GTATTTATGG AGTTCAGACA
1141CTCAGCACAG CAGGGTCCTG AGCTTATATA ATACCATAAA TCCAGAAGCA TCTGCTTCTC
1201CTTGCTGCGT GTCCCAAGAT TTAGAACCTC TAACCATTCT CTACTACATT GGCAAAACAC
1261CCAAGATTGA ACAGCTTTCT AATATGATTG TAAAGTCTTG CAAATGCATC TTGCCAACTT
1321TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1381CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1441CTTGTGGAAA GGACGACGTC ATACCTCACT CTAATCTTTC ACGCGTTAAG TCgacaatca
1501acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)