Construct: ORF TRCN0000476634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010289.1_s317c1
- Derived from:
- ccsbBroadEn_07057
- DNA Barcode:
- CGTCATACCTCACTCTAATCTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGFB2 (7042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7042 | TGFB2 | transforming growth factor ... | NM_003238.6 | 99.9% | 99.7% | 1241G>T |
| 2 | human | 7042 | TGFB2 | transforming growth factor ... | NM_001135599.3 | 93.5% | 93.2% | 347_430del;1325G>T |
| 3 | human | 7042 | TGFB2 | transforming growth factor ... | NR_138148.1 | 18.9% | (many diffs) | |
| 4 | mouse | 21808 | Tgfb2 | transforming growth factor,... | NM_009367.4 | 89.2% | 95.6% | (many diffs) |
| 5 | mouse | 21808 | Tgfb2 | transforming growth factor,... | NM_001329107.1 | 83.5% | 89.3% | (many diffs) |
| 6 | mouse | 21808 | Tgfb2 | transforming growth factor,... | XM_006497136.3 | 66.5% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1311
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcactactgt gtgctgagcg cttttctgat cctgcatctg gtcacggtcg 121 cgctcagcct gtctacctgc agcacactcg atatggacca gttcatgcgc aagaggatcg 181 aggcgatccg cgggcagatc ctgagcaagc tgaagctcac cagtccccca gaagactatc 241 ctgagcccga ggaagtcccc ccggaggtga tttccatcta caacagcacc agggacttgc 301 tccaggagaa ggcgagccgg agggcggccg cctgcgagcg cgagaggagc gacgaagagt 361 actacgccaa ggaggtttac aaaatagaca tgccgccctt cttcccctcc gaaaatgcca 421 tcccgcccac tttctacaga ccctacttca gaattgttcg atttgacgtc tcagcaatgg 481 agaagaatgc ttccaatttg gtgaaagcag agttcagagt ctttcgtttg cagaacccaa 541 aagccagagt gcctgaacaa cggattgagc tatatcagat tctcaagtcc aaagatttaa 601 catctccaac ccagcgctac atcgacagca aagttgtgaa aacaagagca gaaggcgaat 661 ggctctcctt cgatgtaact gatgctgttc atgaatggct tcaccataaa gacaggaacc 721 tgggatttaa aataagctta cactgtccct gctgcacttt tgtaccatct aataattaca 781 tcatcccaaa taaaagtgaa gaactagaag caagatttgc aggtattgat ggcacctcca 841 catataccag tggtgatcag aaaactataa agtccactag gaaaaaaaac agtgggaaga 901 ccccacatct cctgctaatg ttattgcccT CCTACAGACT TGAGTCACAA CAGACCAACC 961 GGCGGAAGAA GCGTGCTTTG GATGCGGCCT ATTGCTTTAG AAATGTGCAG GATAATTGCT 1021 GCCTACGTCC ACTTTACATT GATTTCAAGA GGGATCTAGG GTGGAAATGG ATACACGAAC 1081 CCAAAGGGTA CAATGCCAAC TTCTGTGCTG GAGCATGCCC GTATTTATGG AGTTCAGACA 1141 CTCAGCACAG CAGGGTCCTG AGCTTATATA ATACCATAAA TCCAGAAGCA TCTGCTTCTC 1201 CTTGCTGCGT GTCCCAAGAT TTAGAACCTC TAACCATTCT CTACTACATT GGCAAAACAC 1261 CCAAGATTGA ACAGCTTTCT AATATGATTG TAAAGTCTTG CAAATGCATC TTGCCAACTT 1321 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1381 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1441 CTTGTGGAAA GGACGACGTC ATACCTCACT CTAATCTTTC ACGCGTTAAG TCgacaatca 1501 acctctggat tacaaaattt gtgaaagatt