Transcript: Mouse NM_009367.4

Mus musculus transforming growth factor, beta 2 (Tgfb2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Tgfb2 (21808)
Length:
5116
CDS:
1216..2460

Additional Resources:

NCBI RefSeq record:
NM_009367.4
NBCI Gene record:
Tgfb2 (21808)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009367.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000066096 CCCTCGACATGGATCAGTTTA pLKO.1 1292 CDS 100% 13.200 18.480 N Tgfb2 n/a
2 TRCN0000313040 TACTACGCCAAGGAGGTTTAT pLKO_005 1507 CDS 100% 13.200 18.480 N Tgfb2 n/a
3 TRCN0000349810 AGGCGAGATTTGCAGGTATTG pLKO_005 1955 CDS 100% 10.800 15.120 N Tgfb2 n/a
4 TRCN0000066094 GCCGTCTAATAATTACATCAT pLKO.1 1911 CDS 100% 4.950 6.930 N Tgfb2 n/a
5 TRCN0000312049 GCCGTCTAATAATTACATCAT pLKO_005 1911 CDS 100% 4.950 6.930 N Tgfb2 n/a
6 TRCN0000066097 CCGCTTTGATGTCTCAACAAT pLKO.1 1605 CDS 100% 5.625 4.500 N Tgfb2 n/a
7 TRCN0000313122 AGCCTGTACAACACCATAAAT pLKO_005 2308 CDS 100% 15.000 10.500 N Tgfb2 n/a
8 TRCN0000066093 CGGTGAAATGTTGACCTGTTT pLKO.1 3311 3UTR 100% 4.950 3.465 N Tgfb2 n/a
9 TRCN0000312105 CGGTGAAATGTTGACCTGTTT pLKO_005 3311 3UTR 100% 4.950 3.465 N Tgfb2 n/a
10 TRCN0000033426 GCTTTAGAAATGTGCAGGATA pLKO.1 2141 CDS 100% 4.950 3.465 N TGFB2 n/a
11 TRCN0000066095 GCTTTCCAATATGATTGTCAA pLKO.1 2421 CDS 100% 4.950 3.465 N Tgfb2 n/a
12 TRCN0000166364 CACACACACACACACACACAA pLKO.1 4311 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009367.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07057 pDONR223 100% 89.2% 95.6% None (many diffs) n/a
2 ccsbBroad304_07057 pLX_304 0% 89.2% 95.6% V5 (many diffs) n/a
3 TRCN0000476634 CGTCATACCTCACTCTAATCTTTC pLX_317 30.9% 89.2% 95.6% V5 (many diffs) n/a
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