Construct: ORF TRCN0000476643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002715.1_s317c1
- Derived from:
- ccsbBroadEn_00692
- DNA Barcode:
- CTTTACAACCGGTCCTGCCTGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRINA (2907)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476643
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2907 | GRINA | glutamate ionotropic recept... | NM_000837.1 | 100% | 100% | |
| 2 | human | 2907 | GRINA | glutamate ionotropic recept... | NM_001009184.2 | 99.9% | 100% | 993A>G |
| 3 | mouse | 66168 | Grina | glutamate receptor, ionotro... | NM_023168.3 | 84.1% | 87.6% | (many diffs) |
| 4 | mouse | 66168 | Grina | glutamate receptor, ionotro... | XM_006521249.3 | 84.1% | 87.6% | (many diffs) |
| 5 | mouse | 66168 | Grina | glutamate receptor, ionotro... | XM_006521250.3 | 84.1% | 87.6% | (many diffs) |
| 6 | mouse | 66168 | Grina | glutamate receptor, ionotro... | XM_006521251.3 | 84.1% | 87.6% | (many diffs) |
| 7 | mouse | 66168 | Grina | glutamate receptor, ionotro... | XM_011245709.2 | 84.1% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1182
- ORF length:
- 1113
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcccatgaa aagagttttt tggtgtctgg ggacaactat cctcccccca 121 accctggata tccggggggg ccccagccac ccatgccccc ctatgctcag cctccctacc 181 ctggggcccc ttacccacag ccccctttcc agccctcccc ctacggtcag ccagggtacc 241 cccatggccc cagcccctac ccccaagggg gctacccaca gggtccctac ccccaagggg 301 gctacccaca gggcccctac ccacaagagg gctacccaca gggcccctac ccccaagggg 361 gctaccccca ggggccatat ccccagagcc ccttcccccc caacccctat ggacagccac 421 aggtcttccc aggacaagac cctgactcac cccagcatgg aaactaccag gaggagggtc 481 ccccatccta ctatgacaac caggacttcc ctgccaccaa ctgggatgac aagagcatcc 541 gacaggcctt catccgcaag gtgttcctag tgctgacctt gcagctgtcg gtgaccctgt 601 ccacggtgtc tgtgttcact tttgttgcgg aggtgaaggg ctttgtccgg gagaatgtct 661 ggacctacta tgtctcctat gctgtcttct tcatctctct catcgtcctc agctgttgtg 721 gggacttccg gcgaaagcac ccctggaacc ttgttgcact gtcggtcctg accgccagcc 781 tgtcgtacat ggtggggatg atcgccagct tctacaacac cgaggcagtc atcatggccg 841 tgggcatcac cacagccgtc tgcttcaccg tcgtcatctt ctccatgcag acccgctacg 901 acttcaccTC ATGCATGGGC GTGCTCCTGG TGAGCATGGT GGTGCTCTTC ATCTTCGCCA 961 TTCTCTGCAT CTTCATCCGG AACCGCATCC TGGAGATCGT GTACGCCTCA CTGGGCGCTC 1021 TGCTCTTCAC CTGCTTCCTC GCAGTGGACA CCCAGCTGCT GCTGGGGAAC AAGCAGCTGT 1081 CCCTGAGCCC AGAAGAGTAT GTGTTTGCTG CGCTGAACCT GTACACAGAC ATCATCAACA 1141 TCTTCCTGTA CATCCTCACC ATCATTGGCC GCGCCAAGGA GTTGCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGACTT TACAACCGGT CCTGCCTGGA CACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t