Construct: ORF TRCN0000476700
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012045.1_s317c1
- Derived from:
- BRDN0000391335
- DNA Barcode:
- TCCTCAAGAACCATGTATGTCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLSTN3 (9746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476700
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9746 | CLSTN3 | calsyntenin 3 | NM_014718.4 | 99.8% | 99.6% | (many diffs) |
| 2 | human | 9746 | CLSTN3 | calsyntenin 3 | XM_006719163.4 | 97.6% | 96.7% | (many diffs) |
| 3 | mouse | 232370 | Clstn3 | calsyntenin 3 | NM_153508.4 | 89.6% | 95.5% | (many diffs) |
| 4 | mouse | 232370 | Clstn3 | calsyntenin 3 | NM_001346656.1 | 86.4% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2937
- ORF length:
- 2868
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccctcctg ctgctggaca ttctgctggc ctctctgctc gcgtcctgct 121 cctgtaacaa agccaacaag cacaagccat ggattgaggc agagtaccag ggcatcgtca 181 tggagaatga caacacggtc ctactgaatc caccactctt tgccttggac aaggatgccc 241 cgctgcgcta tgcaggtgag atctgcggct tccggctcca tgggtctggg gtgccctttg 301 aggctgtgat ccttgacaag gcgacaggag aggggctgat ccgggccaag gagcctgtgg 361 actgcgaggc ccagaaggaa cacaccttca ccatccaggc ctatgactgt ggcgagggcc 421 ccgacggggc caacaccaag aagtcccaca aggccactgt gcatgtgcgg gtcaacgatg 481 tgaacgagtt tgccccagtg tttgtggaac ggctgtatcg tgcggctgtg acagagggga 541 agctgtacga tcgcatcctg cgggtggaag ccattgacgg tgactgctcc ccccagtaca 601 gccagatctg ctactatgag attctcacac ccaacacccc tttcctcatt gacaatgacg 661 ggaacattga gaacacagag aagctgcagt acagtggtga gaggctctat aagtttacag 721 tgacagctta tgactgtggg aagaagcggg cagcagatga tgctgaggtg gagattcagg 781 tgaagcccac ctgtaaaccc agctggcaag gctggaacaa aaggatcgaa tatgcaccag 841 gtgctgggag cttggctttg ttccctggta tccgcctgga gacctgtgat gaaccactct 901 ggaacattca ggccaccata gagctgcaga ccagccatgt ggccaagggc tgtgaccgtg 961 acaactactc agagcgggcg ctgcggaaac tctgtggtgc tgccactggg gaggtggatc 1021 tgttgcccat gcctggcccc aatgccaact ggacagcagg actctcggtg cactacagcc 1081 aggacagcag cctgatctac tggttcaatg gcacccaggc tgtgcaggtg cccctgggtg 1141 gccccagtgg gctgggctct gggccccagg acagcctcag tgaccacttc accctgtcct 1201 tctggatgaa gcatggcgta actcccaaca agggcaagaa ggaagaggaa accatcgtat 1261 gtaacactgt ccagaatgag gacggcttct ctcactactc gctgactgtc cacggctgta 1321 ggattgcctt cctctactgg cccctgcttg agagtgcccg cccagtcaag ttcctctgga 1381 agctggagca ggtctgtgat gatgagtggc accactacgc tctgaacctc gagttcccca 1441 cagtcacact ctataccgac ggcatctcct tcgaccctgc cctcatccat gacaatggcc 1501 tcatccaccc accccgaagg gagcctgctc tcatgattgg ggcctgctgg actgaggaga 1561 agaacaaaga gaaggaaaag ggagacaaca gtacagacac cacccaagga gaccctttgt 1621 cgatccacca ctacttccat ggctacctgg ctggtttcag cgtgcgctca ggtcgcctgg 1681 agagccgcga ggtcatcgag tgcctctatg catgtcggga ggggctggac tatagggatt 1741 tcgagagcct gggcaaaggc atgaaggtcc acgtgaaccc ctcacagtcc ctgctcaccc 1801 tggaggggga tgatgtggag accttcaacc atgccctgca gcatgtggct tacatgaaca 1861 ctctgcgctt tgccacgccc ggcgtcaggc ccctgcgcct caccactgct gtcaagtgct 1921 tcagcgaaga gtcctgcgtc tccatccctg aagtggaggg ctacgtggtc gtccttcagc 1981 ctgacgcccc ccagatcctg ctgagtggca ctgctcattt tgcccgccca gctgtggact 2041 ttgagggaac caacggcgtc cctttgttcc ctgatcttca aatcacctgc tccatttctc 2101 accaggtgga ggccaaaaag gatgagagtt ggcagggcac agtgacagac acacgcatgt 2161 cggatgagat tgtgcacaac ctggatggct gtgaaatttc tctggtgggg gatgacctgg 2221 atcccgagcg ggaaatcctg ctgctggaca caacctctct gcagcagcgg gggctggagc 2281 tcaccaacac atctgcctac ctcactattg ctggggtgga gagcatcact gtgtatgaag 2341 agatcctgag gcaggctcgt tatcggctgc gacacggagc tgccctctac accaggaagt 2401 tccggctttc ctgctcggaa atgaatggcc gttactccag caatgaattc atcgtggagg 2461 tcaatgtcct gcacagcatg aaccgggttg cccaccccag ccacgtgctc agctcccagc 2521 agttcctgca ccgtggtcac cagcccccgc ctgagatggc tggacacagc ctagccagct 2581 cccacagaaa ctccatgata cccagcgccg caacccTCAT CATTGTGGTG TGCGTGGGCT 2641 TCCTGGTGCT CATGGTCGTC CTGGGCCTGG TGCGCATCCA TTCCCTTCAC CGCCGCGTCT 2701 CAGGGGCCGG CGGGCCTCCA GGGGCCTCCA GTGACCCCAA GGACCCAGAC CTCTTCTGGG 2761 ATGACTCAGC TCTCACCATC ATTGTGAACC CCATGGAGTC CTACCAGAAT CGGCAGTCCT 2821 GTGTGACGGG GGCTGTTGGG GGCCAGCAGG AGGATGAGGA CAGCAGTGAC TCGGAGGTGG 2881 CCGATTCCCC CAGCAGCGAC GAGAGACGCA TCATCGAGAC CCCCCCACAC CGCTACTTGC 2941 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3001 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3061 ATATATCTTG TGGAAAGGAC GATCCTCAAG AACCATGTAT GTCATTACGC GTTAAGTCga 3121 caatcaacct ctggattaca aaatttgtga aagatt