Construct: ORF TRCN0000476705
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008308.1_s317c1
- Derived from:
- ccsbBroadEn_05882
- DNA Barcode:
- GTACTGAGTATCCACTCCTTGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCL6 (604)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476705
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 604 | BCL6 | BCL6 transcription repressor | NM_001130845.2 | 99.9% | 99.8% | 478C>T;1161C>T |
| 2 | human | 604 | BCL6 | BCL6 transcription repressor | NM_001706.5 | 99.9% | 99.8% | 478C>T;1161C>T |
| 3 | human | 604 | BCL6 | BCL6 transcription repressor | XM_005247694.4 | 99.9% | 99.8% | 478C>T;1161C>T |
| 4 | human | 604 | BCL6 | BCL6 transcription repressor | NM_001134738.1 | 91.9% | 91.9% | 478C>T;1161C>T;1539_1540ins168 |
| 5 | human | 604 | BCL6 | BCL6 transcription repressor | XM_011513062.3 | 91.9% | 91.9% | 478C>T;1161C>T;1539_1540ins168 |
| 6 | mouse | 12053 | Bcl6 | B cell leukemia/lymphoma 6 | NM_001348026.1 | 89% | 94.4% | (many diffs) |
| 7 | mouse | 12053 | Bcl6 | B cell leukemia/lymphoma 6 | NM_009744.4 | 89% | 94.4% | (many diffs) |
| 8 | mouse | 12053 | Bcl6 | B cell leukemia/lymphoma 6 | XM_017316855.1 | 89% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2187
- ORF length:
- 2118
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctcgccg gctgacagct gtatccagtt cacccgccat gccagtgatg 121 ttcttctcaa ccttaatcgt ctccggagtc gagacatctt gactgatgtt gtcattgttg 181 tgagccgtga gcagtttaga gcccataaaa cggtcctcat ggcctgcagt ggcctgttct 241 atagcatctt tacagaccag ttgaaatgca accttagtgt gatcaatcta gatcctgaga 301 tcaaccctga gggattctgc atcctcctgg acttcatgta cacatctcgg ctcaatttgc 361 gggagggcaa catcatggct gtgatggcca cggctatgta cctgcagatg gagcatgttg 421 tggacacttg ccggaagttt attaaggcca gtgaagcaga gatggtttct gccatcaagc 481 ctcctcgtga agagttcctc aacagccgga tgctgatgcc ccaagacatc atggcctatc 541 ggggttgtga ggtggtggag aacaacctgc cactgaggag cgcccctggg tgtgagagca 601 gagcctttgc ccccagcctg tacagtggcc tgtccacacc gccagcctct tattccatgt 661 acagccacct ccctgtcagc agcctcctct tctccgatga ggagtttcgg gatgtccgga 721 tgcctgtggc caaccccttc cccaaggagc gggcactccc atgtgatagt gccaggccag 781 tccctggtga gtacagccgg ccgactttgg aggtgtcccc caatgtgtgc cacagcaata 841 tctattcacc caaggaaaca atcccagaag aggcacgaag tgatatgcac tacagtgtgg 901 ctgagggcct caaacctgct gccccctcag cccgaaatgc cccctacttc ccttgtgaca 961 aggccagcaa agaagaagag agaccctcct cggaagatga gattgccctg catttcgagc 1021 cccccaatgc acccctgaac cggaagggtc tggttagtcc acagagcccc cagaaatctg 1081 actgccagcc caactcgccc acagagtcct gcagcagtaa gaatgcctgc atcctccagg 1141 cttctggctc ccctccagcc aagagcccca ctgaccccaa agcctgcaac tggaagaaat 1201 acaagttcat cgtgctcaac agcctcaatc agaatgccaa accagagggg cctgagcagg 1261 ctgagctggg ccgcctttcc ccacgagcct acacggcccc acctgcctgc cagccaccca 1321 tggagcctga gaaccttgac ctccagtccc caaccaagct gagtgccagc ggggaggact 1381 ccaccatccc acaagccagc cggctcaata acatcgttaa caggtccatg acgggctctc 1441 cccgcagcag cagcgagagc cactcaccac tctacatgca ccccccgaag tgcacgtcct 1501 gcggctctca gtccccacag catgcagaga tgtgcctcca caccgctggc cccacgttcc 1561 ctgaggagat gggagagacc cagtctgagt actcagattc tagctgtgag aacggggcct 1621 tcttctgcaa tgagtgtgac tgccgcttct ctgaggaggc ctcactcaag aggcacacgc 1681 tgcagaccca cagtgacaaa ccctacaagt gtgaccgctg ccaggcctcc ttccgctaca 1741 agggcaacct cgccagccac aagaccgtcc ataccggtga gaaaccctat cgttgcaaca 1801 tctgtggggc ccagttcaac cggccagcca acctgaaaac ccacactcga attcactctg 1861 gagagaagcc ctacaaatgc gaaacctgcg gagccagatt tgtacaggtg gcccaccTCC 1921 GTGCCCATGT GCTTATCCAC ACTGGTGAGA AGCCCTATCC CTGTGAAATC TGTGGCACCC 1981 GTTTCCGGCA CCTTCAGACT CTGAAGAGCC ACCTGCGAAT CCACACAGGA GAGAAACCTT 2041 ACCATTGTGA GAAGTGTAAC CTGCATTTCC GTCACAAAAG CCAGCTGCGA CTTCACTTGC 2101 GCCAGAAGCA TGGCGCCATC ACCAACACCA AGGTGCAATA CCGCGTGTCA GCCACTGACC 2161 TGCCTCCGGA GCTCCCCAAA GCCTGCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2221 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2281 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTACTGAG 2341 TATCCACTCC TTGTCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2401 aagatt