Construct: ORF TRCN0000476738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011796.1_s317c1
- Derived from:
- ccsbBroadEn_08738
- DNA Barcode:
- AAGCCACTAAGTTGCCGGTGGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTUS1 (57509)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476738
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_020749.4 | 99.9% | 100% | 1245C>T |
| 2 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001166393.1 | 93.1% | 90.8% | (many diffs) |
| 3 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001001931.2 | 80.9% | 76.8% | (many diffs) |
| 4 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001330470.1 | 77.5% | 75% | (many diffs) |
| 5 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001001925.2 | 34.6% | 32.9% | (many diffs) |
| 6 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001363062.1 | 34.6% | 32.9% | (many diffs) |
| 7 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001363060.2 | 34.1% | 27.7% | (many diffs) |
| 8 | human | 57509 | MTUS1 | microtubule associated scaf... | XM_005273581.3 | 34.1% | 27.7% | (many diffs) |
| 9 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001001924.2 | 33.1% | 31.5% | (many diffs) |
| 10 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001363058.1 | 33.1% | 31.5% | (many diffs) |
| 11 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001363059.1 | 33.1% | 31.5% | (many diffs) |
| 12 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001363061.1 | 33.1% | 31.5% | (many diffs) |
| 13 | human | 57509 | MTUS1 | microtubule associated scaf... | XM_011544595.3 | 32.8% | 31.3% | (many diffs) |
| 14 | human | 57509 | MTUS1 | microtubule associated scaf... | NM_001363057.2 | 32.7% | 26.6% | (many diffs) |
| 15 | human | 57509 | MTUS1 | microtubule associated scaf... | XM_005273578.3 | 32.7% | 26.6% | (many diffs) |
| 16 | human | 57509 | MTUS1 | microtubule associated scaf... | XM_017013698.2 | 32.7% | 26.6% | (many diffs) |
| 17 | human | 57509 | MTUS1 | microtubule associated scaf... | XM_017013699.1 | 32.7% | 26.6% | (many diffs) |
| 18 | human | 57509 | MTUS1 | microtubule associated scaf... | XM_024447213.1 | 32.7% | 26.6% | (many diffs) |
| 19 | human | 57509 | MTUS1 | microtubule associated scaf... | XR_001745567.2 | 19.5% | (many diffs) | |
| 20 | human | 57509 | MTUS1 | microtubule associated scaf... | NR_156437.1 | 19% | (many diffs) | |
| 21 | mouse | 102103 | Mtus1 | mitochondrial tumor suppres... | NM_001005865.3 | 83.5% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1377
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgttgtct cccaaattct ccttatccac cattcacata cgactgacgg 121 ccaaaggatt gcttcgaaac cttcgacttc cttcagggtt taggagaagc actgttgttt 181 tccacacagt tgaaaagagc aggcaaaaga atcctcgaag cttatgtatc cagccacaga 241 cagctcccga tgcgctgccc cctgagaaaa cacttgaatt gacgcaatat aaaacaaaat 301 gtgaaaacca aagtggattt atcctgcagc tcaagcagct tcttgcctgt ggtaatacca 361 agtttgaggc attgacagtt gtgattcagc acctgctgtc tgagcgggag gaagcactga 421 aacaacacaa aaccctatct caagaacttg ttaacctccg gggagagcta gtcactgctt 481 caaccacctg tgagaaatta gaaaaagcca ggaatgagtt acaaacagtg tatgaagcat 541 tcgtccagca gcaccaggct gaaaaaacag aacgagagaa tcggcttaaa gagttttaca 601 ccagggagta tgaaaagctt cgggacactt acattgaaga agcagagaag tacaaaatgc 661 aattgcaaga gcagtttgac aacttaaatg ctgcgcatga aacctctaag ttggaaattg 721 aagctagcca ctcagagaaa cttgaattgc taaagaaggc ctatgaagcc tccctttcag 781 aaattaagaa aggccatgaa atagaaaaga aatcgcttga agatttactt tctgagaagc 841 aggaatcgct agagaagcaa atcaatgatc tgaagagtga aaatgatgct ttaaatgaaa 901 aattgaaatc agaagaacaa aaaagaagag caagagaaaa agcaaatttg aaaaatcctc 961 agatcatgta tctagaacag gagttagaaa gcctgaaagc tgtgttagag atcaagaatg 1021 agaaactgca TCAACAGGAC ATCAAGTTAA TGAAAATGGA GAAACTGGTG GACAACAACA 1081 CAGCATTGGT TGACAAATTG AAGCGTTTCC AGCAGGAGAA TGAAGAATTG AAAGCTCGGA 1141 TGGACAAGCA CATGGCAATC TCAAGGCAGC TTTCCACGGA GCAGGCTGTT CTGCAAGAGT 1201 CGCTGGAGAA GGAGTCGAAA GTCAACAAGC GACTCTCTAT GGAAAACGAG GAGCTTCTGT 1261 GGAAACTGCA CAATGGGGAC CTGTGTAGCC CCAAGAGATC CCCCACATCC TCTGCCATCC 1321 CTTTGCAGTC ACCAAGGAAT TCGGGCTCCT TCCCTAGCCC CAGCATTTCA CCCAGATTGC 1381 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1441 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1501 ATATATCTTG TGGAAAGGAC GAAAGCCACT AAGTTGCCGG TGGCTAACGC GTTAAGTCga 1561 caatcaacct ctggattaca aaatttgtga aagatt